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- PDB-3v0m: Crystal structure of the Fucosylgalactoside alpha N-acetylgalacto... -

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Basic information

Entry
Database: PDB / ID: 3v0m
TitleCrystal structure of the Fucosylgalactoside alpha N-acetylgalactosaminyltransferase (GTA, cisAB mutant L266G, G268A) in complex with a novel UDP-Gal derived inhibitor (5GW) and H-antigen acceptor
ComponentsHisto-blood group ABO system transferase
KeywordsTRANSFERASE/TRANSFERASE INHIBITOR / GTA / ABO / CISAB MUTANT / AA(GLY)B / ROSSMANN FOLD / "closed" CONFORMATION / GLYCOSYLTRANSFERASE / GLYCOPROTEIN / BLOOD GROUP ANTIGEN / UDP-GalNAc / METAL-BINDING / MANGANESE / Glycosylation / TRANSMEMBRANE / GOLGI APPARATUS / SECRETED / SIGNAL-ANCHOR / TRANSFERASE-TRANSFERASE INHIBITOR complex
Function / homology
Function and homology information


fucosylgalactoside 3-alpha-galactosyltransferase / glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase / glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase activity / fucosylgalactoside 3-alpha-galactosyltransferase activity / ABO blood group biosynthesis / : / Golgi cisterna membrane / : / antigen binding / manganese ion binding ...fucosylgalactoside 3-alpha-galactosyltransferase / glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase / glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase activity / fucosylgalactoside 3-alpha-galactosyltransferase activity / ABO blood group biosynthesis / : / Golgi cisterna membrane / : / antigen binding / manganese ion binding / vesicle / carbohydrate metabolic process / Golgi membrane / nucleotide binding / Golgi apparatus / extracellular region
Similarity search - Function
Glycosyl transferase, family 6 / Glycosyltransferase family 6 / Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A / Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A / Nucleotide-diphospho-sugar transferases / Alpha-Beta Complex / Alpha Beta
Similarity search - Domain/homology
5-phenyluridine 5'-(trihydrogen diphosphate) / Chem-BHE / : / Histo-blood group ABO system transferase
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 1.68 Å
AuthorsPalcic, M.M. / Jorgensen, R.
CitationJournal: J.Biol.Chem. / Year: 2013
Title: Base-modified Donor Analogues Reveal Novel Dynamic Features of a Glycosyltransferase.
Authors: Jrgensen, R. / Pesnot, T. / Lee, H.J. / Palcic, M.M. / Wagner, G.K.
History
DepositionDec 8, 2011Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 23, 2013Provider: repository / Type: Initial release
Revision 1.1Aug 28, 2013Group: Database references
Revision 1.2Sep 25, 2013Group: Database references
Revision 1.3Nov 12, 2014Group: Structure summary
Revision 1.4Nov 8, 2017Group: Data collection / Refinement description / Category: diffrn_source / software / Item: _diffrn_source.pdbx_synchrotron_site / _software.name
Revision 1.5Sep 13, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn / struct_ref_seq_dif / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Histo-blood group ABO system transferase
B: Histo-blood group ABO system transferase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)71,3519
Polymers69,3082
Non-polymers2,0437
Water9,764542
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area5890 Å2
ΔGint-52 kcal/mol
Surface area22180 Å2
MethodPISA
Unit cell
Length a, b, c (Å)78.170, 153.250, 52.720
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number18
Space group name H-MP21212
Components on special symmetry positions
IDModelComponents
11B-537-

HOH

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Components

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Protein , 1 types, 2 molecules AB

#1: Protein Histo-blood group ABO system transferase / Fucosylglycoprotein 3-alpha-galactosyltransferase / Fucosylglycoprotein alpha-N- ...Fucosylglycoprotein 3-alpha-galactosyltransferase / Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase / Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase / Glycoprotein-fucosylgalactoside alpha-galactosyltransferase / Histo-blood group A transferase / A transferase / Histo-blood group B transferase / B transferase / NAGAT / Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form


Mass: 34654.008 Da / Num. of mol.: 2 / Fragment: Extracellular catalytic domain / Mutation: L266G, G268A
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: AB0, ABO / Plasmid: PCW DELTA 1AC / Production host: Escherichia coli (E. coli) / Strain (production host): BL21
References: UniProt: P16442, glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, fucosylgalactoside 3-alpha-galactosyltransferase

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Non-polymers , 5 types, 549 molecules

