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- PDB-3siu: Structure of a hPrp31-15.5K-U4atac 5' stem loop complex, monomeri... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3siu | ||||||
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Title | Structure of a hPrp31-15.5K-U4atac 5' stem loop complex, monomeric form | ||||||
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![]() | SPLICING/RNA / Major and minor spliceosome / RNA-protein complex / U4 snRNP and U4atac snRNP / RNA-binding protein / pre-mRNA splicing / U4 snRNA / nucleus / SPLICING-RNA complex | ||||||
Function / homology | ![]() ribonucleoprotein complex localization / snRNP binding / U4atac snRNP / U4atac snRNA binding / box C/D sno(s)RNA binding / dense fibrillar component / spliceosomal tri-snRNP complex / U4 snRNA binding / box C/D methylation guide snoRNP complex / U2-type spliceosomal complex ...ribonucleoprotein complex localization / snRNP binding / U4atac snRNP / U4atac snRNA binding / box C/D sno(s)RNA binding / dense fibrillar component / spliceosomal tri-snRNP complex / U4 snRNA binding / box C/D methylation guide snoRNP complex / U2-type spliceosomal complex / U2-type precatalytic spliceosome / U4 snRNP / box C/D snoRNP assembly / rRNA modification in the nucleus and cytosol / U3 snoRNA binding / precatalytic spliceosome / MLL1 complex / spliceosomal tri-snRNP complex assembly / single fertilization / Major pathway of rRNA processing in the nucleolus and cytosol / ribonucleoprotein complex binding / Cajal body / U4/U6 x U5 tri-snRNP complex / mRNA Splicing - Major Pathway / maturation of LSU-rRNA / maturation of SSU-rRNA / small-subunit processome / mRNA splicing, via spliceosome / protein-macromolecule adaptor activity / ribosomal small subunit biogenesis / ATPase binding / cytosolic large ribosomal subunit / nuclear speck / nucleolus / protein-containing complex / RNA binding / nucleoplasm / identical protein binding / nucleus Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Liu, S. / Ghalei, H. / Luhrmann, R. / Wahl, M.C. | ||||||
![]() | ![]() Title: Structural basis for the dual U4 and U4atac snRNA-binding specificity of spliceosomal protein hPrp31. Authors: Liu, S. / Ghalei, H. / Luhrmann, R. / Wahl, M.C. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 185.8 KB | Display | ![]() |
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PDB format | ![]() | 143.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 511.3 KB | Display | ![]() |
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Full document | ![]() | 534.7 KB | Display | |
Data in XML | ![]() | 30.8 KB | Display | |
Data in CIF | ![]() | 42.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3sivC ![]() 2ozbS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 14335.656 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein | Mass: 28268.225 Da / Num. of mol.: 2 / Fragment: UNP residues 85-333 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #3: RNA chain | Mass: 9073.462 Da / Num. of mol.: 2 / Fragment: GB bases 28-55 / Source method: obtained synthetically / Source: (synth.) ![]() #4: Chemical | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.89 Å3/Da / Density % sol: 57.46 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 0.2 M lithium sulfate, 0.1 M Bis-Tris, pH 7.0, 20% w/v PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 393K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Jan 10, 2007 / Details: mirrors |
Radiation | Monochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→30 Å / Num. all: 34765 / Num. obs: 34765 / % possible obs: 100 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 5.7 % / Biso Wilson estimate: 66.9 Å2 / Rmerge(I) obs: 0.055 / Rsym value: 0.055 / Net I/σ(I): 19.5 |
Reflection shell | Resolution: 2.6→2.7 Å / Redundancy: 4.9 % / Rmerge(I) obs: 0.652 / Mean I/σ(I) obs: 1.5 / Num. unique all: 3849 / Rsym value: 0.652 / % possible all: 99.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ID 2OZB Resolution: 2.626→29.45 Å / SU ML: 0.41 / Isotropic thermal model: ISOTROPIC / Cross valid method: THROUGHOUT / σ(F): 1.35 / σ(I): 0 / Phase error: 30.73 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 63.641 Å2 / ksol: 0.329 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 80.4 Å2
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Refinement step | Cycle: LAST / Resolution: 2.626→29.45 Å
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Refine LS restraints |
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LS refinement shell |
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