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- PDB-3v0n: Crystal structure of the Fucosylgalactoside alpha N-acetylgalacto... -

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Basic information

Entry
Database: PDB / ID: 3v0n
TitleCrystal structure of the Fucosylgalactoside alpha N-acetylgalactosaminyltransferase (GTA, cisAB mutant L266G, G268A) in complex with a novel UDP-GalNAc derived inhibitor (3GW and 4GW)
ComponentsHisto-blood group ABO system transferase
KeywordsTRANSFERASE/TRANSFERASE INHIBITOR / GTA / ABO / CISAB MUTANT / ROSSMANN FOLD / "closed" CONFORMATION / GLYCOSYLTRANSFERASE / GLYCOPROTEIN / BLOOD GROUP ANTIGEN / UDP-GalNAc / METAL-BINDING / MANGANESE / Glycosylation / TRANSMEMBRANE / GOLGI APPARATUS / SECRETED / SIGNAL-ANCHOR / TRANSFERASE-TRANSFERASE INHIBITOR complex
Function / homology
Function and homology information


fucosylgalactoside 3-alpha-galactosyltransferase / glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase / glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase activity / fucosylgalactoside 3-alpha-galactosyltransferase activity / ABO blood group biosynthesis / lipid glycosylation / Golgi cisterna membrane / protein glycosylation / antigen binding / manganese ion binding ...fucosylgalactoside 3-alpha-galactosyltransferase / glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase / glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase activity / fucosylgalactoside 3-alpha-galactosyltransferase activity / ABO blood group biosynthesis / lipid glycosylation / Golgi cisterna membrane / protein glycosylation / antigen binding / manganese ion binding / vesicle / carbohydrate metabolic process / Golgi membrane / nucleotide binding / Golgi apparatus / extracellular region
Similarity search - Function
Glycosyl transferase, family 6 / Glycosyltransferase family 6 / Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A / Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A / Nucleotide-diphospho-sugar transferases / Alpha-Beta Complex / Alpha Beta
Similarity search - Domain/homology
Chem-3GW / Chem-4GW / : / Histo-blood group ABO system transferase
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 1.75 Å
AuthorsPalcic, M.M. / Jorgensen, R.
CitationJournal: J.Biol.Chem. / Year: 2013
Title: Base-modified donor analogues reveal novel dynamic features of a glycosyltransferase.
Authors: Jrgensen, R. / Pesnot, T. / Lee, H.J. / Palcic, M.M. / Wagner, G.K.
History
DepositionDec 8, 2011Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 23, 2013Provider: repository / Type: Initial release
Revision 1.1Nov 12, 2014Group: Structure summary
Revision 1.2Feb 18, 2015Group: Database references
Revision 1.3Nov 8, 2017Group: Data collection / Refinement description / Category: diffrn_source / software / Item: _diffrn_source.pdbx_synchrotron_site / _software.name
Revision 1.4Sep 13, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn / struct_ref_seq_dif / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_alt_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_alt_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_ptnr2_label_alt_id / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Histo-blood group ABO system transferase
B: Histo-blood group ABO system transferase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)71,63612
Polymers69,3082
Non-polymers2,32810
Water9,836546
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6700 Å2
ΔGint-72 kcal/mol
Surface area22360 Å2
MethodPISA
Unit cell
Length a, b, c (Å)78.200, 153.830, 52.400
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number18
Space group name H-MP21212
Components on special symmetry positions
IDModelComponents
11B-730-

HOH

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Components

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Protein , 1 types, 2 molecules AB

#1: Protein Histo-blood group ABO system transferase / Fucosylglycoprotein 3-alpha-galactosyltransferase / Fucosylglycoprotein alpha-N- ...Fucosylglycoprotein 3-alpha-galactosyltransferase / Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase / Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase / Glycoprotein-fucosylgalactoside alpha-galactosyltransferase / Histo-blood group A transferase / A transferase / Histo-blood group B transferase / B transferase / NAGAT / Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form


Mass: 34654.008 Da / Num. of mol.: 2 / Fragment: Extracellular catalytic domain / Mutation: L266G, G268A
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: AB0, ABO / Plasmid: PCW DELTA 1AC / Production host: Escherichia coli (E. coli) / Strain (production host): BL21
References: UniProt: P16442, glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, fucosylgalactoside 3-alpha-galactosyltransferase

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Non-polymers , 6 types, 556 molecules

#2: Chemical ChemComp-MN / MANGANESE (II) ION


Mass: 54.938 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mn
#3: Chemical ChemComp-4GW / 5-(5-formylthiophen-2-yl)uridine 5'-(trihydrogen diphosphate)


