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- PDB-3hqc: Crystal structure of Phosphotyrosine-binding domain from the Huma... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3hqc | ||||||
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Title | Crystal structure of Phosphotyrosine-binding domain from the Human Tensin-like C1 domain-containing phosphatase (TENC1) | ||||||
![]() | Tensin-like C1 domain-containing phosphatase | ||||||
![]() | HYDROLASE / Human Tensin-like C1 domain-containing phosphatase / TENC1 / Phosphotyrosine Binding Domain / PTB / TNS2 / KIAA1075 / STRUCTURAL GENOMICS / PSI-2 / PROTEIN STRUCTURE INITIATIVE / NEW YORK SGX Research Center for Structural Genomics / NYSGXRC / Cell junction / Cell membrane / Membrane / Metal-binding / Phorbol-ester binding / Phosphoprotein / Protein phosphatase / SH2 domain / Zinc-finger | ||||||
Function / homology | ![]() multicellular organismal-level homeostasis / collagen metabolic process / cellular homeostasis / response to muscle activity / peptidyl-tyrosine dephosphorylation / negative regulation of insulin receptor signaling pathway / protein-tyrosine-phosphatase / protein tyrosine phosphatase activity / kidney development / multicellular organism growth ...multicellular organismal-level homeostasis / collagen metabolic process / cellular homeostasis / response to muscle activity / peptidyl-tyrosine dephosphorylation / negative regulation of insulin receptor signaling pathway / protein-tyrosine-phosphatase / protein tyrosine phosphatase activity / kidney development / multicellular organism growth / kinase binding / negative regulation of cell population proliferation / focal adhesion / lipid binding / identical protein binding / metal ion binding / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Sampathkumar, P. / Romero, R. / Wasserman, S. / Do, J. / Dickey, M. / Bain, K. / Gheyi, T. / Klemke, R. / Atwell, S. / Sauder, J.M. ...Sampathkumar, P. / Romero, R. / Wasserman, S. / Do, J. / Dickey, M. / Bain, K. / Gheyi, T. / Klemke, R. / Atwell, S. / Sauder, J.M. / Burley, S.K. / New York SGX Research Center for Structural Genomics (NYSGXRC) | ||||||
![]() | ![]() Title: Crystal structure of Phosphotyrosine-binding domain from the Human Tensin-like C1 domain-containing phosphatase (TENC1) Authors: Sampathkumar, P. / Romero, R. / Wasserman, S. / Do, J. / Dickey, M. / Bain, K. / Gheyi, T. / Klemke, R. / Atwell, S. / Sauder, J.M. / Burley, S.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 46.8 KB | Display | ![]() |
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PDB format | ![]() | 31.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 463 KB | Display | ![]() |
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Full document | ![]() | 463.9 KB | Display | |
Data in XML | ![]() | 9 KB | Display | |
Data in CIF | ![]() | 11.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1wvhS S: Starting model for refinement |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 17310.248 Da / Num. of mol.: 1 Fragment: Phosphotyrosine Binding Domain, PTB, residues 1264-1409 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: Q63HR2, Hydrolases; Acting on ester bonds; Phosphoric-monoester hydrolases | ||||||
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#2: Chemical | #3: Chemical | #4: Chemical | ChemComp-GOL / | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.58 Å3/Da / Density % sol: 65.64 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, sitting drop / pH: 4 Details: 100mM Sodium Acetate pH 4 + 2% Glycerol + 2000mM Ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Feb 16, 2009 |
Radiation | Monochromator: diamond / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9793 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→47.14 Å / Num. obs: 23089 / % possible obs: 100 % / Redundancy: 10.9 % / Biso Wilson estimate: 25.92 Å2 / Rsym value: 0.118 / Net I/σ(I): 8.3 |
Reflection shell | Resolution: 1.8→1.9 Å / Redundancy: 10.7 % / Mean I/σ(I) obs: 3 / Num. unique all: 3337 / Rsym value: 0.637 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: Poly-Alanine model of the PDB entry 1WVH Resolution: 1.8→26.16 Å / Cor.coef. Fo:Fc: 0.952 / Cor.coef. Fo:Fc free: 0.936 / Occupancy max: 1 / Occupancy min: 0.25 / SU B: 2.5 / SU ML: 0.078 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.105 / ESU R Free: 0.105 Stereochemistry target values: MAXIMUM LIKELIHOOD WITH PHASES Details: There are residual postive electron density features which could not be assigned unambigously as protein or buffer components.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 79.88 Å2 / Biso mean: 33.104 Å2 / Biso min: 17.95 Å2
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Refinement step | Cycle: LAST / Resolution: 1.8→26.16 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.8→1.847 Å / Total num. of bins used: 20
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