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- PDB-3lpe: Crystal structure of Spt4/5NGN heterodimer complex from Methanoco... -

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Basic information

Entry
Database: PDB / ID: 3lpe
TitleCrystal structure of Spt4/5NGN heterodimer complex from Methanococcus jannaschii
Components
  • DNA-directed RNA polymerase subunit E''
  • Putative transcription antitermination protein nusG
KeywordsTRANSFERASE / Transcription Regulation / Spt4 / Spt5 / NusG / Archaea / evolution / DNA-directed RNA polymerase / Metal-binding / Nucleotidyltransferase / Transcription / Zinc-finger
Function / homology
Function and homology information


transcription elongation-coupled chromatin remodeling / translation elongation factor activity / regulation of DNA-templated transcription elongation / structural constituent of ribosome / ribosome / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / zinc ion binding
Similarity search - Function
Rubrerythrin, domain 2 - #90 / Archaeal transcription elongation factor Spt4 / Transcription elongation factor Spt5, archaeal / Archaeal transcription elongation factor Spt4 superfamily / NusG, N-terminal domain / Spt4/RpoE2 zinc finger / Spt4/RpoE2 zinc finger / Spt4/RpoE2 zinc finger / NGN domain / Transcription elongation factor SPT5 ...Rubrerythrin, domain 2 - #90 / Archaeal transcription elongation factor Spt4 / Transcription elongation factor Spt5, archaeal / Archaeal transcription elongation factor Spt4 superfamily / NusG, N-terminal domain / Spt4/RpoE2 zinc finger / Spt4/RpoE2 zinc finger / Spt4/RpoE2 zinc finger / NGN domain / Transcription elongation factor SPT5 / Early transcription elongation factor of RNA pol II, NGN section / NusG, N-terminal / In Spt5p, this domain may confer affinity for Spt4p. It possesses a RNP-like fold. / NusG, N-terminal domain superfamily / Rubrerythrin, domain 2 / RNA polymerase subunit RPABC4/transcription elongation factor Spt4 / Single Sheet / Ribosomal protein L24/L26, conserved site / KOW (Kyprides, Ouzounis, Woese) motif. / Translation protein SH3-like domain superfamily / KOW motif / KOW / Ribosomal protein L2, domain 2 / Alpha-Beta Plaits / 2-Layer Sandwich / Mainly Beta / Alpha Beta
Similarity search - Domain/homology
Transcription elongation factor Spt5 / Transcription elongation factor Spt4
Similarity search - Component
Biological speciesMethanocaldococcus jannaschii (archaea)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.9 Å
AuthorsHirtreiter, A. / Damsma, G.E. / Cheung, A.C.M. / Klose, D. / Grohmann, D. / Vojnic, E. / Martin, A.C.R. / Cramer, P. / Werner, F.
CitationJournal: Nucleic Acids Res. / Year: 2010
Title: Spt4/5 stimulates transcription elongation through the RNA polymerase clamp coiled-coil motif.
Authors: Hirtreiter, A. / Damsma, G.E. / Cheung, A.C. / Klose, D. / Grohmann, D. / Vojnic, E. / Martin, A.C. / Cramer, P. / Werner, F.
History
DepositionFeb 5, 2010Deposition site: RCSB / Processing site: PDBJ
Revision 1.0Mar 9, 2010Provider: repository / Type: Initial release
Revision 1.1Jul 13, 2011Group: Version format compliance
Revision 1.2Nov 1, 2017Group: Refinement description / Category: software
Revision 1.3Nov 1, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn / struct_ref_seq_dif / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Putative transcription antitermination protein nusG
B: DNA-directed RNA polymerase subunit E''
C: Putative transcription antitermination protein nusG
D: DNA-directed RNA polymerase subunit E''
E: Putative transcription antitermination protein nusG
F: DNA-directed RNA polymerase subunit E''
G: Putative transcription antitermination protein nusG
H: DNA-directed RNA polymerase subunit E''
hetero molecules


Theoretical massNumber of molelcules
Total (without water)66,35512
Polymers66,0938
Non-polymers2624
Water6,287349
1
A: Putative transcription antitermination protein nusG
B: DNA-directed RNA polymerase subunit E''
hetero molecules


