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- PDB-3wn0: Crystal Structure of Streptomyces coelicolor alpha-L-arabinofuran... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3wn0 | |||||||||
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Title | Crystal Structure of Streptomyces coelicolor alpha-L-arabinofuranosidase in complex with L-arabinose | |||||||||
![]() | Extracellular exo-alpha-L-arabinofuranosidase | |||||||||
![]() | HYDROLASE / five-bladed beta-propeller / Glycoside Hydrolase | |||||||||
Function / homology | ![]() L-arabinose metabolic process / non-reducing end alpha-L-arabinofuranosidase / alpha-L-arabinofuranosidase activity / xylan catabolic process / carbohydrate binding / extracellular region Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Fujimoto, Z. / Maehara, T. / Ichinose, H. / Michikawa, M. / Harazono, K. / Kaneko, S. | |||||||||
![]() | ![]() Title: Crystal structure and characterization of the glycoside hydrolase family 62 alpha-L-arabinofuranosidase from Streptomyces coelicolor Authors: Maehara, T. / Fujimoto, Z. / Ichinose, H. / Michikawa, M. / Harazono, K. / Kaneko, S. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 84.2 KB | Display | ![]() |
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PDB format | ![]() | 60.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 458.7 KB | Display | ![]() |
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Full document | ![]() | 459 KB | Display | |
Data in XML | ![]() | 17.1 KB | Display | |
Data in CIF | ![]() | 26.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3wmySC ![]() 3wmzC ![]() 3wn1C ![]() 3wn2C S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
-Protein / Sugars , 2 types, 2 molecules A![](data/chem/img/FUB.gif)
![](data/chem/img/FUB.gif)
#1: Protein | Mass: 46524.070 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() References: UniProt: O54161, non-reducing end alpha-L-arabinofuranosidase |
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#4: Sugar | ChemComp-FUB / |
-Non-polymers , 4 types, 378 molecules ![](data/chem/img/CA.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/CIT.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/CIT.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-CA / |
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#3: Chemical | ChemComp-CL / |
#5: Chemical | ChemComp-CIT / |
#6: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.68 Å3/Da / Density % sol: 54.16 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8.3 Details: 20% PEG 3350, 0.2M tri-potassium citrate, pH 8.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 95 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Feb 14, 2011 |
Radiation | Monochromator: Si(111) double crystal monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→29.75 Å / Num. obs: 40681 / % possible obs: 99.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 12 % / Rsym value: 0.106 / Net I/σ(I): 20.4 |
Reflection shell | Resolution: 1.9→1.97 Å / Redundancy: 11.3 % / Mean I/σ(I) obs: 7.2 / Rsym value: 0.433 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3WMY Resolution: 1.9→29.75 Å / Cor.coef. Fo:Fc: 0.955 / Cor.coef. Fo:Fc free: 0.95 / SU B: 1.97 / SU ML: 0.058 / Cross valid method: THROUGHOUT / ESU R: 0.107 / ESU R Free: 0.096 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 17.001 Å2
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Refine analyze | Luzzati coordinate error obs: 0.058 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→29.75 Å
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Refine LS restraints |
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