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- PDB-3thz: Human MutSbeta complexed with an IDL of 6 bases (Loop6) and ADP -

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Basic information

Entry
Database: PDB / ID: 3thz
TitleHuman MutSbeta complexed with an IDL of 6 bases (Loop6) and ADP
Components
  • DNA Loop6 minus strand
  • DNA Loop6 plus strand
  • DNA mismatch repair protein Msh2
  • DNA mismatch repair protein Msh3
KeywordsDNA BINDING PROTEIN/DNA / ABC family ATPase / mismatch recognition / mismatched unpaired IDL DNA / DNA BINDING PROTEIN-DNA complex
Function / homology
Function and homology information


somatic recombination of immunoglobulin genes involved in immune response / MutSbeta complex / Defective Mismatch Repair Associated With MSH3 / MutSalpha complex / Defective Mismatch Repair Associated With MSH2 / Defective Mismatch Repair Associated With MSH6 / guanine/thymine mispair binding / somatic recombination of immunoglobulin gene segments / B cell mediated immunity / maintenance of DNA repeat elements ...somatic recombination of immunoglobulin genes involved in immune response / MutSbeta complex / Defective Mismatch Repair Associated With MSH3 / MutSalpha complex / Defective Mismatch Repair Associated With MSH2 / Defective Mismatch Repair Associated With MSH6 / guanine/thymine mispair binding / somatic recombination of immunoglobulin gene segments / B cell mediated immunity / maintenance of DNA repeat elements / positive regulation of helicase activity / positive regulation of isotype switching to IgA isotypes / centromeric DNA binding / mitotic recombination / positive regulation of isotype switching to IgG isotypes / mismatched DNA binding / negative regulation of DNA recombination / Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta) / Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha) / isotype switching / oxidative phosphorylation / response to UV-B / postreplication repair / mitotic intra-S DNA damage checkpoint signaling / ATP-dependent DNA damage sensor activity / germ cell development / response to X-ray / intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / somatic hypermutation of immunoglobulin genes / mismatch repair / ATP-dependent activity, acting on DNA / protein localization to chromatin / B cell differentiation / determination of adult lifespan / TP53 Regulates Transcription of DNA Repair Genes / male gonad development / double-strand break repair / double-stranded DNA binding / in utero embryonic development / negative regulation of neuron apoptotic process / damaged DNA binding / chromosome, telomeric region / DNA repair / chromatin binding / enzyme binding / protein homodimerization activity / ATP hydrolysis activity / DNA binding / nucleoplasm / ATP binding / membrane / nucleus
Similarity search - Function
DNA mismatch repair Msh2-type / DNA mismatch repair protein Msh2 / DNA mismatch repair protein MutS-like, N-terminal / DNA mismatch repair protein MutS, connector domain / DNA mismatch repair protein MutS, clamp / DNA mismatch repair protein MutS, N-terminal / MutS, connector domain superfamily / MutS domain I / MutS domain II / MutS family domain IV ...DNA mismatch repair Msh2-type / DNA mismatch repair protein Msh2 / DNA mismatch repair protein MutS-like, N-terminal / DNA mismatch repair protein MutS, connector domain / DNA mismatch repair protein MutS, clamp / DNA mismatch repair protein MutS, N-terminal / MutS, connector domain superfamily / MutS domain I / MutS domain II / MutS family domain IV / MutS domain III / DNA mismatch repair MutS family / DNA mismatch repair protein MutS, C-terminal / DNA mismatch repair protein MutS, core / DNA mismatch repair protein MutS, core domain superfamily / MutS domain V / DNA mismatch repair proteins mutS family signature. / DNA-binding domain of DNA mismatch repair MUTS family / ATPase domain of DNA mismatch repair MUTS family / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
ADENOSINE-5'-DIPHOSPHATE / : / DNA / DNA (> 10) / DNA mismatch repair protein Msh3 / DNA mismatch repair protein Msh2
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 4.3 Å
AuthorsYang, W.
CitationJournal: Nat.Struct.Mol.Biol. / Year: 2012
Title: Mechanism of mismatch repair revealed by human MutS bound to unpaired DNA loops
Authors: Gupta, S. / Gellert, M. / Yang, W.
History
DepositionAug 19, 2011Deposition site: RCSB / Processing site: RCSB
Revision 1.0Dec 21, 2011Provider: repository / Type: Initial release
Revision 1.1Jun 26, 2013Group: Database references
Revision 1.2Jul 17, 2019Group: Data collection / Database references / Refinement description
Category: software / struct_ref_seq
Item: _software.classification / _software.name ..._software.classification / _software.name / _software.version / _struct_ref_seq.db_align_beg / _struct_ref_seq.db_align_end
Revision 1.3Feb 28, 2024Group: Data collection / Database references / Derived calculations
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / struct_ref_seq_dif / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: DNA mismatch repair protein Msh2
B: DNA mismatch repair protein Msh3
D: DNA Loop6 minus strand
E: DNA Loop6 plus strand
hetero molecules