Theoretical massNumber of molelcules
Total (without water)16,5893
Polymers16,5232
Non-polymers651
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1880 Å2
ΔGint-14 kcal/mol
Surface area7700 Å2
MethodPISA
2
C: Putative transcription antitermination protein nusG
D: DNA-directed RNA polymerase subunit E''
hetero molecules


Theoretical massNumber of molelcules
Total (without water)16,5893
Polymers16,5232
Non-polymers651
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1880 Å2
ΔGint-14 kcal/mol
Surface area7700 Å2
MethodPISA
3
E: Putative transcription antitermination protein nusG
F: DNA-directed RNA polymerase subunit E''
hetero molecules


Theoretical massNumber of molelcules
Total (without water)16,5893
Polymers16,5232
Non-polymers651
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1870 Å2
ΔGint-14 kcal/mol
Surface area7700 Å2
MethodPISA
4
G: Putative transcription antitermination protein nusG
H: DNA-directed RNA polymerase subunit E''
hetero molecules


Theoretical massNumber of molelcules
Total (without water)16,5893
Polymers16,5232
Non-polymers651
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1880 Å2
ΔGint-14 kcal/mol
Surface area7700 Å2
MethodPISA
Unit cell
Length a, b, c (Å)64.360, 63.970, 83.040
Angle α, β, γ (deg.)90.00, 106.98, 90.00
Int Tables number4
Space group name H-MP1211
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-ID
11
21
31
41
12
22
32
42

NCS domain segments:
Dom-IDComponent-IDEns-IDSelection details
111chain A and (resseq -1:82 )
211chain C and (resseq -1:82 )
311chain E and (resseq -1:82 )
411chain G and (resseq -1:82 )
112chain B and (resseq 1:59 )
212chain D and (resseq 1:59 )
312chain F and (resseq 1:59 )
412chain H and (resseq 1:59 )

NCS ensembles :
ID
1
2

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Components

#1: Protein
Putative transcription antitermination protein nusG / Transcription elongation factor SPT5 NGN


Mass: 9935.523 Da / Num. of mol.: 4 / Fragment: NGN Domain
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Methanocaldococcus jannaschii (archaea)
Gene: MJ0372 / Plasmid: pGEX-2TK / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (Rosetta 2) Novagen / References: UniProt: Q57818
#2: Protein
DNA-directed RNA polymerase subunit E'' / Transcription elongation factor SPT4


Mass: 6587.811 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Methanocaldococcus jannaschii (archaea)
Gene: rpoE2, MJ0396 / Plasmid: pGEX-2TK / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (Rosetta 2) Novagen / References: UniProt: Q57839, DNA-directed RNA polymerase
#3: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Zn
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 349 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.47 Å3/Da / Density % sol: 50.28 %
Crystal growTemperature: 295 K / Method: vapor diffusion, hanging drop / pH: 7
Details: 20-30% PEG 400, 50mM MES pH 5.5-7.0, 30mg/mL, VAPOR DIFFUSION, HANGING DROP, temperature 295K

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Data collection

DiffractionMean temperature: 88 K
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X06SA / Wavelength: 1 Å
DetectorType: PSI PILATUS 6M / Detector: PIXEL / Date: Jun 21, 2009
RadiationMonochromator: Fixed-exit LN2 cooled Double Crystal Monochromator Si(111)
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 1.9→44.4 Å / Num. all: 47050 / Num. obs: 47050 / % possible obs: 92.1 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 1.9 % / Rmerge(I) obs: 0.046
Reflection shellResolution: 1.9→2 Å / Redundancy: 1.9 % / Rmerge(I) obs: 0.6 / Mean I/σ(I) obs: 1.4 / % possible all: 86.4

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Processing

Software
NameVersionClassification
PHASERphasing
PHENIX(phenix.refine)refinement
XDSdata reduction
XSCALEdata scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1RYQ, 3EWG

