- PDB-3puw: Crystal Structure of an outward-facing MBP-Maltose transporter co... -
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基本情報
登録情報
データベース: PDB / ID: 3puw
タイトル
Crystal Structure of an outward-facing MBP-Maltose transporter complex bound to ADP-AlF4
要素
(Maltose transport system permease protein ...) x 2
Maltose-binding periplasmic protein
Maltose/maltodextrin import ATP-binding protein MalK
キーワード
HYDROLASE/TRANSPORT PROTEIN / ATP Binding Cassette Nucleotide Binding Domain Substrate Binding Protein Transmembrane Domain / ABC Transporter importer ATPase / ATP binding Maltodextrin binding / transmembrane integral membrane / HYDROLASE-TRANSPORT PROTEIN complex
機能・相同性
機能・相同性情報
ABC-type maltose transporter / ABC-type maltose transporter activity / negative regulation of maltose transport / enzyme IIA-maltose transporter complex / negative regulation of transmembrane transport / detection of maltose stimulus / maltose transport complex / carbohydrate transport / carbohydrate transmembrane transporter activity / maltose binding ...ABC-type maltose transporter / ABC-type maltose transporter activity / negative regulation of maltose transport / enzyme IIA-maltose transporter complex / negative regulation of transmembrane transport / detection of maltose stimulus / maltose transport complex / carbohydrate transport / carbohydrate transmembrane transporter activity / maltose binding / maltose transport / maltodextrin transmembrane transport / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / ATP-binding cassette (ABC) transporter complex / cell chemotaxis / outer membrane-bounded periplasmic space / DNA-binding transcription factor binding / periplasmic space / DNA damage response / ATP hydrolysis activity / ATP binding / membrane / plasma membrane 類似検索 - 分子機能
MalF N-terminal region-like / Periplasmic binding protein-like II / D-maltodextrin-binding protein, MBP / MalF N-terminal region-like / MalF N-terminal region-like / Maltose transport system permease protein MalF, P2 domain / MalF, N-terminal / : / : / Maltose transport system permease protein MalF P2 domain ...MalF N-terminal region-like / Periplasmic binding protein-like II / D-maltodextrin-binding protein, MBP / MalF N-terminal region-like / MalF N-terminal region-like / Maltose transport system permease protein MalF, P2 domain / MalF, N-terminal / : / : / Maltose transport system permease protein MalF P2 domain / MalF, N-terminal region, transmembrane helices / MetI-like fold / MetI-like / Maltose/Maltodextrin import ATP-binding protein malK family profile. / Transport-associated OB, type 2 / TOBE domain / : / MalK OB fold domain / ABC transporter, maltose/maltodextrin import, MalK-like / : / Molybdate/tungstate binding, C-terminal / ABC transporter type 1, transmembrane domain MetI-like / MetI-like superfamily / Binding-protein-dependent transport system inner membrane component / ABC transporter integral membrane type-1 domain profile. / RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain / Maltose/Cyclodextrin ABC transporter, substrate-binding protein / Solute-binding family 1, conserved site / Bacterial extracellular solute-binding proteins, family 1 signature. / Bacterial extracellular solute-binding protein / Bacterial extracellular solute-binding protein / Nucleic acid-binding proteins / Methane Monooxygenase Hydroxylase; Chain G, domain 1 / ABC transporter-like, conserved site / ABC transporters family signature. / Periplasmic binding protein-like II / ABC transporter / ABC transporter-like, ATP-binding domain / ATP-binding cassette, ABC transporter-type domain profile. / D-Maltodextrin-Binding Protein; domain 2 / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / P-loop containing nucleotide triphosphate hydrolases / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / Nucleic acid-binding, OB-fold / Roll / Up-down Bundle / Beta Barrel / Rossmann fold / P-loop containing nucleoside triphosphate hydrolase / Orthogonal Bundle / 3-Layer(aba) Sandwich / Mainly Beta / Mainly Alpha / Alpha Beta 類似検索 - ドメイン・相同性
alpha-maltose / ADENOSINE-5'-DIPHOSPHATE / TETRAFLUOROALUMINATE ION / Chem-PGV / Maltose/maltodextrin transport system permease protein MalF / Maltose/maltodextrin-binding periplasmic protein / Maltose/maltodextrin transport system permease protein MalG / Maltose/maltodextrin import ATP-binding protein MalK 類似検索 - 構成要素
E: Maltose-binding periplasmic protein F: Maltose transport system permease protein malF G: Maltose transport system permease protein malG A: Maltose/maltodextrin import ATP-binding protein MalK B: Maltose/maltodextrin import ATP-binding protein MalK ヘテロ分子
解像度: 2.2→2.28 Å / 冗長度: 2.6 % / Mean I/σ(I) obs: 1.7 / Rsym value: 0.459 / % possible all: 16.6
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解析
ソフトウェア
名称
バージョン
分類
HKL-2000
データ収集
PHASER
位相決定
REFMAC
5.5.0088
精密化
HKL-2000
データ削減
HKL-2000
データスケーリング
精密化
構造決定の手法: 分子置換 / 解像度: 2.3→19.99 Å / Cor.coef. Fo:Fc: 0.931 / Cor.coef. Fo:Fc free: 0.906 / SU B: 17.644 / SU ML: 0.188 / 交差検証法: THROUGHOUT / ESU R: 0.42 / ESU R Free: 0.279 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.2548
4819
5 %
RANDOM
Rwork
0.22022
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obs
0.22194
91058
66.49 %
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all
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93125
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: MASK