Mass: 514.295 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C14H16N2O13P2S
#4: Chemical ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C3H8O3
#5: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: SO4
#6: Chemical ChemComp-3GW / 5-(5-formylthiophen-2-yl)uridine-5'(2-(acetylamino)-2-deoxy-alpha-D-galactosyl)-diphosphate


Mass: 717.487 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C22H29N3O18P2S
#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 546 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.27 Å3/Da / Density % sol: 45.9 %
Crystal growTemperature: 295 K / Method: vapor diffusion, hanging drop / pH: 7
Details: 50 mM MOPS pH 7, 50-200 mM AMMONIUM SULFATE, 50 mM MnCl2, 6-9% PEG-3350 , VAPOR DIFFUSION, HANGING DROP, temperature 295K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: MAX II / Beamline: I911-2 / Wavelength: 1.03796 Å
DetectorType: MAR CCD 165 mm / Detector: CCD / Date: Mar 26, 2010
Details: Multilayer mirror, curved to focus in the vertical (R = 400 m)
RadiationMonochromator: Bent Si (111) crystal, horizontally focusing / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.03796 Å / Relative weight: 1
ReflectionResolution: 1.75→30 Å / Num. all: 64706 / Num. obs: 64658 / % possible obs: 99.9 % / Observed criterion σ(I): -3 / Redundancy: 5.6 % / Biso Wilson estimate: 26.278 Å2 / Rmerge(I) obs: 0.069 / Net I/σ(I): 17.37
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. measured obsNum. unique allNum. unique obs% possible all
1.75-1.860.6193.062832246884688100
1.8-1.846.10.4883.842790546054604100
1.84-1.96.10.3864.81272614494449099.9
1.9-1.966.10.2856.432642743514351100
1.96-2.026.10.2228.072563142044203100
2.02-2.096.10.1799.82503741074107100
2.09-2.176.10.14911.462411339513950100
2.17-2.266.10.1213.862319537963796100
2.26-2.366.10.10415.632237436603659100
2.36-2.476.10.09916.252149235093509100
2.47-2.616.10.07719.842047033473346100
2.61-2.776.10.06822.041933531593159100
2.77-2.966.10.05825.361816929742973100
2.96-3.26.10.04531.191697227802780100
3.2-3.56.10.03736.881574625882588100
3.5-3.9160.03740.871413523482348100
3.91-4.5260.03144.321244020862085100
4.52-5.535.90.03243.94105401788178799.9
5.53-7.835.70.03940.9880921424142099.7
7.83-305.10.02745.08432684781596.2

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Phasing

PhasingMethod: molecular replacement
Phasing MRRfactor: 31.56 / Model details: Phaser MODE: MR_AUTO
Highest resolutionLowest resolution
Rotation2.5 Å29.02 Å
Translation2.5 Å29.02 Å

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Processing

Software
NameVersionClassificationNB
XSCALEdata scaling
PHASER2.1.4phasing
PHENIX1.7.1_743refinement
PDB_EXTRACT3.1data extraction
MAR345data collection
XDSdata reduction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 2RIT

2rit


Resolution: 1.75→29.021 Å / Occupancy max: 1 / Occupancy min: 0.33 / FOM work R set: 0.9029 / SU ML: 0.34 / Isotropic thermal model: Isotropic+tls / Cross valid method: THROUGHOUT / σ(F): 2 / Phase error: 16.42 / Stereochemistry target values: MLHL
RfactorNum. reflection% reflectionSelection details
Rfree0.1843 2586 4 %Random
Rwork0.1571 ---
obs0.1582 64592 99.96 %-
Solvent computationShrinkage radii: 0.72 Å / VDW probe radii: 1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 45.549 Å2 / ksol: 0.384 e/Å3
Displacement parametersBiso max: 81.34 Å2 / Biso mean: 22.1828 Å2 / Biso min: 7.01 Å2
Baniso -1Baniso -2Baniso -3
1-5.207 Å20 Å20 Å2
2---1.2905 Å20 Å2
3----3.9166 Å2
Refinement stepCycle: LAST / Resolution: 1.75→29.021 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4720 0 139 546 5405
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.015054
X-RAY DIFFRACTIONf_angle_d1.2466890
X-RAY DIFFRACTIONf_chiral_restr0.089738
X-RAY DIFFRACTIONf_plane_restr0.007862
X-RAY DIFFRACTIONf_dihedral_angle_d16.4151960
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 18 / % reflection obs: 100 %

