Entry Database : PDB / ID : 3wih Structure visualization Downloads & linksTitle Crystal structure of the third fibronectin domain (Fn3) of human ROBO1 in complex with the Fab fragment of murine monoclonal antibody B2212A. ComponentsRoundabout homolog 1 anti-human ROBO1 antibody B2212A Fab heavy chain anti-human ROBO1 antibody B2212A Fab light chain DetailsKeywords IMMUNE SYSTEM / fibronectin type-III domain / hepatocellular carcinoma antigen / angiogenesisFunction / homology Function and homology informationFunction Domain/homology Component
chemorepulsion involved in postnatal olfactory bulb interneuron migration / negative regulation of negative chemotaxis / Regulation of cortical dendrite branching / negative regulation of mammary gland epithelial cell proliferation / LRR domain binding / negative regulation of chemokine-mediated signaling pathway / heart induction / axon guidance receptor activity / positive regulation of vascular endothelial growth factor signaling pathway / Netrin-1 signaling ... chemorepulsion involved in postnatal olfactory bulb interneuron migration / negative regulation of negative chemotaxis / Regulation of cortical dendrite branching / negative regulation of mammary gland epithelial cell proliferation / LRR domain binding / negative regulation of chemokine-mediated signaling pathway / heart induction / axon guidance receptor activity / positive regulation of vascular endothelial growth factor signaling pathway / Netrin-1 signaling / Role of ABL in ROBO-SLIT signaling / Regulation of commissural axon pathfinding by SLIT and ROBO / SLIT2:ROBO1 increases RHOA activity / Roundabout signaling pathway / Inactivation of CDC42 and RAC1 / endocardial cushion formation / Signaling by ROBO receptors / pulmonary valve morphogenesis / outflow tract septum morphogenesis / aortic valve morphogenesis / positive regulation of vascular endothelial growth factor receptor signaling pathway / cell migration involved in sprouting angiogenesis / axon midline choice point recognition / Activation of RAC1 / aorta development / positive regulation of axonogenesis / positive regulation of Rho protein signal transduction / ventricular septum morphogenesis / positive regulation of Notch signaling pathway / homophilic cell adhesion via plasma membrane adhesion molecules / endoplasmic reticulum-Golgi intermediate compartment membrane / negative regulation of cell migration / synapse organization / positive regulation of MAP kinase activity / Regulation of expression of SLITs and ROBOs / activation of cysteine-type endopeptidase activity involved in apoptotic process / nervous system development / cell adhesion / neuron projection / axon / negative regulation of gene expression / neuronal cell body / positive regulation of gene expression / cell surface / identical protein binding / plasma membrane / cytoplasm Similarity search - Function Roundabout homologue 1 / Immunoglobulin domain / Immunoglobulin I-set / Immunoglobulin I-set domain / Fibronectin type III domain / Fibronectin type 3 domain / Immunoglobulin subtype 2 / Immunoglobulin C-2 Type / Immunoglobulin V-Type / Fibronectin type-III domain profile. ... Roundabout homologue 1 / Immunoglobulin domain / Immunoglobulin I-set / Immunoglobulin I-set domain / Fibronectin type III domain / Fibronectin type 3 domain / Immunoglobulin subtype 2 / Immunoglobulin C-2 Type / Immunoglobulin V-Type / Fibronectin type-III domain profile. / Fibronectin type III / Fibronectin type III superfamily / Immunoglobulin V-set domain / Immunoglobulin subtype / Immunoglobulin / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulins / Immunoglobulin-like fold / Immunoglobulin-like / Sandwich / Mainly Beta Similarity search - Domain/homologyBiological species Homo sapiens (human)Mus musculus (house mouse)Method X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution : 1.701 Å DetailsAuthors Nakayama, T. / Mizohata, E. / Yamashita, T. / Nagatoishi, M. / Iwanari, H. / Mochizuki, Y. / Kado, Y. / Yokota, Y. / Sato, R. / Tsumoto, K. ...Nakayama, T. / Mizohata, E. / Yamashita, T. / Nagatoishi, M. / Iwanari, H. / Mochizuki, Y. / Kado, Y. / Yokota, Y. / Sato, R. / Tsumoto, K. / Fujitani, H. / Kodama, T. / Hamakubo, T. / Inoue, T. CitationJournal : Protein Sci. / Year : 2015Title : Structural features of interfacial tyrosine residue in ROBO1 fibronectin domain-antibody complex: Crystallographic, thermodynamic, and molecular dynamic analysesAuthors : Nakayama, T. / Mizohata, E. / Yamashita, T. / Nagatoishi, S. / Nakakido, M. / Iwanari, H. / Mochizuki, Y. / Kado, Y. / Yokota, Y. / Satoh, R. / Tsumoto, K. / Fujitani, H. / Kodama, T. / Hamakubo, T. / Inoue, T. History Deposition Sep 12, 2013 Deposition site : PDBJ / Processing site : PDBJRevision 1.0 Jan 21, 2015 Provider : repository / Type : Initial releaseRevision 1.1 Mar 4, 2015 Group : Database referencesRevision 1.2 Nov 22, 2017 Group : Refinement description / Category : software / Item : _software.classification / _software.nameRevision 1.3 Nov 8, 2023 Group : Data collection / Database references ... Data collection / Database references / Derived calculations / Refinement description Category : chem_comp_atom / chem_comp_bond ... chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_site Item : _database_2.pdbx_DOI / _database_2.pdbx_database_accession ... _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id
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