#2: Chemical ChemComp-MN / MANGANESE (II) ION


Mass: 54.938 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mn
#3: Chemical ChemComp-5GW / 5-phenyluridine 5'-(trihydrogen diphosphate)


Mass: 480.257 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C15H18N2O12P2
#4: Chemical ChemComp-BHE / octyl 2-O-(6-deoxy-alpha-L-galactopyranosyl)-beta-D-galactopyranoside / H-antigen acceptor / alpha-L-Fucp-(1,2)-Beta-D-Galp-O(CH2)7CH3


Mass: 438.510 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C20H38O10
#5: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: SO4
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 542 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.28 Å3/Da / Density % sol: 46.01 %
Crystal growTemperature: 295 K / Method: vapor diffusion, hanging drop / pH: 7
Details: 50 mM MOPS pH 7, 50-200 mM AMMONIUM SULFATE, 50 mM MnCl2, AND 6-9% PEG-3350 , VAPOR DIFFUSION, HANGING DROP, temperature 295K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: MAX II / Beamline: I911-2 / Wavelength: 1.03796 Å
DetectorType: MAR CCD 165 mm / Detector: CCD / Date: Mar 26, 2010
Details: Multilayer mirror, curved to focus in the vertical (R = 400 m)
RadiationMonochromator: Bent Si (111) crystal, horizontally focusing / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.03796 Å / Relative weight: 1
ReflectionResolution: 1.68→30 Å / Num. all: 73143 / Num. obs: 70045 / % possible obs: 95.8 % / Observed criterion σ(I): -3 / Redundancy: 5.9 % / Biso Wilson estimate: 25.996 Å2 / Rmerge(I) obs: 0.067 / Net I/σ(I): 17.48
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. measured obsNum. unique allNum. unique obs% possible all
1.68-1.724.80.6882.23255225325481090.3
1.72-1.7750.5283.03262005223480992.1
1.77-1.825.30.4054267475074472893.2
1.82-1.885.50.3215.19269104884460294.2
1.88-1.945.70.2546.49272754778453494.9
1.94-2.015.90.1968.54273014623441195.4
2.01-2.086.10.1610.77272884464429596.2
2.08-2.176.20.13513.05268004305414996.4
2.17-2.276.30.10616.49262244131399996.8
2.27-2.386.40.09418.71254863969385697.2
2.38-2.56.50.08520.24244253778367397.2
2.5-2.666.50.0724.1232613581350898
2.66-2.846.50.06325.92218183360330798.4
2.84-3.076.40.05329.75202503152309898.3
3.07-3.366.30.04435.21186032920286298
3.36-3.766.20.0440.08164952661262198.5
3.76-4.346.30.03644.71147342350232498.9
4.34-5.316.20.03246.63125932016198198.3
5.31-7.516.40.03144.48101651596158099
7.51-305.50.02546.38527895389894.2

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Phasing

PhasingMethod: molecular replacement
Phasing MRRfactor: 30.69 / Model details: Phaser MODE: MR_AUTO
Highest resolutionLowest resolution
Rotation2.5 Å29.05 Å
Translation2.5 Å29.05 Å

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Processing

Software
NameVersionClassificationNB
XSCALEdata scaling
PHASER2.1.4phasing
PHENIX1.7.1_743refinement
PDB_EXTRACT3.1data extraction
MAR345data collection
XDSdata reduction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 2RIT

2rit


Resolution: 1.68→29.053 Å / Occupancy max: 1 / Occupancy min: 0.12 / FOM work R set: 0.9009 / SU ML: 0.47 / Isotropic thermal model: Isotropic+tls / Cross valid method: THROUGHOUT / σ(F): 1.99 / Phase error: 16.96 / Stereochemistry target values: MLHL
RfactorNum. reflection% reflectionSelection details
Rfree0.1918 2799 4 %Random
Rwork0.1638 ---
obs0.1649 69982 95.8 %-
Solvent computationShrinkage radii: 0.83 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 42.444 Å2 / ksol: 0.382 e/Å3
Displacement parametersBiso max: 90.26 Å2 / Biso mean: 21.9192 Å2 / Biso min: 7.11 Å2
Baniso -1Baniso -2Baniso -3
1-2.0355 Å2-0 Å20 Å2
2---2.2067 Å20 Å2
3---0.1712 Å2
Refinement stepCycle: LAST / Resolution: 1.68→29.053 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4712 0 129 542 5383
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.015012
X-RAY DIFFRACTIONf_angle_d1.36823
X-RAY DIFFRACTIONf_chiral_restr0.09745
X-RAY DIFFRACTIONf_plane_restr0.007851
X-RAY DIFFRACTIONf_dihedral_angle_d13.341869
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 20

