+
Open data
-
Basic information
Entry | Database: PDB / ID: 3o27 | ||||||
---|---|---|---|---|---|---|---|
Title | The crystal structure of C68 from the hybrid virus-plasmid pSSVx | ||||||
![]() | Putative uncharacterized protein | ||||||
![]() | DNA BINDING PROTEIN / swapped-hairpin fold / transcription factor | ||||||
Function / homology | Pemi-like Protein 1; Chain: D / Pemi-like Protein 1; Chain: D - #10 / Ribbon / Mainly Beta / Uncharacterized protein![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | D'Ambrosio, K. / De Simone, G. | ||||||
![]() | ![]() Title: C68 from the Sulfolobus islandicus plasmid-virus pSSVx is a novel member of the AbrB-like transcription factor family. Authors: Contursi, P. / D'Ambrosio, K. / Pirone, L. / Pedone, E. / Aucelli, T. / She, Q. / De Simone, G. / Bartolucci, S. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 35.2 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 25.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 430.5 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 432.5 KB | Display | |
Data in XML | ![]() | 7.2 KB | Display | |
Data in CIF | ![]() | 8.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 7757.146 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 3.36 Å3/Da / Density % sol: 63.36 % |
---|---|
Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 3.6 Details: 1.6 M sodium formate, 0.1 M Sodium acetate, pH 3.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Oct 29, 2008 |
Radiation | Monochromator: Graphite / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→50 Å / Num. all: 5275 / Num. obs: 5275 / % possible obs: 100 % / Redundancy: 9.6 % / Rmerge(I) obs: 0.059 / Net I/σ(I): 0.393 |
Reflection shell | Resolution: 2.8→2.9 Å / Redundancy: 9 % / Rmerge(I) obs: 0.444 / Mean I/σ(I) obs: 5.3 / Num. unique all: 528 / % possible all: 99.8 |
-
Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]()
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.8→50 Å
| ||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||
LS refinement shell | Resolution: 2.8→2.93 Å /
|