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection allNum. reflection obs
1.75-1.78370.25971410.2315337235133513
1.7837-1.82010.24661420.2121341235543554
1.8201-1.85960.23581410.1942338735283528
1.8596-1.90290.19261410.1794339035313531
1.9029-1.95050.17521430.162340935523552
1.9505-2.00320.1971420.1521341635583558
2.0032-2.06210.17911420.1507339735393539
2.0621-2.12870.18651420.1518341035523552
2.1287-2.20470.17991430.1498342335663566
2.2047-2.2930.1831430.1436345035933593
2.293-2.39730.181430.1508341135543554
2.3973-2.52360.20181430.1552343235753575
2.5236-2.68160.20551430.1513344435873587
2.6816-2.88850.20591450.163347136163616
2.8885-3.17880.18461440.1557345435983598
3.1788-3.63810.1521460.1479350336493649
3.6381-4.58070.15611480.1316353236803680
4.5807-29.0250.18531540.1755369338473847
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.68080.6128-0.41211.70890.89792.0195-0.1346-0.15560.0352-0.05850.2876-0.35680.22770.2453-0.12470.2397-0.0039-0.05440.20340.02570.162516.6401-14.2529-16.3085
20.86421.01711.09771.20051.30791.45470.0484-0.0428-0.1762-0.1680.14180.44060.00860.2602-0.11450.2277-0.0462-0.02390.1584-0.07050.2277-3.1839-15.8177-11.2438
30.9229-0.10180.50171.455-0.31721.8191-0.0457-0.1282-0.0730.24730.02410.2856-0.1782-0.17440.04140.09610.01430.01710.147-0.01580.13162.8848-26.4174-9.1787
43.6324-0.5637-0.37031.68690.36851.94370.04940.09520.1647-0.1105-0.0365-0.1283-0.24430.17570.01710.1143-0.0019-0.00350.11940.00420.100214.502-20.199-20.9907
51.79281.72190.39572.79820.44431.49950.03070.0296-0.01730.0598-0.0416-0.19090.08560.02150.03690.0421-0.00150.01620.07320.01240.107518.481-36.3151-23.8268
61.19830.04650.40762.9286-0.14881.39170.05520.1072-0.0895-0.3385-0.08030.07470.2302-0.10580.02220.1391-0.011-0.00460.146-0.01350.11418.753-41.5111-31.6607
70.7587-0.4861-0.19553.3374-0.13051.26010.06120.0352-0.0515-0.2555-0.1334-0.22440.14270.04470.05760.10640.01340.01910.10440.01910.132318.4965-40.3286-28.0405
81.54190.44030.0430.19540.18540.4333-0.090.0649-0.1446-0.8266-0.0375-0.11260.38810.26470.03850.38210.00280.06590.1522-0.02360.18714.8351-47.288-38.0744
90.52550.23610.50590.7379-0.3050.975-0.0968-0.0405-0.387-0.131-0.0276-0.05520.51260.0823-0.01370.21570.0240.0860.1240.01860.22218.9825-45.7725-29.4449
101.8080.0044-0.0910.20740.55232.7142-0.028-0.115-0.45690.1048-0.0721-0.17970.6769-0.1166-0.0630.19510.04140.01950.13770.03440.197117.2572-50.7181-18.3607
110.3751-0.0409-0.51721.4431-0.26810.8005-0.002-0.0056-0.0941-0.1125-0.00250.13650.0952-0.09560.01470.0658-0.00140.00340.0938-0.00480.11259.7278-37.9298-24.076
120.4444-0.24330.09350.41770.18640.54270.018-0.1146-0.09750.1347-0.02320.23520.0603-0.1422-0.02990.0701-0.00580.02310.13060.00860.14654.3267-34.411-13.8071
130.56950.4018-0.15111.1917-0.58660.9506-0.0692-0.0499-0.01910.17520.0560.17170.0712-0.0691-0.17380.2465-0.01380.17910.23880.07680.2393-1.5876-48.3744-2.8136
140.2283-0.08380.11660.031-0.04160.0596-0.0204-0.1891-0.19880.16110.07680.16520.1891-0.08850.33980.3663-0.04730.13770.18020.06220.17982.8733-47.36131.2279
150.7847-0.2547-0.18030.57760.01340.83280.0139-0.0317-0.03760.1268-0.03450.0850.1684-0.05230.01090.0975-0.02670.03170.12540.01290.12038.3821-39.3224-13.0247
