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Yorodumi- PDB-3olm: Structure and Function of a Ubiquitin Binding Site within the Cat... -
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-Basic information
Entry | Database: PDB / ID: 3olm | ||||||
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Title | Structure and Function of a Ubiquitin Binding Site within the Catalytic Domain of a HECT Ubiquitin Ligase | ||||||
Components |
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Keywords | LIGASE / ubiquitin E3 ligase | ||||||
Function / homology | Function and homology information regulation of dolichol biosynthetic process / RSP5-BUL ubiquitin ligase complex / regulation of ribosomal large subunit export from nucleus / regulation of tRNA processing / regulation of tRNA export from nucleus / regulation of ubiquinone biosynthetic process / regulation of phosphate metabolic process / regulation of ergosterol biosynthetic process / RHOQ GTPase cycle / Josephin domain DUBs ...regulation of dolichol biosynthetic process / RSP5-BUL ubiquitin ligase complex / regulation of ribosomal large subunit export from nucleus / regulation of tRNA processing / regulation of tRNA export from nucleus / regulation of ubiquinone biosynthetic process / regulation of phosphate metabolic process / regulation of ergosterol biosynthetic process / RHOQ GTPase cycle / Josephin domain DUBs / RHOU GTPase cycle / RAS processing / positive regulation of ubiquitin-dependent endocytosis / Regulation of PTEN localization / ER Quality Control Compartment (ERQC) / UCH proteinases / Pexophagy / regulation of multivesicular body size / Interleukin-1 signaling / Aggrephagy / ribophagy / ubiquitin-dependent endocytosis / Peroxisomal protein import / Synthesis of active ubiquitin: roles of E1 and E2 enzymes / regulation of mRNA export from nucleus / mitochondria-associated ubiquitin-dependent protein catabolic process / Metalloprotease DUBs / regulation of rRNA processing / Endosomal Sorting Complex Required For Transport (ESCRT) / actin cortical patch / cellular bud tip / late endosome to vacuole transport via multivesicular body sorting pathway / E3 ubiquitin ligases ubiquitinate target proteins / nonfunctional rRNA decay / Translesion Synthesis by POLH / positive regulation of fatty acid biosynthetic process / Translesion synthesis by REV1 / Translesion synthesis by POLK / Translesion synthesis by POLI / regulation of nitrogen utilization / ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / HECT-type E3 ubiquitin transferase / Termination of translesion DNA synthesis / Negative regulators of DDX58/IFIH1 signaling / poly(A)+ mRNA export from nucleus / Ubiquitin Mediated Degradation of Phosphorylated Cdc25A / Regulation of PTEN stability and activity / KEAP1-NFE2L2 pathway / CDK-mediated phosphorylation and removal of Cdc6 / Neddylation / FBXL7 down-regulates AURKA during mitotic entry and in early mitosis / Formation of TC-NER Pre-Incision Complex / Orc1 removal from chromatin / MAPK6/MAPK4 signaling / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / positive regulation of endocytosis / Gap-filling DNA repair synthesis and ligation in TC-NER / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / protein K63-linked ubiquitination / Antigen processing: Ubiquitination & Proteasome degradation / L13a-mediated translational silencing of Ceruloplasmin expression / Dual incision in TC-NER / Ub-specific processing proteases / cytosolic ribosome / ubiquitin ligase complex / mitochondrion organization / phosphatidylinositol binding / ubiquitin binding / regulation of actin cytoskeleton organization / positive regulation of receptor-mediated endocytosis / modification-dependent protein catabolic process / ubiquitin-protein transferase activity / protein tag activity / regulation of protein localization / ubiquitin protein ligase activity / positive regulation of proteasomal ubiquitin-dependent protein catabolic process / chromatin organization / ubiquitin-dependent protein catabolic process / proteasome-mediated ubiquitin-dependent protein catabolic process / endosome membrane / protein ubiquitination / ubiquitin protein ligase binding / Golgi apparatus / positive regulation of transcription by RNA polymerase II / mitochondrion / nucleus / plasma membrane / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.495 Å | ||||||
Authors | Kim, H.C. / Steffen, A. / Chen, J. / Huibregtse, J.M. | ||||||
Citation | Journal: Embo Rep. / Year: 2011 Title: Structure and function of a HECT domain ubiquitin-binding site. Authors: Kim, H.C. / Steffen, A.M. / Oldham, M.L. / Chen, J. / Huibregtse, J.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3olm.cif.gz | 211.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3olm.ent.gz | 169 KB | Display | PDB format |
PDBx/mmJSON format | 3olm.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 3olm_validation.pdf.gz | 437.7 KB | Display | wwPDB validaton report |
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Full document | 3olm_full_validation.pdf.gz | 444.2 KB | Display | |
Data in XML | 3olm_validation.xml.gz | 19.3 KB | Display | |
Data in CIF | 3olm_validation.cif.gz | 25.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ol/3olm ftp://data.pdbj.org/pub/pdb/validation_reports/ol/3olm | HTTPS FTP |
-Related structure data
Related structure data | 1nd7S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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-Components
#1: Protein | Mass: 50366.660 Da / Num. of mol.: 1 / Fragment: WW3 and HECT domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: RSP5, MDP1, NPI1, YER125W, SYGP-ORF41 / Production host: Escherichia coli (E. coli) References: UniProt: P39940, Ligases; Forming carbon-nitrogen bonds; Acid-amino-acid ligases (peptide synthases) |
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#2: Protein | Mass: 8841.026 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: UBI4, SCD2, YLL039C / Production host: Escherichia coli (E. coli) / References: UniProt: P0CG63 |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.44 Å3/Da / Density % sol: 49.67 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 9 Details: 13.5% PEG 4000, 0.2M magnesium chloride, 0.1M Tris-HCl pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 23-ID-D / Wavelength: 1.03326 Å |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Oct 28, 2009 |
Radiation | Monochromator: Double crystal cryo-cooled / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.03326 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→50 Å / Num. obs: 14422 / % possible obs: 75.2 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Biso Wilson estimate: 38.76 Å2 |
Reflection shell | Resolution: 2.5→2.59 Å / % possible all: 33.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1ND7 Resolution: 2.495→42.085 Å / SU ML: 0.38 / σ(F): 1.34 / Phase error: 31.22 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 33.734 Å2 / ksol: 0.321 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.495→42.085 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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