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- PDB-3um1: Crystal structure of the Brox Bro1 domain in complex with the C-t... -
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Basic information
Entry | Database: PDB / ID: 3um1 | ||||||
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Title | Crystal structure of the Brox Bro1 domain in complex with the C-terminal tail of CHMP5 | ||||||
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![]() | MEMBRANE PROTEIN/TRANSPORT PROTEIN / beta hairpin / ESCRT-III / CHMPs / MEMBRANE PROTEIN-TRANSPORT PROTEIN complex / BROX | ||||||
Function / homology | ![]() amphisome membrane / multivesicular body-lysosome fusion / viral budding / vesicle fusion with vacuole / ESCRT III complex disassembly / late endosome to lysosome transport / ESCRT III complex / kinetochore microtubule / late endosome to vacuole transport via multivesicular body sorting pathway / mitotic nuclear membrane reassembly ...amphisome membrane / multivesicular body-lysosome fusion / viral budding / vesicle fusion with vacuole / ESCRT III complex disassembly / late endosome to lysosome transport / ESCRT III complex / kinetochore microtubule / late endosome to vacuole transport via multivesicular body sorting pathway / mitotic nuclear membrane reassembly / regulation of centrosome duplication / nuclear membrane reassembly / cellular response to muramyl dipeptide / midbody abscission / multivesicular body sorting pathway / vesicle budding from membrane / plasma membrane repair / membrane fission / multivesicular body membrane / ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway / multivesicular body assembly / regulation of mitotic spindle assembly / mitotic metaphase chromosome alignment / nucleus organization / viral budding via host ESCRT complex / autophagosome membrane / autophagosome maturation / regulation of receptor recycling / nuclear pore / Endosomal Sorting Complex Required For Transport (ESCRT) / multivesicular body / viral budding from plasma membrane / erythrocyte differentiation / Budding and maturation of HIV virion / kinetochore / autophagy / protein transport / nuclear envelope / midbody / cellular response to lipopolysaccharide / nuclear membrane / cadherin binding / lysosomal membrane / extracellular exosome / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Jiang, J.S. / Mu, R.L. / Xiao, T. | ||||||
![]() | ![]() Title: Two Distinct Binding Modes Define the Interaction of Brox with the C-Terminal Tails of CHMP5 and CHMP4B. Authors: Mu, R. / Dussupt, V. / Jiang, J. / Sette, P. / Rudd, V. / Chuenchor, W. / Bello, N.F. / Bouamr, F. / Xiao, T.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 168.6 KB | Display | ![]() |
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PDB format | ![]() | 132.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 3ulyC ![]() 3um0C ![]() 3um2C ![]() 3um3C ![]() 3r9mS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 42533.410 Da / Num. of mol.: 2 / Fragment: Brox bro1 domain 2-377 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein | Mass: 7294.708 Da / Num. of mol.: 2 / Fragment: C-terminal tail of CHMP5 151-219 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: C9orf83, CGI-34, CHMP5, HSPC177, PNAS-114, PNAS-2, SNF7DC2 Plasmid: pET30a / Production host: ![]() ![]() #3: Chemical | ChemComp-GOL / | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.24 Å3/Da / Density % sol: 45.07 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 20% PEG 8000, 0.1M sodium cacodylate, 0.2M magnesium acetate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Sep 3, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.075 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→50 Å / Num. obs: 23032 / % possible obs: 91.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.3 % / Biso Wilson estimate: 38.1 Å2 / Rmerge(I) obs: 0.133 / Net I/σ(I): 7.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 3R9M Resolution: 2.708→43.319 Å / SU ML: 0.36 / Cross valid method: THROUGHOUT / σ(F): 0 / Phase error: 25.45 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.95 Å / VDW probe radii: 1.2 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 32.133 Å2 / ksol: 0.342 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 41.9 Å2
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Refinement step | Cycle: LAST / Resolution: 2.708→43.319 Å
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Refine LS restraints |
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LS refinement shell |
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