164.5248-1.3793-1.52793.4245-1.66132.02180.206-0.6245-0.02620.8372-0.0587-0.5970.1140.8799-0.08740.1846-0.0049-0.0570.20180.00310.168621.4344-40.5056-10.1297
171.60170.9368-1.26896.1243-2.83033.01230.02220.0113-0.3415-0.0168-0.1768-0.35390.30120.17620.0830.2173-0.0085-0.01020.16540.01910.182115.244-53.0917-7.6955
181.29450.41130.09640.62430.12820.7690.0911-0.1722-0.4990.13950.01650.04080.4382-0.14660.74960.1091-0.08570.1239-0.00020.1225-0.10937.7317-46.1266-6.8056
191.22480.3750.11020.1281-0.01170.5589-0.0102-0.12420.0887-0.00390.00620.26530.0102-0.15650.04410.0372-0.031-0.02030.1612-0.01220.2155-4.5359-35.1144-21.0314
201.24240.57660.5091.1514-0.1911.3053-0.07850.106-0.1915-0.0533-0.02030.07520.0113-0.21520.06480.0487-0.0099-0.03540.1374-0.04480.1955-1.091-33.6574-24.6152
210.9637-0.58170.80820.97290.5992.5775-0.0706-0.08130.00940.18250.2163-0.11510.05310.26210.04540.414-0.0480.10090.21060.00950.167710.0211-35.43871.0762
221.70733.67381.9497.92344.21092.2390.0607-0.02490.09230.5146-0.10870.27290.57970.0430.07690.2688-0.05670.0410.336-0.1010.2895-5.3665-22.6928-3.8326
230.4335-0.2049-0.41910.7002-0.18860.6652-0.0105-0.0132-0.0209-0.0103-0.08040.15080.061-0.10020.02530.0522-0.01340.01790.1624-0.01910.11165.6884-17.5644-6.0345
243.6362-0.89070.95370.96990.1051.67630.1906-0.2163-0.0797-0.0299-0.0881-0.02440.4932-0.0677-0.07540.1895-0.01580.01790.1449-0.00440.105711.7042-29.38795.7142
251.34161.2273-0.10876.09550.7551.1224-0.0766-0.0463-0.14310.22120.1033-0.23460.12310.10990.0080.1566-0.0287-0.03440.14010.03150.094224.2151-18.06888.3511
261.109-0.0343-0.02410.72350.39980.88340.1048-0.19210.13340.3166-0.1542-0.0024-0.13030.09680.05520.1948-0.0441-0.01560.1272-0.00390.09319.1571-8.712316.4964
271.57012.003-0.17583.2690.2750.36950.0841-0.237-0.12-0.0702-0.2827-0.2926-0.02570.1628-0.00430.1862-0.0603-0.05520.13360.04790.1426.511-15.346412.7373
280.7180.77790.34761.51440.55550.21540.1108-0.1810.03750.3762-0.2-0.00840.04080.0066-0.38390.3448-0.1052-0.09130.15380.03560.142827.4772-7.401822.8574
290.8089-0.0581-0.19161.10680.21250.71390.1837-0.07560.07990.4445-0.2645-0.3489-0.35090.23310.08190.2975-0.0708-0.06410.18150.04450.191129.9137-10.899814.1777
301.8980.42130.13.5999-0.12292.30320.0903-0.14790.05520.1955-0.0984-0.5049-0.17890.28530.01720.175-0.0557-0.02750.27260.02610.213931.3503-6.10343.2206
310.72940.51450.18641.2371-0.02071.06160.0587-0.05640.13150.139-0.09420.0159-0.04720.02020.04610.1309-0.0261-0.01630.15960.0070.123417.9188-12.14088.8568
321.22420.4509-0.18280.94680.09060.99530.03060.08530.1770.0814-0.06670.1123-0.1354-0.10790.01640.1188-0.0093-0.00210.1368-0.00980.119811.4936-11.8839-1.4186
331.5952-0.4472-1.1131.3385-0.1882.9253-0.00230.44710.395-0.36750.03770.3785-0.5363-0.16060.07190.2280.0082-0.06660.1520.04660.221214.7262.9839-12.53
341.48391.0020.30431.7518-0.4030.9341-0.3560.1710.2278-0.38430.1373-0.0359-0.29180.22060.16640.235-0.0534-0.04910.17270.0520.155817.7221-0.357-16.4505
351.3588-0.61410.50491.746-0.29771.0717-0.0244-0.05920.16450.3176-0.1131-0.0923-0.17390.07380.12140.1319-0.0259-0.01590.12040.01520.107417.5946-10.1839-2.2279