1ryq

3ewg


Resolution: 1.9→44.355 Å / SU ML: 0.14 / Isotropic thermal model: Isotropic / σ(F): 1.99 / Stereochemistry target values: ML
RfactorNum. reflection% reflectionSelection details
Rfree0.2549 2353 5 %RANDOM
Rwork0.2076 ---
all0.21 47050 --
obs0.21 47043 92.19 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 33.114 Å2 / ksol: 0.315 e/Å3
Displacement parametersBiso mean: 41.136 Å2
Baniso -1Baniso -2Baniso -3
1--0.0308 Å20 Å20.1528 Å2
2---6.1929 Å20 Å2
3---6.2237 Å2
Refinement stepCycle: LAST / Resolution: 1.9→44.355 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4392 0 4 349 4745
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0084464
X-RAY DIFFRACTIONf_angle_d1.0395996
X-RAY DIFFRACTIONf_dihedral_angle_d14.2171712
X-RAY DIFFRACTIONf_chiral_restr0.076712
X-RAY DIFFRACTIONf_plane_restr0.004756
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDTypeRms dev position (Å)
11A640X-RAY DIFFRACTIONPOSITIONAL
12C640X-RAY DIFFRACTIONPOSITIONAL0.036
13E640X-RAY DIFFRACTIONPOSITIONAL0.039
14G640X-RAY DIFFRACTIONPOSITIONAL0.042
21B458X-RAY DIFFRACTIONPOSITIONAL
22D458X-RAY DIFFRACTIONPOSITIONAL0.039
23F458X-RAY DIFFRACTIONPOSITIONAL0.039
24H458X-RAY DIFFRACTIONPOSITIONAL0.044
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 17