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection allNum. reflection obs% reflection obs (%)
1.68-1.7090.29561290.281131103239323990
1.709-1.740.28221320.24931673299329992
1.74-1.77350.2271340.229731943328332893
1.7735-1.80970.25851340.213232173351335193
1.8097-1.8490.26671360.20732673403340394
1.849-1.8920.24691360.182332693405340594
1.892-1.93940.18041370.162632763413341395
1.9394-1.99180.19321380.15233153453345396
1.9918-2.05040.17271400.148533583498349896
2.0504-2.11650.22281390.154133293468346896
2.1165-2.19220.18521390.157833583497349797
2.1922-2.27990.21291390.152733603499349997
2.2799-2.38360.19521430.15434233566356697
2.3836-2.50920.21861420.156134033545354597
2.5092-2.66630.18581430.153634333576357698
2.6663-2.8720.21451450.159434723617361799
2.872-3.16070.17691450.158934673612361298
3.1607-3.61740.18111460.151935063652365299
3.6174-4.55470.13811470.138235473694369499
4.5547-29.05750.18521550.180737123867386798
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.78610.24840.52091.2971-0.98691.8408-0.1179-0.0392-0.1532-0.03240.26640.3923-0.1282-0.3222-0.09660.24050.00890.03230.1871-0.01040.152522.427414.27769.9169
20.40381.8771-1.16348.9896-5.49943.3823-0.10350.0790.0273-0.66250.38-0.07230.4163-0.1458-0.23660.24830.01790.05710.1910.03820.241542.235615.789114.8821
30.7758-0.255-0.87470.94810.14871.9539-0.00920.0140.06650.0616-0.0084-0.120.0994-0.06350.01140.0793-0.0045-0.00640.12270.01150.097536.24226.396317.0329
43.1963-0.43090.75040.8872-0.27980.88740.04340.087-0.0172-0.0427-0.02130.07170.1409-0.0554-0.02060.1214-0.01180.00770.1225-0.00170.095224.617620.1925.199
52.29932.3433-0.37576.7167-0.71870.8948-0.08320.04380.1009-0.22410.07630.43370.0171-0.09810.02060.0940.0028-0.02470.1008-0.01280.110920.948936.66772.4822
60.98720.034-0.11742.0771-0.16541.4975-0.0230.11250.1108-0.1142-0.0799-0.0802-0.22110.06060.09140.1118-0.0126-0.00350.13170.02590.083230.391341.459-5.5455
70.7898-0.42480.29922.3756-0.04561.2236-0.00230.03440.0415-0.1435-0.08140.1572-0.1695-0.09870.06960.0930.0199-0.01730.1233-0.01410.108220.683140.2988-1.8978
80.1163-0.3776-0.40411.23191.31341.4014-0.09580.13540.2718-0.5191-0.0690.3492-0.1674-0.1567-0.22010.41340.0034-0.11670.13370.0440.222823.923947.278-11.9399
90.85460.4733-0.67111.1448-0.16871.6698-0.1068-0.10570.4894-0.0014-0.01650.1649-0.4475-0.05440.0550.16570.0276-0.07360.135-0.0350.245420.196945.7261-3.3327
101.6638-1.0057-0.20020.771-0.66473.833-0.0244-0.10870.65870.1014-0.1447-0.1614-0.79550.2634-0.26690.15470.0899-0.01490.0478-0.0310.218321.763750.54897.6423
110.38280.03550.35381.24880.24870.8543-0.0264-0.07720.0873-0.07510.0096-0.0488-0.04070.03890.03820.08750.0047-0.00140.09060.01180.090929.481937.90762.0886
120.3111-0.0941-0.06440.4085-0.05380.9224-0.0370.02030.07420.09070.0053-0.209-0.11070.1838-0.00010.0525-0.0048-0.02180.0984-0.00380.103434.842834.348912.3909
132.76-1.91321.24944.2135-1.86273.1268-0.278-0.07270.39380.40460.1316-0.2792-0.47490.19560.51140.1544-0.0803-0.10850.1787-0.01980.183941.284747.803923.5586
140.23170.4292-0.02480.90.0720.40490.0221-0.22280.25040.5632-0.1333-0.3899-0.30240.01510.09780.3025-0.0411-0.07230.1805-0.04290.198736.443647.387527.3392
