|Entry||Database: PDB / ID: 3v1g|
|Title||Forestalling insulin fibrillation by insertion of a chiral clamp mechanism-based application of protein engineering to global health|
|Components||(Insulin) x 2|
|Keywords||HORMONE / zinc-binding site / long-acting insulin analog / receptor binding protein engineering / insulin fibrillation|
|Function / homology|
Function and homology information
negative regulation of glycogen catabolic process / Signaling by Insulin receptor / regulation of cellular amino acid metabolic process / IRS activation / negative regulation of NAD(P)H oxidase activity / Insulin processing / nitric oxide-cGMP-mediated signaling pathway / Insulin receptor recycling / negative regulation of fatty acid metabolic process / negative regulation of feeding behavior ...negative regulation of glycogen catabolic process / Signaling by Insulin receptor / regulation of cellular amino acid metabolic process / IRS activation / negative regulation of NAD(P)H oxidase activity / Insulin processing / nitric oxide-cGMP-mediated signaling pathway / Insulin receptor recycling / negative regulation of fatty acid metabolic process / negative regulation of feeding behavior / regulation of protein secretion / Regulation of gene expression in beta cells / positive regulation of peptide hormone secretion / positive regulation of respiratory burst / negative regulation of respiratory burst involved in inflammatory response / alpha-beta T cell activation / negative regulation of gluconeogenesis / negative regulation of acute inflammatory response / Synthesis, secretion, and deacylation of Ghrelin / positive regulation of dendritic spine maintenance / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / positive regulation of glycogen biosynthetic process / negative regulation of reactive oxygen species biosynthetic process / regulation of protein localization to plasma membrane / COPI-mediated anterograde transport / negative regulation of protein secretion / Signal attenuation / positive regulation of nitric oxide mediated signal transduction / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / positive regulation of lipid biosynthetic process / negative regulation of lipid catabolic process / fatty acid homeostasis / endosome lumen / positive regulation of insulin receptor signaling pathway / Regulation of insulin secretion / endoplasmic reticulum-Golgi intermediate compartment membrane / transport vesicle / positive regulation of protein autophosphorylation / insulin-like growth factor receptor binding / neuron projection maintenance / positive regulation of protein metabolic process / positive regulation of glycolytic process / Insulin receptor signalling cascade / positive regulation of brown fat cell differentiation / positive regulation of mitotic nuclear division / positive regulation of long-term synaptic potentiation / regulation of transmembrane transporter activity / positive regulation of cell differentiation / regulation of synaptic plasticity / activation of protein kinase B activity / cognition / positive regulation of cytokine production / positive regulation of protein secretion / acute-phase response / positive regulation of glucose import / negative regulation of proteolysis / positive regulation of nitric-oxide synthase activity / hormone activity / insulin receptor binding / vasodilation / negative regulation of protein catabolic process / insulin receptor signaling pathway / positive regulation of protein localization to nucleus / wound healing / Golgi lumen / regulation of protein localization / glucose metabolic process / glucose homeostasis / cell-cell signaling / positive regulation of phosphatidylinositol 3-kinase signaling / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / positive regulation of cell growth / secretory granule lumen / positive regulation of NF-kappaB transcription factor activity / positive regulation of protein kinase B signaling / positive regulation of MAPK cascade / protease binding / G protein-coupled receptor signaling pathway / positive regulation of cell migration / Amyloid fiber formation / Golgi membrane / negative regulation of gene expression / endoplasmic reticulum lumen / positive regulation of cell population proliferation / positive regulation of gene expression / regulation of DNA-templated transcription / extracellular space / extracellular region / identical protein binding
Similarity search - Function
Insulin / Insulin family / Insulin / insulin-like growth factor / relaxin family. / Insulin/IGF/Relaxin family / Insulin-like / Insulin family signature. / Insulin-like superfamily / Insulin, conserved site
Similarity search - Domain/homology
PHENOL / Insulin
Similarity search - Component
|Biological species||Homo sapiens (human)|
|Method||X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.2 Å|
|Authors||Wan, Z.L. / Hua, Q.X. / Wickramasinghe, N.P. / Huang, K. / Petkova, A.T. / Hu, S.Q. / Phillips, N.B. / Yeh, I.J. / Whittake, J. / Ismail-Beigi, F. ...Wan, Z.L. / Hua, Q.X. / Wickramasinghe, N.P. / Huang, K. / Petkova, A.T. / Hu, S.Q. / Phillips, N.B. / Yeh, I.J. / Whittake, J. / Ismail-Beigi, F. / Katsoyyannis, P.G. / Tycko, R. / Weiss, M.A.|
Journal: To be Published
Title: Forestalling insulin fibrillation by insertion of a chiral clamp mechanism-based application of protein engineering to global health
Authors: Wan, Z.L. / Hua, Q.X. / Wickramasinghe, N.P. / Huang, K. / Petkova, A.T. / Hu, S.Q. / Phillips, N.B. / Yeh, I.J. / Whittake, J. / Ismail-Beigi, F. / Katsoyyannis, P.G. / Tycko, R. / Weiss, M.A.
