+Open data
-Basic information
Entry | Database: PDB / ID: 3wi3 | ||||||
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Title | Crystal Structure of the Sld3/Treslin domain from yeast Sld3 | ||||||
Components | DNA replication regulator SLD3 | ||||||
Keywords | REPLICATION REGULATOR / CDC45-BINDING / ALPHA HELICAL | ||||||
Function / homology | Function and homology information chromatin => GO:0000785 / DNA replication preinitiation complex / double-strand break repair via break-induced replication / DNA unwinding involved in DNA replication / chromosome, centromeric region / DNA replication initiation / cell cycle / chromatin binding / nucleus Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.4 Å | ||||||
Authors | Itou, H. / Araki, H. / Shirakihara, Y. | ||||||
Citation | Journal: Structure / Year: 2014 Title: Crystal structure of the homology domain of the eukaryotic DNA replication proteins sld3/treslin. Authors: Itou, H. / Muramatsu, S. / Shirakihara, Y. / Araki, H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3wi3.cif.gz | 279.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3wi3.ent.gz | 228.2 KB | Display | PDB format |
PDBx/mmJSON format | 3wi3.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 3wi3_validation.pdf.gz | 495.6 KB | Display | wwPDB validaton report |
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Full document | 3wi3_full_validation.pdf.gz | 524.2 KB | Display | |
Data in XML | 3wi3_validation.xml.gz | 29.8 KB | Display | |
Data in CIF | 3wi3_validation.cif.gz | 40.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wi/3wi3 ftp://data.pdbj.org/pub/pdb/validation_reports/wi/3wi3 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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2 |
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3 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: _ / Refine code: _
NCS ensembles :
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-Components
#1: Protein | Mass: 34243.883 Da / Num. of mol.: 3 / Fragment: UNP RESIDUES 148-430 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Strain: ATCC 204508 / S288c / Gene: SLD3, YGL113W, G2980 / Plasmid: pET26 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P53135 #2: Chemical | #3: Chemical | ChemComp-EDO / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.37 Å3/Da / Density % sol: 48.13 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.4 Details: PEG 4000, LiSO4, pH 8.4, vapor diffusion, hanging drop, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Photon Factory / Beamline: AR-NE3A / Wavelength: 1 Å |
Detector | Type: ADSC / Detector: CCD / Date: Oct 31, 2012 |
Radiation | Monochromator: Si 111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→50 Å / Num. all: 39283 / Num. obs: 34298 / % possible obs: 99.2 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 10.8 % / Rmerge(I) obs: 0.065 / Net I/σ(I): 34.6 |
Reflection shell | Resolution: 2.4→2.44 Å / Redundancy: 6.5 % / Rmerge(I) obs: 0.4 / Mean I/σ(I) obs: 3.7 / % possible all: 98 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 2.4→19.64 Å / Cor.coef. Fo:Fc: 0.925 / Cor.coef. Fo:Fc free: 0.896 / Occupancy max: 1 / Occupancy min: 0.01 / SU B: 13.162 / SU ML: 0.155 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.396 / ESU R Free: 0.274 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES: WITH TLS ADDED
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 181.16 Å2 / Biso mean: 49.3721 Å2 / Biso min: 2 Å2
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Refinement step | Cycle: LAST / Resolution: 2.4→19.64 Å
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Refine LS restraints |
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Refine LS restraints NCS | Refine-ID: X-RAY DIFFRACTION / Type: INTERATOMIC DISTANCE / Weight position: 0.05
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LS refinement shell | Resolution: 2.4→2.461 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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