Knl1/Spc105 complex / positive regulation of meiosis I spindle assembly checkpoint / homologous chromosome orientation in meiotic metaphase I / mitotic checkpoint complex / acrosome assembly / meiotic sister chromatid cohesion, centromeric / regulation of mitotic cell cycle spindle assembly checkpoint / attachment of spindle microtubules to kinetochore / Inactivation of APC/C via direct inhibition of the APC/C complex / APC/C:Cdc20 mediated degradation of mitotic proteins ...Knl1/Spc105 complex / positive regulation of meiosis I spindle assembly checkpoint / homologous chromosome orientation in meiotic metaphase I / mitotic checkpoint complex / acrosome assembly / meiotic sister chromatid cohesion, centromeric / regulation of mitotic cell cycle spindle assembly checkpoint / attachment of spindle microtubules to kinetochore / Inactivation of APC/C via direct inhibition of the APC/C complex / APC/C:Cdc20 mediated degradation of mitotic proteins / anaphase-promoting complex / metaphase/anaphase transition of mitotic cell cycle / outer kinetochore / protein localization to chromosome, centromeric region / protein localization to kinetochore / mitotic spindle assembly checkpoint signaling / mitotic sister chromatid segregation / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Deposition of new CENPA-containing nucleosomes at the centromere / Resolution of Sister Chromatid Cohesion / APC-Cdc20 mediated degradation of Nek2A / acrosomal vesicle / RHO GTPases Activate Formins / Cdc20:Phospho-APC/C mediated degradation of Cyclin A / kinetochore / spindle / Separation of Sister Chromatids / nuclear body / non-specific serine/threonine protein kinase / protein kinase activity / phosphorylation / cell division / protein serine kinase activity / protein serine/threonine kinase activity / centrosome / apoptotic process / perinuclear region of cytoplasm / nucleoplasm / ATP binding / nucleus / cytoplasm / cytosol Similarity search - Function
Mitoticcheckpointserine/threonine-proteinkinaseBUB1beta / MAD3/BUB1-related protein kinase / hBUBR1 / Mitotic checkpoint kinase MAD3L / Protein SSK1
Mass: 20578.621 Da / Num. of mol.: 2 / Fragment: UNP residues 67-220 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: BUB1B, BUBR1, MAD3L, SSK1 / Plasmid: PGEX / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 References: UniProt: O60566, non-specific serine/threonine protein kinase
#2: Protein/peptide
ProteinCASC5 / ALL1-fused gene from chromosome 15q14 protein / AF15q14 / Bub-linking kinetochore protein / Blinkin ...ALL1-fused gene from chromosome 15q14 protein / AF15q14 / Bub-linking kinetochore protein / Blinkin / Cancer susceptibility candidate gene 5 protein / Cancer/testis antigen 29 / CT29 / Kinetochore-null protein 1 / Protein D40/AF15q14
Mass: 2657.010 Da / Num. of mol.: 2 / Fragment: UNP residues 234-252 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CASC5, KIAA1570, KNL1 / Plasmid: PGEX / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: Q8NG31
Resolution: 2.2→38.2 Å / Cor.coef. Fo:Fc: 0.937 / Cor.coef. Fo:Fc free: 0.902 / SU B: 13.612 / SU ML: 0.159 / Cross valid method: THROUGHOUT / ESU R Free: 0.225 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.24665
985
5.1 %
RANDOM
Rwork
0.19343
-
-
-
all
0.19626
18292
-
-
obs
0.19626
18278
99.92 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK
Displacement parameters
Biso mean: 24.773 Å2
Baniso -1
Baniso -2
Baniso -3
1-
-0.02 Å2
-0 Å2
0 Å2
2-
-
0.01 Å2
0 Å2
3-
-
-
0.01 Å2
Refinement step
Cycle: LAST / Resolution: 2.2→38.2 Å
Protein
Nucleic acid
Ligand
Solvent
Total
Num. atoms
2756
0
0
250
3006
Refine LS restraints
Refine-ID
Type
Dev ideal
Dev ideal target
Number
X-RAY DIFFRACTION
r_bond_refined_d
0.011
0.022
2866
X-RAY DIFFRACTION
r_angle_refined_deg
1.116
1.93
3875
X-RAY DIFFRACTION
r_dihedral_angle_1_deg
5.113
5
340
X-RAY DIFFRACTION
r_dihedral_angle_2_deg
40.501
23.908
174
X-RAY DIFFRACTION
r_dihedral_angle_3_deg
15.141
15
491
X-RAY DIFFRACTION
r_dihedral_angle_4_deg
17.699
15
28
X-RAY DIFFRACTION
r_chiral_restr
0.074
0.2
385
X-RAY DIFFRACTION
r_gen_planes_refined
0.005
0.021
2290
X-RAY DIFFRACTION
r_mcbond_it
0.603
1.5
1667
X-RAY DIFFRACTION
r_mcangle_it
1.133
2
2658
X-RAY DIFFRACTION
r_scbond_it
1.655
3
1199
X-RAY DIFFRACTION
r_scangle_it
2.733
4.5
1211
LS refinement shell
Resolution: 2.2→2.257 Å / Total num. of bins used: 20
Rfactor
Num. reflection
% reflection
Rfree
0.28
70
-
Rwork
0.2
1347
-
obs
-
-
100 %
Refinement TLS params.
Method: refined / Refine-ID: X-RAY DIFFRACTION
ID
L11 (°2)
L12 (°2)
L13 (°2)
L22 (°2)
L23 (°2)
L33 (°2)
S11 (Å °)
S12 (Å °)
S13 (Å °)
S21 (Å °)
S22 (Å °)
S23 (Å °)
S31 (Å °)
S32 (Å °)
S33 (Å °)
T11 (Å2)
T12 (Å2)
T13 (Å2)
T22 (Å2)
T23 (Å2)
T33 (Å2)
Origin x (Å)
Origin y (Å)
Origin z (Å)
1
0.3243
0.135
0.4737
0.1335
0.1689
0.6627
0.0106
-0.0138
0.005
0.0193
-0.0257
0.0016
0.0243
-0.0326
0.0152
0.1553
-0.0096
-0.0058
0.1385
0.0003
0.1604
14.7898
-0.1732
31.4405
2
0.3099
0.2216
-0.5514
0.1606
-0.4061
1.4972
0.0035
-0.0092
-0.0182
-0.0026
-0.029
0.0019
-0.0591
-0.0215
0.0255
0.16
-0.0028
-0.0101
0.1301
-0.0043
0.1499
47.6858
9.7792
13.6067
3
10.8371
-5.6074
-0.2818
9.1263
1.7082
0.0347
0.2274
0.3935
-0.1987
-0.6638
-0.1728
0.1254
-0.1653
0.139
-0.0546
0.1951
-0.033
0.003
0.2051
-0.0424
0.1001
19.09
7.7074
17.5814
4
17.5119
0.8577
-1.9466
23.3269
15.4918
10.9089
0.4008
-0.0045
-0.1507
-0.6329
0.0663
0.2502
-0.2076
-1.3103
-0.4671
0.0294
-0.208
-0.1167
0.8969
0.4503
0.1625
34.223
5.7306
6.0214
Refinement TLS group
ID
Refine-ID
Refine TLS-ID
Auth asym-ID
Auth seq-ID
1
X-RAY DIFFRACTION
1
A
54 - 205
2
X-RAY DIFFRACTION
2
B
55 - 203
3
X-RAY DIFFRACTION
3
X
2 - 19
4
X-RAY DIFFRACTION
4
Y
6 - 18
+
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