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
1.9-1.93880.34341150.307218977
1.9388-1.98090.35491370.2948259992
1.9809-2.0270.34061410.284267493
2.027-2.07770.32381390.2576263693
2.0777-2.13390.29941400.242266094
2.1339-2.19670.26351380.2256262393
2.1967-2.26760.29511400.217266394
2.2676-2.34860.26761420.2211269195
2.3486-2.44260.25961410.2095269595
2.4426-2.55380.25691420.213268594
2.5538-2.68840.29681390.2338264493
2.6884-2.85680.29231380.2279262093
2.8568-3.07740.24141400.2238267493
3.0774-3.3870.25851390.2031262292
3.387-3.87680.21991370.1731261492
3.8768-4.88340.20461400.149266292
4.8834-44.36710.19291450.1622273993
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.148-0.7595-0.70262.4447-2.81151.83690.13280.2779-0.399-0.2418-0.25950.47150.8916-0.5464-0.00260.3130.02120.0020.289-0.03380.2922-6.682-16.18631.6236
21.03210.20650.87530.6454-0.2230.1016-0.0596-0.26520.34140.5649-0.1347-0.3409-0.33130.25160.00380.2916-0.0107-0.05250.234-0.03710.2312-1.4624-5.425239.9858
30.60290.745-0.15240.4140.28690.9154-0.12210.2352-0.2654-0.69870.01160.05910.10490.11770.00020.47180.0178-0.01070.2751-0.02310.2917-4.3018-9.784525.6577
40.15741.22911.17722.2583-0.25291.6012-0.1078-0.207-0.2076-0.4732-0.0049-0.26430.35640.1713-0.00070.25270.04410.0920.19670.06440.22982.1429-5.155433.0036
50.3001-0.230.88750.48730.8102-0.74730.2948-1.26590.26821.6732-0.9106-1.0288-0.58831.0820.00580.4916-0.0554-0.00720.45690.05460.32164.7104-11.38946.4353
60.33840.17780.07081.0020.34081.0695-0.0704-0.17090.1459-0.12690.1393-0.17850.1275-0.02460.00070.2717-0.025-0.01460.2175-0.00250.1976-11.672-18.785451.872
71.3590.23840.90361.1712-0.15372.73990.1258-0.11190.27090.1136-0.04740.2392-0.1329-0.1819-0.00090.2634-0.02530.02870.1942-0.01280.2653-14.4003-18.501352.0535
80.55850.1813-0.0630.7748-0.42470.8727-0.1138-0.1431-0.499-0.1631-0.0535-0.49530.47470.2774-0.00150.34230.00430.05090.22560.02990.3219-1.0537-21.299239.4229
92.75860.0494-0.87771.5125-1.13750.9802-0.0389-0.02220.21770.3113-0.0442-0.40490.32360.24850.00160.2967-0.03040.00010.20610.02260.2979-7.9989-17.946248.7253
102.1160.6732.33492.3828-0.71291.4539-0.45530.1772-0.68420.04850.0976-0.22110.61880.7353-0.00050.23760.0410.08520.3655-0.04440.38558.0162-22.640570.7881
111.6635-1.45461.58882.41721.22320.5709-0.3265-0.58220.86090.11470.15090.4176-0.02990.0830.00880.23560.04050.04710.3162-0.05450.3023-0.4606-16.241777.9003
120.11960.3676-0.0389-0.08590.03180.00810.27520.77510.2543-1.0901-0.25371.0916-0.7908-0.57620.0020.28050.04120.05230.4122-0.01640.35223.2125-15.864364.4508
133.6039-1.52570.85750.7006-0.62392.1128-0.06290.16090.138-0.2256-0.0141-0.02990.05580.2913-0.00040.21550.00110.02340.26240.00090.2022-2.1629-17.327670.1926
140.9892-0.90990.09580.36990.2204-0.2734-0.8603-1.86220.49431.20940.78662.0667-0.8596-0.67730.04850.64970.1521-0.01490.6742-0.21150.44673.6152-10.594786.1698
151.53720.41850.18540.3847-0.35291.1164-0.6661-1.128-0.55980.41940.2644-0.12590.481-0.02640.00340.51510.33280.11210.6660.0680.332711.1098-29.200191.2372
161.64551.25610.28391.19321.45170.0872-0.5955-1.1458-0.38610.74910.38490.14190.1010.17990.00270.41620.36640.17510.77820.17770.262110.56-27.059690.625
170.5067-0.4172-0.61590.2689-0.75560.5177-0.53230.0240.61170.4820.0055-0.7584-0.38840.66440.00040.28350.0611-0.07930.4947-0.01590.505614.4492-15.136478.9005
182.56530.36541.40752.8324-0.04150.8838-1.1447-1.6162-0.13010.67930.743-0.07230.26860.65880.0110.39790.30040.02660.56510.04250.434310.2294-23.145288.5304
191.15360.4364-0.73730.84750.78280.6459-0.07920.19260.5665-0.2620.07160.6884-0.9687-0.8749-0.00220.31970.0624-0.08150.2872-0.00060.3552-26.30410.02868.804
200.4705-0.1768-0.032-0.1185-0.60330.0677-0.7137-0.35570.46830.47040.0899-0.4683-0.14820.43010.00690.26890.0532-0.10210.3154-0.04620.3398-12.4185-5.138581.874
211.131-0.4469-0.30461.09440.64570.1783-0.06720.5339-0.0233-0.7726-0.3291-0.07250.01390.24870.00010.34260.0172-0.05630.294-0.00940.2518-19.6667-5.138766.7403