Theoretical massNumber of molelcules
Total (without water)225,4835
Polymers225,0564
Non-polymers4271
Water00
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area14330 Å2
ΔGint-115 kcal/mol
Surface area82270 Å2
MethodPISA
Unit cell
Length a, b, c (Å)103.680, 154.630, 161.590
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

#1: Protein DNA mismatch repair protein Msh2 / hMSH2 / MutS protein homolog 2


Mass: 104861.875 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: MSH2 / Cell line (production host): Hi5 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P43246
#2: Protein DNA mismatch repair protein Msh3 / hMSH3 / Divergent upstream protein / DUP / Mismatch repair protein 1 / MRP1


Mass: 104289.664 Da / Num. of mol.: 1 / Fragment: UNP residues 219- 1134
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: DUC1, DUG, MSH3 / Cell line (production host): Hi5 / Production host: Spodoptera frugiperda (fall armyworm) / References: GenBank: 119616268, UniProt: P20585*PLUS
#3: DNA chain DNA Loop6 minus strand


Mass: 7329.742 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: chemically synthesized
#4: DNA chain DNA Loop6 plus strand


Mass: 8574.543 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: chemically synthesized
#5: Chemical ChemComp-ADP / ADENOSINE-5'-DIPHOSPHATE


Mass: 427.201 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C10H15N5O10P2 / Comment: ADP, energy-carrying molecule*YM

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.88 Å3/Da / Density % sol: 57.26 %
Crystal growTemperature: 277 K / Method: vapor diffusion, hanging drop
Details: 0.2M Ammonium Acetate, 20-25% PEG3350 (w/v) and 0.1M MES pH 6.5-7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
PH range: 6.5-7.5

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 23-ID-B / Wavelength: 1 Å
DetectorType: MAR scanner 300 mm plate / Detector: IMAGE PLATE / Date: Aug 3, 2009
RadiationMonochromator: MIRROR / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 4.3→50 Å / Num. all: 17782 / Num. obs: 17033 / % possible obs: 96 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 4 % / Rmerge(I) obs: 0.102 / Net I/σ(I): 12.2
Reflection shellResolution: 4.3→4.37 Å / Redundancy: 4 % / Rmerge(I) obs: 0.687 / Mean I/σ(I) obs: 1.8 / % possible all: 92.8