363.40521.68410.85116.1297-1.35762.91790.08890.193-0.395-0.58190.1254-0.72060.42631.0381-0.2060.15770.01970.00310.25230.01440.213428.9337-16.5489-5.2231
371.09421.3001-1.21853.2194-1.71111.3990.01190.08010.29250.1819-0.086-0.4693-0.45680.4935-0.14040.1809-0.0891-0.03170.21440.0320.203231.0606-2.6531-7.5378
381.0870.34960.07091.7406-0.25830.5449-0.02870.04930.20310.0347-0.06530.034-0.19180.09480.09010.1644-0.0272-0.03230.14880.02710.13920.7723-4.3995-8.3642
392.3412-1.5864-0.6662.0150.00680.6133-0.09410.14380.16890.30780.06610.05660.0334-0.05220.0360.14320.01480.0110.1333-0.00880.1614.7091-6.61875.7452
402.97850.4949-0.11022.46870.01192.3892-0.0352-0.02390.09580.2389-0.05020.1965-0.0249-0.31740.08360.1470.01110.03440.1419-0.03270.18036.6186-9.44529.4644
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1(chain A and resid 58:76)A58 - 76
2X-RAY DIFFRACTION2(chain A and resid 77:82)A77 - 82
3X-RAY DIFFRACTION3(chain A and resid 83:98)A83 - 98
4X-RAY DIFFRACTION4(chain A and resid 99:111)A99 - 111
5X-RAY DIFFRACTION5(chain A and resid 112:122)A112 - 122
6X-RAY DIFFRACTION6(chain A and resid 123:143)A123 - 143
7X-RAY DIFFRACTION7(chain A and resid 144:154)A144 - 154
8X-RAY DIFFRACTION8(chain A and resid 155:163)A155 - 163
9X-RAY DIFFRACTION9(chain A and resid 164:178)A164 - 178
10X-RAY DIFFRACTION10(chain A and resid 179:203)A179 - 203
11X-RAY DIFFRACTION11(chain A and resid 204:218)A204 - 218
12X-RAY DIFFRACTION12(chain A and resid 219:241)A219 - 241
13X-RAY DIFFRACTION13(chain A and resid 242:250)A242 - 250
14X-RAY DIFFRACTION14(chain A and resid 251:262)A251 - 262
15X-RAY DIFFRACTION15(chain A and resid 263:274)A263 - 274
16X-RAY DIFFRACTION16(chain A and resid 275:279)A275 - 279
17X-RAY DIFFRACTION17(chain A and resid 280:293)A280 - 293
18X-RAY DIFFRACTION18(chain A and resid 294:318)A294 - 318
19X-RAY DIFFRACTION19(chain A and resid 319:335)A319 - 335
20X-RAY DIFFRACTION20(chain A and resid 336:345)A336 - 345
21X-RAY DIFFRACTION21(chain B and resid 58:76)B58 - 76
22X-RAY DIFFRACTION22(chain B and resid 77:82)B77 - 82
23X-RAY DIFFRACTION23(chain B and resid 83:98)B83 - 98
24X-RAY DIFFRACTION24(chain B and resid 99:111)B99 - 111
25X-RAY DIFFRACTION25(chain B and resid 112:122)B112 - 122
26X-RAY DIFFRACTION26(chain B and resid 123:143)B123 - 143
27X-RAY DIFFRACTION27(chain B and resid 144:154)B144 - 154
28X-RAY DIFFRACTION28(chain B and resid 155:163)B155 - 163
29X-RAY DIFFRACTION29(chain B and resid 164:178)B164 - 178
30X-RAY DIFFRACTION30(chain B and resid 179:203)B179 - 203
31X-RAY DIFFRACTION31(chain B and resid 204:218)B204 - 218
32X-RAY DIFFRACTION32(chain B and resid 219:241)B219 - 241
33X-RAY DIFFRACTION33(chain B and resid 242:250)B242 - 250
34X-RAY DIFFRACTION34(chain B and resid 251:262)B251 - 262
35X-RAY DIFFRACTION35(chain B and resid 263:274)B263 - 274
36X-RAY DIFFRACTION36(chain B and resid 275:279)B275 - 279
37X-RAY DIFFRACTION37(chain B and resid 280:293)B280 - 293
38X-RAY DIFFRACTION38(chain B and resid 294:318)B294 - 318
39X-RAY DIFFRACTION39(chain B and resid 319:335)B319 - 335
40X-RAY DIFFRACTION40(chain B and resid 336:345)B336 - 345

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