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- PDB-3mht: TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE WITH UNMODIFIED DNA A... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3mht | ||||||
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Title | TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE WITH UNMODIFIED DNA AND ADOHCY | ||||||
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![]() | TRANSFERASE/DNA / COMPLEX (METHYLTRANSFERASE-DNA) / TRANSFERASE / METHYLTRANSFERASE / RESTRICTION SYSTEM / TRANSFERASE-DNA complex | ||||||
Function / homology | ![]() DNA (cytosine-5-)-methyltransferase / DNA (cytosine-5-)-methyltransferase activity / DNA restriction-modification system / methylation / DNA binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | O'Gara, M. / Klimasauskas, S. / Roberts, R.J. / Cheng, X. | ||||||
![]() | ![]() Title: Enzymatic C5-cytosine methylation of DNA: mechanistic implications of new crystal structures for HhaL methyltransferase-DNA-AdoHcy complexes. Authors: O'Gara, M. / Klimasauskas, S. / Roberts, R.J. / Cheng, X. #1: ![]() Title: HhaI Methyltransferase Flips its Target Base Out of the DNA Helix Authors: Klimasauskas, S. / Kumar, S. / Roberts, R.J. / Cheng, X. #2: ![]() Title: Crystal Structure of the HhaI DNA Methyltransferase Complexed with S-Adenosyl- L-Methionine Authors: Cheng, X. / Kumar, S. / Posfai, J. / Pflugrath, J.W. / Roberts, R.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 97.6 KB | Display | ![]() |
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PDB format | ![]() | 71 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 465.8 KB | Display | ![]() |
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Full document | ![]() | 497.7 KB | Display | |
Data in XML | ![]() | 12.9 KB | Display | |
Data in CIF | ![]() | 18.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: DNA chain | Mass: 3662.404 Da / Num. of mol.: 1 / Source method: obtained synthetically |
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#2: DNA chain | Mass: 3966.597 Da / Num. of mol.: 1 / Source method: obtained synthetically |
#3: Protein | Mass: 37042.207 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
#4: Chemical | ChemComp-SAH / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.47 Å3/Da / Density % sol: 64.55 % | |||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 16 ℃ / pH: 5.6 / Method: unknown | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: ![]() ![]() ![]() |
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Detector | Type: MARRESEARCH / Detector: IMAGE PLATE |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | % possible obs: 88.7 % / Redundancy: 4.2 % / Rmerge(I) obs: 0.071 |
Reflection | *PLUS % possible obs: 88.7 % / Redundancy: 4.2 % |
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Processing
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Refinement | Resolution: 2.7→20 Å / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 2.7→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.7→2.82 Å
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.7 Å / Lowest resolution: 20 Å / σ(F): 2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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