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Processing

Software
NameVersionClassification
PHENIX(phenix.refine: 1.6.4_486)refinement
CNSrefinement
Blu-Icedata collection
DENZOdata reduction
SCALEPACKdata scaling
CNSphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 4.3→49.18 Å / SU ML: 0.81 / σ(F): 0 / Phase error: 31.89 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.285 999 5.92 %
Rwork0.239 --
obs0.241 16879 89.9 %
Solvent computationShrinkage radii: 0.83 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 239.6 Å2 / ksol: 0.32 e/Å3
Displacement parameters
Baniso -1Baniso -2Baniso -3
1--84.0966 Å20 Å2-0 Å2
2--108.0683 Å20 Å2
3----23.9718 Å2
Refinement stepCycle: LAST / Resolution: 4.3→49.18 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms13636 962 27 0 14625
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.01315664
X-RAY DIFFRACTIONf_angle_d0.99420584
X-RAY DIFFRACTIONf_dihedral_angle_d17.6135687
X-RAY DIFFRACTIONf_chiral_restr0.0622369
X-RAY DIFFRACTIONf_plane_restr0.0042461
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
4.259-4.48340.42721360.3761823X-RAY DIFFRACTION74
4.4834-4.76410.33141330.30512079X-RAY DIFFRACTION84
4.7641-5.13160.30531350.25472241X-RAY DIFFRACTION90
5.1316-5.64730.3051310.26922343X-RAY DIFFRACTION93
5.6473-6.46290.32361400.30992405X-RAY DIFFRACTION95
6.4629-8.13660.3061680.23082450X-RAY DIFFRACTION97
8.1366-49.17980.23031560.18692539X-RAY DIFFRACTION95
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.19411.9964-1.01554.6991-2.21983.671-0.0273-0.70320.0425-0.8018-0.0769-0.01040.82341.7626-0.01982.30010.44960.01362.3923-0.18612.179217.8814-3.7735-49.7262
24.5147-3.36691.43062.09961.4583.6693-0.0512-1.28070.15460.3824-0.1018-1.0111-0.35971.61420.11032.2330.2219-0.07962.4331-0.15551.86879.5974-6.2176-23.836
33.64880.90370.03893.4184-0.1812.8177-0.2858-0.9629-0.24520.09870.39060.08090.7261-0.1813-0.08842.22230.2832-0.21272.97060.02662.1463-0.4514-14.5719-24.0728
40.48330.4463.01950.386-0.82071.22390.853-0.9153-0.7818-1.3889-3.1873-0.6767-0.61272.9562-0.23322.58-0.1525-0.29973.39110.81682.778421.1687-11.8178-11.1113
51.1822-1.4195-0.33543.1409-3.2889-0.48530.4497-0.92940.2113-0.2623-0.775-0.4572-1.27131.20180.23482.36120.1708-0.21913.5182-0.37871.838215.5034-9.2591-17.693
60.72143.12941.20140.73641.4403-0.7629-0.21-1.2611.69760.33470.17530.4977-0.70010.6262-0.02552.58240.07910.07252.6464-0.68583.0392-3.174511.7427-15.3015
70.3113-0.1172-0.3237-0.6857-2.351.1202-0.1059-2.2712.02250.55020.5628-0.6418-0.16213.30790.09562.9299-0.3139-0.27723.4336-0.89413.134115.497720.1911-13.8693
80.51550.04930.51390.2750.85510.79552.2762-2.79523.58032.22191.5802-1.3998-1.17182.58780.46853.1838-0.630.17584.1545-1.27674.688728.743224.6341-18.5647
90.2161-0.58430.27870.8235-0.46710.2901-1.454-2.4254-0.4027-0.3102-2.9168-1.0784-2.0711.34840.30713.6534-0.4947-0.43022.72070.49054.482245.18827.2238-22.6373
100.0591-0.17810.7062-1.180.1691-0.1720.048-0.2958-0.10350.4316-2.3541-0.96430.5183-0.0974-0.08583.07250.03340.11844.5647-0.41914.089453.984736.2889-50.0096
