regulation of cytokinesis, actomyosin contractile ring assembly / centralspindlin complex / positive regulation of mitotic cytokinetic process / regulation of attachment of spindle microtubules to kinetochore / bicellular tight junction assembly / regulation of protein kinase activity / activation of GTPase activity / regulation of small GTPase mediated signal transduction / RHOB GTPase cycle / NRAGE signals death through JNK ...regulation of cytokinesis, actomyosin contractile ring assembly / centralspindlin complex / positive regulation of mitotic cytokinetic process / regulation of attachment of spindle microtubules to kinetochore / bicellular tight junction assembly / regulation of protein kinase activity / activation of GTPase activity / regulation of small GTPase mediated signal transduction / RHOB GTPase cycle / NRAGE signals death through JNK / activation of protein kinase activity / positive regulation of cytokinesis / cleavage furrow / CDC42 GTPase cycle / mitotic cytokinesis / RHOA GTPase cycle / bicellular tight junction / RAC1 GTPase cycle / cellular response to calcium ion / positive regulation of neuron differentiation / GTPase activator activity / positive regulation of GTPase activity / guanyl-nucleotide exchange factor activity / cellular response to ionizing radiation / cell morphogenesis / protein homooligomerization / mitotic spindle / small GTPase binding / cellular response to hydrogen peroxide / positive regulation of protein import into nucleus / G alpha (12/13) signalling events / cell-cell junction / protein transport / nervous system development / positive regulation of canonical NF-kappaB signal transduction / cell cortex / midbody / cell differentiation / nuclear body / intracellular signal transduction / positive regulation of apoptotic process / centrosome / protein homodimerization activity / nucleoplasm / nucleus / cytoplasm / cytosol 類似検索 - 分子機能
解像度: 1.482→25 Å / Cor.coef. Fo:Fc: 0.959 / Cor.coef. Fo:Fc free: 0.95 / WRfactor Rfree: 0.192 / WRfactor Rwork: 0.173 / SU B: 1.098 / SU ML: 0.043 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.07 / ESU R Free: 0.071 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY. ARP/WARP, COOT and the MOLPROBITY server were also used for refinement.
Rfactor
反射数
%反射
Selection details
Rfree
0.205
1570
4.879 %
thin shells (sftools)
Rwork
0.181
-
-
-
obs
0.182
32180
99.929 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: MASK BULK SOLVENT
原子変位パラメータ
Biso mean: 10.175 Å2
Baniso -1
Baniso -2
Baniso -3
1-
-0.296 Å2
0 Å2
0 Å2
2-
-
-0.163 Å2
0 Å2
3-
-
-
0.459 Å2
精密化ステップ
サイクル: LAST / 解像度: 1.482→25 Å
タンパク質
核酸
リガンド
溶媒
全体
原子数
1463
0
3
119
1585
拘束条件
Refine-ID
タイプ
Dev ideal
Dev ideal target
数
X-RAY DIFFRACTION
r_bond_refined_d
0.016
0.022
1538
X-RAY DIFFRACTION
r_bond_other_d
0.001
0.02
1032
X-RAY DIFFRACTION
r_angle_refined_deg
1.551
1.967
2096
X-RAY DIFFRACTION
r_angle_other_deg
0.885
3
2537
X-RAY DIFFRACTION
r_dihedral_angle_1_deg
6.028
5
197
X-RAY DIFFRACTION
r_dihedral_angle_2_deg
30.905
25.616
73
X-RAY DIFFRACTION
r_dihedral_angle_3_deg
12.018
15
261
X-RAY DIFFRACTION
r_dihedral_angle_4_deg
13.13
15
4
X-RAY DIFFRACTION
r_chiral_restr
0.087
0.2
224
X-RAY DIFFRACTION
r_gen_planes_refined
0.007
0.021
1727
X-RAY DIFFRACTION
r_gen_planes_other
0.001
0.02
301
X-RAY DIFFRACTION
r_mcbond_it
0.941
1.5
941
X-RAY DIFFRACTION
r_mcbond_other
0.26
1.5
378
X-RAY DIFFRACTION
r_mcangle_it
1.665
2
1526
X-RAY DIFFRACTION
r_scbond_it
2.604
3
597
X-RAY DIFFRACTION
r_scangle_it
4.148
4.5
563
LS精密化 シェル
Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 20