151.01820.17680.30970.5294-0.20340.8087-0.0644-0.18380.0160.1541-0.0392-0.124-0.14120.03810.06260.0993-0.0152-0.02910.0898-0.00840.118130.839339.297813.1482
166.4543-1.37441.55082.69731.8935.69590.1608-0.5631-0.12220.7332-0.08850.81690.2069-1.0069-0.05680.2041-0.00370.06380.19240.00250.180617.842140.484516.0392
171.49170.85130.73024.85751.81361.9061-0.0554-0.15120.51030.0831-0.16710.3686-0.5473-0.34780.0920.26980.04320.00360.1664-0.04510.216124.06953.116918.3816
181.79210.3223-0.18660.5359-0.09780.93160.0523-0.18060.49350.17090.0076-0.0143-0.48410.0570.20350.2106-0.0455-0.06460.0016-0.0954-0.030331.554445.816319.1119
190.930.503-0.29760.2737-0.13170.6083-0.0214-0.0987-0.0722-0.0430.0506-0.18560.030.11340.07890.0517-0.02060.01750.1390.00740.186743.708234.99895.1192
200.56120.4401-0.12421.48470.3351.5869-0.06470.08830.2873-0.1492-0.01-0.2163-0.09190.279-0.07160.0662-0.02140.03480.12790.03180.188740.381835.12661.2903
210.43340.23590.52021.9966-0.40822.73470.0213-0.18480.0910.0390.04320.3732-0.0157-0.59110.44990.2848-0.0452-0.12080.205-0.01760.118529.25335.46527.2254
221.14312.0534-1.81835.6011-4.89234.2731-0.02480.204-0.04290.5907-0.0606-0.448-0.7076-0.12420.06550.2098-0.012-0.06140.27080.07810.256444.496622.668922.4054
230.7799-0.07860.6450.5344-0.45191.8847-0.0879-0.06540.00810.052-0.0322-0.0576-0.17110.07820.1040.0687-0.0032-0.01230.13160.01470.08433.383617.608420.233
242.8574-0.719-0.95661.43230.1152.0430.0882-0.16210.0928-0.0104-0.02290.1184-0.40990.0349-0.03020.15670.0079-0.01260.1299-0.00550.127.480129.469931.9907
251.85470.89180.06065.4714-0.48871.46290.016-0.11050.1755-0.0154-0.01840.1178-0.1372-0.06790.0390.14650.00850.02220.1321-0.01690.108914.883918.284334.7896
261.58960.5270.2982.2473-0.35971.32920.0661-0.2685-0.20770.2232-0.1419-0.02330.0908-0.07060.06190.1409-0.00740.02060.13380.01520.093819.88388.770542.8185
272.59842.2371-0.42853.5157-0.0730.89180.1099-0.1992-0.04790.1904-0.1810.1933-0.0294-0.11760.07330.1392-0.01040.04280.15090.00390.120912.537115.379939.0771
280.74490.029-0.32840.0014-0.01370.14560.3352-0.53630.26010.4996-0.45610.3469-0.0365-0.1227-0.27310.2564-0.10040.09580.1993-0.02660.183111.45897.830149.2637
290.3857-0.58690.18081.0251-0.5790.77730.00260.2645-0.3852-0.0489-0.09570.47580.3583-0.2595-0.06350.2305-0.05440.0680.1583-0.09720.27049.157911.056340.607
301.94480.53740.34160.2092-0.31052.7591-0.08390.0603-0.75490.24060.07270.61660.7352-0.12110.03620.2550.00480.04040.20390.01320.30948.49615.153829.563
310.3970.261-0.31170.72290.04640.8050.0732-0.0408-0.07160.0867-0.06810.04760.07990.0249-0.00040.10680.01180.0110.13110.01160.134221.125212.242935.1243
321.2250.03030.3470.6892-0.2351.4161-0.04070.0084-0.16480.06190.033-0.06060.1310.13340.01380.0878-0.00290.00240.1050.00640.085827.580111.95324.8612
331.34960.50060.60722.52041.18293.019-0.06140.1705-0.3837-0.26440.081-0.29060.54670.1669-0.13830.27710.01440.04460.1305-0.03610.204724.267-2.779813.7175
342.41641.93160.0922.6836-0.44820.2447-0.11820.2496-0.4385-0.39070.0016-0.22670.2696-0.04630.00890.2273-0.03190.02390.1649-0.06520.152721.29810.44059.8361
350.9148-0.1225-0.42941.01360.22911.30330.02320.0016-0.09130.07-0.08260.05610.0928-0.13140.05550.1147-0.0140.0080.10710.00370.099621.48910.286624.0619