111.48280.98690.15560.5421-0.93531.3625-1.0748-0.44940.64820.2944-1.58950.0559-0.1221-1.9343-0.10483.07940.40540.03982.589-0.1994.212748.360822.9239-33.4115
121.42271.1349-1.27240.275-0.7624-0.4420.2124-0.79710.10910.53610.1234-0.568-0.901-0.04380.02012.840.0345-0.0193.3276-0.58132.7401-1.972914.2651-13.1159
130.86520.1234-0.17450.56190.46940.405-0.0367-2.3072-0.79351.88482.7474-0.8354-0.41720.04910.05393.7198-0.2301-0.32432.13950.39092.8819-21.224719.8622-29.1272
143.21452.8393-0.39935.48640.2642.2481-0.0082-1.48250.93360.19090.2501-0.1164-0.5952-0.59980.04542.24110.50330.09732.8889-0.64662.1331-20.67680.8764-17.0719
150.5229-0.1107-0.12381.0845-0.2320.5072-0.5938-2.21092.3081-1.59623.91251.19391.0334-1.71710.1782.00440.4371-0.66513.09970.1892.835-32.2797-12.2359-27.6634
161.16473.21952.42125.44241.58571.25930.4721-0.64290.7608-0.06260.46471.7808-0.5680.2902-0.08732.29430.65140.33912.7807-0.22832.6452-34.08640.9004-19.5378
172.05751.4361-0.21851.32661.31820.55090.52910.22210.6340.3676-0.33540.3522-1.4533-0.1060.04162.42990.22930.03022.4896-0.34012.8921-48.09980.0992-31.1886
182.23251.6234-0.3393.19491.20080.9740.3583-1.7994-0.5096-2.8161-3.15472.90161.14861.42370.14832.53040.05790.10483.82720.85193.4291-67.2553-18.7217-25.3995
19-0.02690.3701-0.05444.74690.89030.2016-2.9656-0.81111.9658-1.84020.00011.88872.59870.37960.0412.19460.3482-0.3523.5191-0.12976.0227-79.4045-20.6097-29.9489
200.8272-0.73460.26761.23251.96741.0603-0.8846-1.65621.06220.2053-0.5367-0.6884-1.20780.3068-0.27662.52490.9487-0.2093.3599-0.30543.7794-66.1909-5.1552-27.8215
212.17410.99142.29341.25011.47591.83320.3961-0.86690.6564-0.4513-0.4052-1.1793-1.57310.2386-0.04592.8991-0.18190.2712.2766-0.42592.719312.513625.1433-52.8053
22-0.39492.76951.548-0.3592-0.38092.2309-0.0384-0.06611.457-0.86771.11121.0928-0.9141.09530.00972.7476-0.00220.46232.5947-0.54312.876811.674426.0532-65.6964
23-0.193-1.0920.3182-0.03270.5732-0.46231.18323.30530.31341.0284-0.0177-0.07672.43032.0793-0.0123.57960.3374-0.24672.0156-0.59953.00693.15012.1607-63.7696
240.45672.2781-0.2620.27983.3256-0.44542.2457-0.63720.1748-1.8378-1.7014-0.0108-2.241-0.00680.02332.99570.32710.19981.91960.16152.2743-21.135115.337-61.9133
251.1613-0.6467-1.41320.0436-0.76762.50271.0055-1.3471.28190.57130.45140.9594-0.2104-0.3780.08752.60310.4860.2651.8854-0.04842.4133-17.282213.223-51.8908
260.87360.4047-0.25583.3525-0.98861.5709-0.19530.51341.62270.79410.33570.9145-0.6109-1.17240.05172.83320.35040.30111.68990.352.7829-16.294124.8833-68.5205
272.51253.6697-3.34483.4988-1.3348-0.4305-0.05090.34250.3338-0.0998-0.115-0.168-0.39560.0128-0.02142.45730.14850.07911.6039-0.17872.1305-14.3791-1.3335-61.1931
280.67940.0095-0.25370.6703-0.24670.58780.60716.06471.6733-0.62530.5719-0.5383-2.3688-1.9811-0.15182.88570.0064-0.17732.22920.63572.5102-21.4083-5.4849-69.2661
291.3315-0.49360.74010.2765-0.75830.8322-1.8454-0.5748-1.7141-0.23521.1277-0.21961.1038-1.632-0.11632.6675-0.0450.26932.89210.41622.8452-8.0651-16.4772-75.9461
