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Open data
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Basic information
Entry | Database: PDB / ID: 3teq | ||||||
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Title | Crystal structure of SOAR domain | ||||||
![]() | Stromal interaction molecule 1 | ||||||
![]() | SIGNALING PROTEIN | ||||||
Function / homology | ![]() store-operated calcium entry / activation of store-operated calcium channel activity / regulation of store-operated calcium entry / enamel mineralization / Elevation of cytosolic Ca2+ levels / cortical endoplasmic reticulum / positive regulation of adenylate cyclase activity / microtubule plus-end binding / plasma membrane raft / regulation of calcium ion transport ...store-operated calcium entry / activation of store-operated calcium channel activity / regulation of store-operated calcium entry / enamel mineralization / Elevation of cytosolic Ca2+ levels / cortical endoplasmic reticulum / positive regulation of adenylate cyclase activity / microtubule plus-end binding / plasma membrane raft / regulation of calcium ion transport / calcium channel regulator activity / detection of calcium ion / Ion homeostasis / sarcoplasmic reticulum membrane / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / intracellular calcium ion homeostasis / positive regulation of angiogenesis / microtubule / protease binding / calcium ion binding / endoplasmic reticulum membrane / endoplasmic reticulum / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Yang, X. / Jin, H. / Cai, X. / Shen, Y. | ||||||
![]() | ![]() Title: Structural and mechanistic insights into the activation of Stromal interaction molecule 1 (STIM1). Authors: Yang, X. / Jin, H. / Cai, X. / Li, S. / Shen, Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 105.1 KB | Display | ![]() |
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PDB format | ![]() | 82.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 472.7 KB | Display | ![]() |
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Full document | ![]() | 482 KB | Display | |
Data in XML | ![]() | 25.4 KB | Display | |
Data in CIF | ![]() | 36.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 11713.393 Da / Num. of mol.: 4 / Fragment: SOAR domain (UNP RESIDUES 344-444) / Mutation: L374M, V419A, C437T Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-PO4 / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 46.45 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: 0.1M Bis-Tris pH 6.5, 10% PEG3350, 0.2M Ammonium dibasic phosphate, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
-Data collection
Diffraction |
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Detector |
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Radiation |
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Radiation wavelength | Wavelength: 0.9792 Å / Relative weight: 1 | ||||||||||||||||||
Reflection | Resolution: 1.8→50 Å / Num. all: 40114 / Num. obs: 36416 / % possible obs: 90.8 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 13.5 % / Biso Wilson estimate: 19.2 Å2 / Rsym value: 0.079 / Net I/σ(I): 2.9 | ||||||||||||||||||
Reflection shell | Resolution: 1.8→1.86 Å / % possible all: 66.3 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 57.9755 Å2 / ksol: 0.4 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 28.5 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.9→25.2 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: CONSTR | ||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 1.9→2.02 Å / Rfactor Rfree error: 0.02 / Total num. of bins used: 6
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Xplor file |
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