- PDB-3ioi: Crystal structure of the Fucosylgalactoside alpha N-acetylgalacto... -
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Basic information
Entry
Database: PDB / ID: 3ioi
Title
Crystal structure of the Fucosylgalactoside alpha N-acetylgalactosaminyltransferase (GTA, cisAB mutant L266G, G268A) in complex with a novel UDP-Gal derived inhibitor (1GW)
Mass: 18.015 Da / Num. of mol.: 224 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.21 Å3/Da / Density % sol: 44.25 %
Crystal grow
Temperature: 292 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 50 mM MOPS pH 7, 50-200 mM ammonium sulfate, 50 mM MnCl2, and 6-9% PEG-3350, VAPOR DIFFUSION, HANGING DROP, temperature 292K
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Data collection
Diffraction
Mean temperature: 100 K
Diffraction source
Source: SYNCHROTRON / Site: MAX II / Beamline: I911-5 / Wavelength: 0.90772 Å
Detector
Type: MAR555 FLAT PANEL / Detector: IMAGE PLATE / Date: May 29, 2009 Details: Multilayer mirror, curved to focus in the vertical (R = 400 m)
Radiation
Monochromator: Bent Si (220) crystal, horizontally focusing / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 0.90772 Å / Relative weight: 1
Reflection
Resolution: 1.45→20 Å / Num. obs: 54487 / % possible obs: 99.6 % / Observed criterion σ(I): -3 / Redundancy: 11.07 % / Biso Wilson estimate: 22.001 Å2 / Rmerge(I) obs: 0.084 / Net I/σ(I): 17.34