301.481-0.03370.4014-0.06270.07330.9489-1.302-0.19760.8887-1.69192.058-0.25580.6691-0.60070.02482.94280.05370.38231.7594-0.65092.14543.56438.7927-74.2812
31-0.4566-0.2074-1.84621.619-1.9475-0.5655-1.0241.8307-0.9074-2.09351.4264-1.47230.301-0.4347-0.03442.8278-0.2471-0.11962.8252-0.0072.4343-1.3788-5.539-85.3826
320.9597-0.1095-0.93360.7816-0.50220.0484-0.54280.45130.6788-2.1507-0.5178-0.06750.5796-0.28540.00153.66310.03530.50912.50030.4072.375317.084315.2482-86.7581
331.08962.2914-3.35531.7429-1.47752.17410.0921.35510.00640.2020.04710.1997-0.26520.45990.18943.66640.61580.13752.9336-0.27513.223744.279126.8979-81.396
340.6782-0.28970.95832.49580.73290.2682-0.45791.16040.4161-0.08730.6688-0.3816-0.28031.3540.1342.8329-0.11520.30172.9378-0.30952.696326.667326.255-79.2402
35-0.2327-0.2999-0.73711.1275-0.9613.3385-0.44580.94630.6338-0.21990.03690.63290.4918-1.2634-0.03932.53750.25620.20522.0887-0.12962.3882-8.8641-7.3785-73.3248
363.92456.2114-1.27883.0589-1.18141.3716-0.39130.1333-0.4143-0.52670.645-0.34950.5188-1.1166-0.08032.06550.133-0.13971.510.13012.1339-25.348-15.7384-53.9403
371.1275-0.757-0.43662.23190.66271.2049-0.42190.0261.29790.47691.04471.9761-0.46161.2235-0.09422.29140.1002-0.1472.00890.11232.468-33.3184-1.8459-49.162
380.31550.15220.85551.77431.07771.4979-0.4406-0.19920.23680.80180.0870.93630.6739-0.7185-0.0832.11540.0019-0.17672.63240.04652.5544-41.3745-9.6725-43.9044
390.69180.0607-1.14070.48160.92641.250.3338-1.83211.8866-0.12251.05260.77941.3917-1.6682-0.03332.50180.15240.0181.94250.2492.4867-29.9712-23.9561-40.4356
402.06933.2367-2.79813.65961.71764.0386-0.4186-2.54690.1551-0.89540.0321-0.42580.6743-2.3026-0.12632.1890.03570.34113.08430.14042.0624-48.1699-17.6619-29.1831
411.8618-0.35181.055-0.0403-0.22210.9402-1.73972.35541.7689-0.8163-2.9259-1.3965-1.90721.0423-0.18034.490.79790.425.520.05426.058859.581816.8782-67.1276
42-0.0179-0.04120.01471.447-0.15880.0107-0.7749-1.67890.2355-3.2937-1.8966-2.15770.8855-0.6915-0.58484.53120.2727-0.77966.81330.85113.601846.05847.3754-60.8648
430.80370.71512.38961.4351-0.58181.95271.90290.4517-0.5616-2.46092.02141.57172.46015.981-0.08018.8278-5.37091.6169.8999-0.42423.97732.793318.291-60.8906
441.99472.2197-7.68322.11052.69072.0352-0.7073-1.85894.62534.51590.5509-1.0913.28861.544-1.08954.1136-0.83710.1193.2044-0.85892.660526.29932.9133-57.3093
451.6455-0.36870.32295.7314-5.77138.3003-0.7867-5.5347-0.9547-0.25945.07910.6236-3.2295-2.5293-0.11163.1231-0.9886-0.12643.48660.21552.449938.034343.9364-56.2817
461.5138-5.4838-1.64982.0238-7.18921.52041.21874.88750.47141.0784-1.2179-2.57857.7077-1.87511.82377.6939-3.74941.31614.6118-1.08783.030432.856742.3588-62.8477
472.06268.75-5.18491.9873-5.93162.0518-4.38682.53060.38871.7188-0.5688-1.0684-4.78331.01022.84623.5873-0.6772-0.39612.1405-0.3945.317832.855327.816-53.8227
481.1543-1.37690.90132.1704-0.58360.33510.3601-1.0257-0.26360.8570.2911-0.93180.60130.6411-0.50383.04770.2768-0.26033.3803-0.59383.277624.298311.8524-53.2037
490.03240.00120.11020.2684-0.49291.19642.8278-1.1031-0.87220.81940.81852.18267.669-0.71170.10634.2630.5535-0.32723.7889-0.36194.188132.437710.2603-66.6738
