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- PDB-3um3: Crystal structure of the Brox Bro1 domain in complex with the C-t... -
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Basic information
Entry | Database: PDB / ID: 3um3 | ||||||
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Title | Crystal structure of the Brox Bro1 domain in complex with the C-terminal tail of CHMP4B | ||||||
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![]() | MEMBRANE PROTEIN/TRANSPORT PROTEIN / alpha-helix of C-terminal tail of CHMP4B / ESCRT-III / CHMPs / MEMBRANE PROTEIN-TRANSPORT PROTEIN complex / BROX | ||||||
Function / homology | ![]() ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway / maintenance of lens transparency / amphisome membrane / multivesicular body-lysosome fusion / viral budding / vesicle fusion with vacuole / late endosome to lysosome transport / ESCRT III complex / kinetochore microtubule / late endosome to vacuole transport via multivesicular body sorting pathway ...ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway / maintenance of lens transparency / amphisome membrane / multivesicular body-lysosome fusion / viral budding / vesicle fusion with vacuole / late endosome to lysosome transport / ESCRT III complex / kinetochore microtubule / late endosome to vacuole transport via multivesicular body sorting pathway / mitotic nuclear membrane reassembly / regulation of centrosome duplication / nuclear membrane reassembly / Sealing of the nuclear envelope (NE) by ESCRT-III / midbody abscission / post-translational protein targeting to endoplasmic reticulum membrane / multivesicular body sorting pathway / membrane coat / vesicle budding from membrane / plasma membrane repair / membrane fission / multivesicular body membrane / ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway / multivesicular body assembly / regulation of mitotic spindle assembly / nervous system process / Translation of Replicase and Assembly of the Replication Transcription Complex / exit from mitosis / mitotic metaphase chromosome alignment / Macroautophagy / nucleus organization / viral budding via host ESCRT complex / autophagosome membrane / autophagosome maturation / mitotic cytokinesis / protein polymerization / Pyroptosis / nuclear pore / Endosomal Sorting Complex Required For Transport (ESCRT) / multivesicular body / viral budding from plasma membrane / HCMV Late Events / regulation of autophagy / macroautophagy / Late endosomal microautophagy / Budding and maturation of HIV virion / cytoplasmic side of plasma membrane / kinetochore / autophagy / protein transport / nuclear envelope / Translation of Replicase and Assembly of the Replication Transcription Complex / midbody / nuclear membrane / vesicle / endosome / cadherin binding / lysosomal membrane / protein homodimerization activity / extracellular exosome / identical protein binding / nucleus / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Jiang, J.S. / Mu, R.L. / Xiao, T. | ||||||
![]() | ![]() Title: Two Distinct Binding Modes Define the Interaction of Brox with the C-Terminal Tails of CHMP5 and CHMP4B. Authors: Mu, R. / Dussupt, V. / Jiang, J. / Sette, P. / Rudd, V. / Chuenchor, W. / Bello, N.F. / Bouamr, F. / Xiao, T.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 85.2 KB | Display | ![]() |
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PDB format | ![]() | 64.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 3ulyC ![]() 3um0C ![]() 3um1C ![]() 3um2C ![]() 3r9mS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 46521.863 Da / Num. of mol.: 1 / Fragment: Brox bro1 domain 2-377 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Protein | Mass: 11699.792 Da / Num. of mol.: 1 / Fragment: C-terminal tail of CHMP4B 121-224 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal grow | Temperature: 273 K / Method: vapor diffusion, hanging drop / pH: 7.2 Details: 1.0M sodium citrate, 0.1M imidazol, 15% glycerol, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Apr 16, 2010 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.075 Å / Relative weight: 1 |
Reflection | Resolution: 3.8→50 Å / Num. obs: 21693 / % possible obs: 99.3 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.3 % / Biso Wilson estimate: 145 Å2 / Rmerge(I) obs: 0.036 / Net I/σ(I): 18.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 3R9M Resolution: 3.8→48.76 Å / Rfactor Rfree error: 0.005 / Data cutoff high absF: 5336689.33 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber / Details: BULK SOLVENT MODEL USED
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 42.2265 Å2 / ksol: 0.33 e/Å3 | |||||||||||||||||||||||||
Displacement parameters | Biso mean: 83.3 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3.8→48.76 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: NONE | |||||||||||||||||||||||||
LS refinement shell | Resolution: 3.8→4.04 Å / Rfactor Rfree error: 0.019 / Total num. of bins used: 6
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Xplor file |
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