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Yorodumi- PDB-3sp8: Crystal structure of NK2 in complex with fractionated Heparin DP10 -
+Open data
-Basic information
Entry | Database: PDB / ID: 3sp8 | ||||||
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Title | Crystal structure of NK2 in complex with fractionated Heparin DP10 | ||||||
Components | Hepatocyte growth factor alpha chain | ||||||
Keywords | HORMONE / kringle domain / MET Tyrosine Kinase | ||||||
Function / homology | Function and homology information regulation of p38MAPK cascade / regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling / Drug-mediated inhibition of MET activation / skeletal muscle cell proliferation / MET activates STAT3 / negative regulation of hydrogen peroxide-mediated programmed cell death / MET interacts with TNS proteins / MET Receptor Activation / hepatocyte growth factor receptor signaling pathway / MET receptor recycling ...regulation of p38MAPK cascade / regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling / Drug-mediated inhibition of MET activation / skeletal muscle cell proliferation / MET activates STAT3 / negative regulation of hydrogen peroxide-mediated programmed cell death / MET interacts with TNS proteins / MET Receptor Activation / hepatocyte growth factor receptor signaling pathway / MET receptor recycling / MET activates PTPN11 / MET activates RAP1 and RAC1 / myoblast proliferation / MET activates PI3K/AKT signaling / MET activates PTK2 signaling / positive regulation of DNA biosynthetic process / cellular response to hepatocyte growth factor stimulus / negative regulation of release of cytochrome c from mitochondria / chemoattractant activity / negative regulation of interleukin-6 production / positive regulation of interleukin-10 production / epithelial to mesenchymal transition / positive regulation of osteoblast differentiation / MET activates RAS signaling / negative regulation of peptidyl-serine phosphorylation / negative regulation of extrinsic apoptotic signaling pathway via death domain receptors / Interleukin-7 signaling / cell chemotaxis / negative regulation of autophagy / platelet alpha granule lumen / liver development / epithelial cell proliferation / growth factor activity / cell morphogenesis / Negative regulation of MET activity / negative regulation of inflammatory response / Constitutive Signaling by Aberrant PI3K in Cancer / positive regulation of peptidyl-tyrosine phosphorylation / Platelet degranulation / PIP3 activates AKT signaling / mitotic cell cycle / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / RAF/MAP kinase cascade / Interleukin-4 and Interleukin-13 signaling / positive regulation of MAPK cascade / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of cell migration / positive regulation of protein phosphorylation / signaling receptor binding / negative regulation of apoptotic process / positive regulation of transcription by RNA polymerase II / extracellular space / extracellular region / membrane / identical protein binding Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.86 Å | ||||||
Authors | Recacha, R. / Mulloy, B. / Gherardi, E. | ||||||
Citation | Journal: TO BE PUBLISHED Title: Crystal structure of NK2 in complex with fractionated Heparin DP10 Authors: Recacha, R. / Mulloy, B. / Gherardi, E. #1: Journal: Embo J. / Year: 2001 Title: Crystal structures of NK1 heparin complexes reveal the basis for NK1 activity and enable engineering of potent agonists of the MET receptor Authors: Lietha, D. / Chirgadze, D.Y. / L Blundell, T. / Gherardi, E. / Mulloy, B. #2: Journal: Proc.Natl.Acad.Sci.USA / Year: 2010 Title: Structural basis for agonism and antagonism of hepatocyte growth factor Authors: Tolbert, W.D. / Daugherty-Holtrop, J. / Vande Woude, G. / Xu, H.E. / Gherardi, E. #3: Journal: J.Biol.Chem. / Year: 2008 Title: Heparin-induced cis- and trans-Dimerization Modes of the Thrombospondin-1 N-terminal Domain Authors: Tan, K. / Duquette, M. / Liu, J.H. / Shanmugasundaram, K. / Joachimiak, A. / Gallagher, J.T. / Rigby, A.C. / Wang, J.H. / Lawler, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3sp8.cif.gz | 244 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3sp8.ent.gz | 196.7 KB | Display | PDB format |
PDBx/mmJSON format | 3sp8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sp/3sp8 ftp://data.pdbj.org/pub/pdb/validation_reports/sp/3sp8 | HTTPS FTP |
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-Related structure data
Related structure data | 3nh4S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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Unit cell |
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-Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 30910.119 Da / Num. of mol.: 2 / Fragment: NK2 / Mutation: G146D, C214S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: HGF, HPTA / Production host: Pichia Pastoris (fungus) / References: UniProt: P14210 |
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-Non-polymers , 5 types, 378 molecules
#2: Chemical | ChemComp-MRD / ( #3: Chemical | ChemComp-MES / #4: Chemical | ChemComp-MPD / ( | #5: Chemical | ChemComp-SO4 / #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.69 Å3/Da / Density % sol: 54.29 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 6 Details: 50mM MES pH=6.0, 30% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 21K, temperature 294K |
-Data collection
Diffraction | Mean temperature: 172 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID29 / Wavelength: 0.97 Å |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Jan 29, 2011 |
Radiation | Monochromator: Si / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97 Å / Relative weight: 1 |
Reflection | Resolution: 1.86→65.8 Å / Num. all: 107330 / Num. obs: 54379 / % possible obs: 99.76 % / Observed criterion σ(F): 1.38 |
Reflection shell | Highest resolution: 1.86 Å / % possible all: 99.76 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3NH4 Resolution: 1.86→65.8 Å / SU ML: 0.57 / Cross valid method: Maximum Likelyhood / σ(F): 1.38 / Phase error: 22.22 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.83 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 45.503 Å2 / ksol: 0.329 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 1.86→65.8 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: -29.1929 Å / Origin y: 26.8444 Å / Origin z: -16.1371 Å
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Refinement TLS group | Selection details: all |