507.6905-2.158-1.67282.0946-1.06272.43564.08340.6348-2.8167-2.0168-0.0944-4.98412.46320.91440.41793.56740.5141-0.75976.99050.72094.346850.818414.1522-58.8403
510.2331-0.174-0.50.38670.51170.9360.01010.7261-0.5904-1.10080.49981.15762.8357-0.4918-0.0713.20270.3091-0.31572.4015-0.29472.9-25.72510.2136-27.6883
522.71460.2042-5.30330.0639-0.20721.978-3.9659-2.85981.2438-0.9405-0.21311.88331.1021-3.845-0.06837.43034.83865.983814.15549.685413.082865.017711.5284-63.2668
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1(chain A and resid 11:117)
2X-RAY DIFFRACTION2(chain A and resid 118:175)
3X-RAY DIFFRACTION3(chain A and resid 176:231)
4X-RAY DIFFRACTION4(chain A and resid 232:260)
5X-RAY DIFFRACTION5(chain A and resid 261:295)
6X-RAY DIFFRACTION6(chain A and resid 296:380)
7X-RAY DIFFRACTION7(chain A and resid 381:441)
8X-RAY DIFFRACTION8(chain A and resid 442:470)
9X-RAY DIFFRACTION9(chain A and resid 471:482)
10X-RAY DIFFRACTION10(chain A and resid 483:554)
11X-RAY DIFFRACTION11(chain A and resid 555:580)
12X-RAY DIFFRACTION12(chain A and resid 581:645)
13X-RAY DIFFRACTION13(chain A and resid 646:650)
14X-RAY DIFFRACTION14(chain A and resid 651:749)
15X-RAY DIFFRACTION15(chain A and resid 750:763)
16X-RAY DIFFRACTION16(chain A and resid 764:825)
17X-RAY DIFFRACTION17(chain A and resid 826:882)
18X-RAY DIFFRACTION18(chain A and resid 883:897)
19X-RAY DIFFRACTION19(chain A and resid 898:907)
20X-RAY DIFFRACTION20(chain A and resid 908:930)
21X-RAY DIFFRACTION21(chain B and resid 226:301)
22X-RAY DIFFRACTION22(chain B and resid 302:346)
23X-RAY DIFFRACTION23(chain B and resid 347:367)
24X-RAY DIFFRACTION24(chain B and resid 368:405)
25X-RAY DIFFRACTION25(chain B and resid 406:455)
26X-RAY DIFFRACTION26(chain B and resid 456:519)
27X-RAY DIFFRACTION27(chain B and resid 520:577)
28X-RAY DIFFRACTION28(chain B and resid 578:588)
29X-RAY DIFFRACTION29(chain B and resid 589:616)
30X-RAY DIFFRACTION30(chain B and resid 617:638)
31X-RAY DIFFRACTION31(chain B and resid 639:686)
32X-RAY DIFFRACTION32(chain B and resid 687:715)
33X-RAY DIFFRACTION33(chain B and resid 716:770)
34X-RAY DIFFRACTION34(chain B and resid 771:814)
35X-RAY DIFFRACTION35(chain B and resid 815:849)
36X-RAY DIFFRACTION36(chain B and resid 850:953)
37X-RAY DIFFRACTION37(chain B and resid 954:979)
38X-RAY DIFFRACTION38(chain B and resid 980:1022)
39X-RAY DIFFRACTION39(chain B and resid 1023:1070)
40X-RAY DIFFRACTION40(chain B and resid 1071:1129)
41X-RAY DIFFRACTION41(chain D and resid 3:7)
42X-RAY DIFFRACTION42(chain D and resid 8:11)
43X-RAY DIFFRACTION43(chain D and resid 12:15)
44X-RAY DIFFRACTION44(chain D and resid 16:19)
45X-RAY DIFFRACTION45(chain D and resid 20:24)
46X-RAY DIFFRACTION46(chain D and resid 26:31)
47X-RAY DIFFRACTION47(chain D and resid 32:35)
48X-RAY DIFFRACTION48(chain D and resid 36:40)
49X-RAY DIFFRACTION49(chain D and resid 41:45)
50X-RAY DIFFRACTION50(chain D and resid 46:50)
51X-RAY DIFFRACTION51(chain G and resid 1)
52X-RAY DIFFRACTION52Chain D and resi 51:53

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