363.153-1.3963-1.1695.034-0.58522.75150.01880.23040.3334-0.54460.04510.7283-0.2929-0.9707-0.12540.12480.0152-0.04020.2017-0.00460.209810.334416.711921.2555
371.16211.85531.14924.78052.09751.17530.02060.0423-0.25730.0698-0.1060.45710.3027-0.52130.10250.1584-0.0982-0.01150.2439-0.00910.20728.04562.772118.8171
381.3190.10820.28751.39940.00530.4861-0.04060.0352-0.262-0.03570.02170.09350.2908-0.1962-0.00140.1643-0.04950.02240.1182-0.0130.118.32944.508618.0715
391.445-0.66250.61161.79180.13690.4147-0.07340.0395-0.16110.13750.0998-0.18430.04390.0768-0.0270.13440.02270.00090.12660.00410.140334.35276.692431.9768
402.0356-0.73280.59120.67150.64052.5933-0.08910.0813-0.2452-0.03010.0306-0.15280.26130.21820.04690.13720.0374-0.00310.12760.02710.172131.63168.188635.9731
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1(chain A and resid 58:76)A58 - 76
2X-RAY DIFFRACTION2(chain A and resid 77:82)A77 - 82
3X-RAY DIFFRACTION3(chain A and resid 83:98)A83 - 98
4X-RAY DIFFRACTION4(chain A and resid 99:111)A99 - 111
5X-RAY DIFFRACTION5(chain A and resid 112:122)A112 - 122
6X-RAY DIFFRACTION6(chain A and resid 123:143)A123 - 143
7X-RAY DIFFRACTION7(chain A and resid 144:154)A144 - 154
8X-RAY DIFFRACTION8(chain A and resid 155:163)A155 - 163
9X-RAY DIFFRACTION9(chain A and resid 164:178)A164 - 178
10X-RAY DIFFRACTION10(chain A and resid 179:203)A179 - 203
11X-RAY DIFFRACTION11(chain A and resid 204:218)A204 - 218
12X-RAY DIFFRACTION12(chain A and resid 219:241)A219 - 241
13X-RAY DIFFRACTION13(chain A and resid 242:250)A242 - 250
14X-RAY DIFFRACTION14(chain A and resid 251:262)A251 - 262
15X-RAY DIFFRACTION15(chain A and resid 263:274)A263 - 274
16X-RAY DIFFRACTION16(chain A and resid 275:279)A275 - 279
17X-RAY DIFFRACTION17(chain A and resid 280:293)A280 - 293
18X-RAY DIFFRACTION18(chain A and resid 294:318)A294 - 318
19X-RAY DIFFRACTION19(chain A and resid 319:335)A319 - 335
20X-RAY DIFFRACTION20(chain A and resid 336:346)A336 - 346
21X-RAY DIFFRACTION21(chain B and resid 58:76)B58 - 76
22X-RAY DIFFRACTION22(chain B and resid 77:82)B77 - 82
23X-RAY DIFFRACTION23(chain B and resid 83:98)B83 - 98
24X-RAY DIFFRACTION24(chain B and resid 99:111)B99 - 111
25X-RAY DIFFRACTION25(chain B and resid 112:122)B112 - 122
26X-RAY DIFFRACTION26(chain B and resid 123:143)B123 - 143
27X-RAY DIFFRACTION27(chain B and resid 144:154)B144 - 154
28X-RAY DIFFRACTION28(chain B and resid 155:163)B155 - 163
29X-RAY DIFFRACTION29(chain B and resid 164:178)B164 - 178
30X-RAY DIFFRACTION30(chain B and resid 179:203)B179 - 203
31X-RAY DIFFRACTION31(chain B and resid 204:218)B204 - 218
32X-RAY DIFFRACTION32(chain B and resid 219:241)B219 - 241
33X-RAY DIFFRACTION33(chain B and resid 242:250)B242 - 250
34X-RAY DIFFRACTION34(chain B and resid 251:262)B251 - 262
35X-RAY DIFFRACTION35(chain B and resid 263:274)B263 - 274
36X-RAY DIFFRACTION36(chain B and resid 275:279)B275 - 279
37X-RAY DIFFRACTION37(chain B and resid 280:293)B280 - 293
38X-RAY DIFFRACTION38(chain B and resid 294:318)B294 - 318
39X-RAY DIFFRACTION39(chain B and resid 319:335)B319 - 335
40X-RAY DIFFRACTION40(chain B and resid 336:346)B336 - 346

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