#1: Journal: Biochemistry / Year: 2005
Title: Diabetes-associated mutations in human insulin: crystal structure and photo-cross-linking studies of a-chain variant insulin Wakayama
Authors: Wan, Z.L. / Huang, K. / Xu, B. / Hu, S.Q. / Wang, S. / Chu, Y.C. / Katsoyannis, P.G. / Weiss, M.A.
#2: Journal: Biochemistry / Year: 2003
Title: Crystal structure of allo-Ile(A2)-insulin, an inactive chiral analogue: implications for the mechanism of receptor binding
Authors: Wan, Z.L. / Xu, B. / Chu, Y.C. / Katsoyannis, P.G. / Weiss, M.A.
#3: Journal: Biochemistry / Year: 2004
Title: Enhancing the activity of insulin at the receptor interface: crystal structure and photo-cross-linking of A8 analogues
Authors: L Wan, Z. / Xu, B. / Chu, Y.C. / Li, B. / Nakagawa, S.H. / Qu, Y. / Hu, S.Q. / Katsoyannis, P.G. / Weiss, M.A.
#4: Journal: Philos.Trans.R.Soc.London,Ser.B / Year: 1988
Title: The structure of 2zn pig insulin crystal at 1.5 A resolution
Authors: Baker, E.N. / Blundell, T.L. / Cutfield, J.F. / Cutfield, S.M. / Dodson, E.J. / Dodson, G.G. / Hodgkin, D. / Isaacs, N.W. / Reynolds, C.D.
#5: Journal: Nature / Year: 1976
Title: Structure of insulin in 4-zinc insulin
Authors: Bentley, G. / Dodson, E. / Dodson, G. / Hodgkin, D. / Mercola, D.
|Structure viewer||Molecule: |
Downloads & links
|Components on special symmetry positions|
-Protein/peptide , 2 types, 4 molecules A
C B D
Mass: 2383.698 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) / References: UniProt: P01308
Mass: 3433.953 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) / References: UniProt: P01308
-Non-polymers , 4 types, 85 molecules
|#3: Chemical||#4: Chemical|| ChemComp-IPH / ||#5: Chemical|| ChemComp-CL / ||#6: Water|| ChemComp-HOH / |
|Experiment||Method: X-RAY DIFFRACTION / Number of used crystals: 1|
|Crystal||Density Matthews: 1.93 Å3/Da / Density % sol: 36.41 %|
|Crystal grow||Temperature: 298 K / Method: vapor diffusion / pH: 8 |
Details: 0.02 M Tris, 0.05 M sodium citrate, 5% acetone, 0.03% phenol, 0.01% zinc acetate, pH 8.0, VAPOR DIFFUSION, temperature 298K
|Diffraction||Mean temperature: 100 K|
|Diffraction source||Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.5418 Å|
|Detector||Type: Bruker AXIOM 200 / Detector: CCD / Date: Jan 14, 2005 / Details: Optics Mirrors|
|Radiation||Monochromator: Monochromator Mirror / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray|
|Radiation wavelength||Wavelength: 1.5418 Å / Relative weight: 1|
|Reflection||Resolution: 2.2→39.54 Å / Num. all: 4417 / Num. obs: 4321 / % possible obs: 98.4 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.2 % / Biso Wilson estimate: 32.2 Å2 / Rmerge(I) obs: 0.036 / Net I/σ(I): 16.9|
|Reflection shell||Resolution: 2.2→2.34 Å / Redundancy: 3 % / Rmerge(I) obs: 0.232 / Mean I/σ(I) obs: 3.7 / Num. unique all: 632 / % possible all: 100|
|Refinement||Method to determine structure: MOLECULAR REPLACEMENT|
Starting model: 1TRZ
Resolution: 2.2→39.54 Å / Isotropic thermal model: restrained / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
|Displacement parameters||Biso mean: 39.4 Å2|
|Refine analyze||Luzzati coordinate error obs: 0.28 Å / Luzzati d res low obs: 5 Å / Luzzati sigma a obs: 0.15 Å|
|Refinement step||Cycle: LAST / Resolution: 2.2→39.54 Å|
|Refine LS restraints|
|LS refinement shell||Resolution: 2.2→2.34 Å / Rfactor Rfree error: 0.052 |
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