221.3971-1.07420.56311.22051.61771.1368-0.00010.28310.0358-0.2679-0.0982-0.43640.01950.205300.2507-0.04090.01740.26090.00330.2802-12.593-6.533271.2839
230.8120.21750.22430.1382-0.77170.2301-0.6107-0.8439-0.58750.84050.2489-0.60381.3209-0.0042-0.00220.39660.03820.04860.34760.02610.302-20.0054-13.070485.5152
241.15-0.9391-0.1090.7018-1.17841.68830.00780.11580.15840.17240.06650.0783-0.04070.08570.00010.28720.0211-0.00870.2306-0.01080.2468-29.07316.516491.0379
250.2150.2271-0.56951.0653-0.05851.92440.1811-0.18080.48050.48190.0043-0.1642-0.11430.2045-0.00010.26540.0725-0.06030.274-0.00670.2749-25.60426.29594.473
261.93590.3981-1.48511.55140.16652.1503-0.1802-0.0313-0.0614-0.0419-0.19650.44070.3243-0.3493-0.00060.25970.02590.01160.2549-0.07990.3543-29.174-4.749980.272
270.9359-0.2952-0.06131.7320.09120.044-0.2025-0.1497-0.150.13870.172-0.5754-0.21170.47610.00580.23520.0256-0.01720.2533-0.03360.2675-23.43820.601891.6646
280.0935-0.1651-0.35090.4435-0.30380.2987-0.28-1.05662.0847-0.3524-0.0181.7951-1.6211-1.5428-0.01470.45640.20110.01990.8229-0.36930.9182-42.2701-13.144252.9282
291.4133-0.5056-0.39340.5564-1.44051.1780.13110.3495-0.2957-0.8247-0.58620.06420.1299-0.2810.00240.27970.08390.02240.2652-0.11730.3733-34.2823-22.471841.0113
300.9785-1.09190.06590.7146-0.01661.1204-0.3958-1.14330.66970.7175-0.04740.89220.2434-0.31210.00060.36510.06230.02390.409-0.14920.435-36.0699-21.354154.3117
310.6083-1.4651-1.0231.85750.85180.939-0.25570.3859-0.15860.070.06320.2072-0.0531-0.39740.00060.2392-0.00530.03170.3163-0.0520.3512-32.1333-27.380347.5961
32-0.29750.3997-1.11850.59110.3081-0.01320.39411.4493-0.9586-0.8212-0.82050.2940.78070.23180.00730.55890.18330.14960.60320.00860.4017-26.7714-19.942533.5593
330.0713-0.096-0.21090.9333-0.38030.61730.2351.12240.423-0.6762-0.39840.28750.4392-0.217-00.47980.2588-0.04810.6199-0.04580.4397-46.3777-12.878328.0426
340.58670.8077-0.41110.25160.14621.02721.01641.2980.0093-2.1165-0.77380.13540.17130.42420.00010.76110.4195-0.10320.7287-0.12310.4766-45.5885-12.711426.1553
351.3452-0.0290.26150.8562-0.39360.84270.17520.31691.2504-0.355-0.4637-0.3848-0.5891-0.0806-00.40660.1098-0.00390.37380.03010.5998-34.1119-10.174539.3083
361.15241.3385-0.18271.6261-1.12730.32781.07291.23960.2599-1.5449-1.404-0.4651-0.2232-1.09760.01380.58390.44290.04920.7153-0.00430.6297-40.8049-15.577229.5628
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1(chain A and resid 24:36)
2X-RAY DIFFRACTION2(chain A and resid 37:46)
3X-RAY DIFFRACTION3(chain A and resid 47:61)
4X-RAY DIFFRACTION4(chain A and resid 62:74)
5X-RAY DIFFRACTION5(chain A and resid 75:82)
6X-RAY DIFFRACTION6(chain B and resid 1:9)
7X-RAY DIFFRACTION7(chain B and resid 10:30)
8X-RAY DIFFRACTION8(chain B and resid 31:47)
9X-RAY DIFFRACTION9(chain B and resid 48:59)
10X-RAY DIFFRACTION10(chain C and resid 22:36)
11X-RAY DIFFRACTION11(chain C and resid 37:48)
12X-RAY DIFFRACTION12(chain C and resid 49:54)
13X-RAY DIFFRACTION13(chain C and resid 55:74)
14X-RAY DIFFRACTION14(chain C and resid 75:82)
15X-RAY DIFFRACTION15(chain D and resid 1:14)
16X-RAY DIFFRACTION16(chain D and resid 15:33)
17X-RAY DIFFRACTION17(chain D and resid 34:47)
18X-RAY DIFFRACTION18(chain D and resid 48:59)
19X-RAY DIFFRACTION19(chain E and resid 23:33)
20X-RAY DIFFRACTION20(chain E and resid 34:43)
21X-RAY DIFFRACTION21(chain E and resid 44:59)
22X-RAY DIFFRACTION22(chain E and resid 60:73)
23X-RAY DIFFRACTION23(chain E and resid 74:82)
24X-RAY DIFFRACTION24(chain F and resid 1:19)
25X-RAY DIFFRACTION25(chain F and resid 20:27)
26X-RAY DIFFRACTION26(chain F and resid 28:51)
27X-RAY DIFFRACTION27(chain F and resid 52:59)
28X-RAY DIFFRACTION28(chain G and resid 23:29)
29X-RAY DIFFRACTION29(chain G and resid 30:47)
30X-RAY DIFFRACTION30(chain G and resid 48:60)
31X-RAY DIFFRACTION31(chain G and resid 61:73)
32X-RAY DIFFRACTION32(chain G and resid 74:82)
33X-RAY DIFFRACTION33(chain H and resid 1:14)
34X-RAY DIFFRACTION34(chain H and resid 15:29)
35X-RAY DIFFRACTION35(chain H and resid 30:49)
36X-RAY DIFFRACTION36(chain H and resid 50:59)

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