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- PDB-2cou: Solution structure of the second BRCT domain of epithelial cell t... -

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Basic information

Entry
Database: PDB / ID: 2cou
TitleSolution structure of the second BRCT domain of epithelial cell transforming 2
ComponentsECT2 protein
KeywordsCELL CYCLE / BRCT domain / ECT2 / Rho GTPase / Structural Genomics / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI
Function / homology
Function and homology information


regulation of cytokinesis, actomyosin contractile ring assembly / guanyl-nucleotide exchange factor activity => GO:0005085 / guanyl-nucleotide exchange factor activity => GO:0005085 / : / centralspindlin complex / small GTPase binding => GO:0031267 / NRAGE signals death through JNK / G alpha (12/13) signalling events / regulation of attachment of spindle microtubules to kinetochore / bicellular tight junction assembly ...regulation of cytokinesis, actomyosin contractile ring assembly / guanyl-nucleotide exchange factor activity => GO:0005085 / guanyl-nucleotide exchange factor activity => GO:0005085 / : / centralspindlin complex / small GTPase binding => GO:0031267 / NRAGE signals death through JNK / G alpha (12/13) signalling events / regulation of attachment of spindle microtubules to kinetochore / bicellular tight junction assembly / activation of GTPase activity / regulation of protein kinase activity / positive regulation of cytokinesis / activation of protein kinase activity / cleavage furrow / mitotic cytokinesis / bicellular tight junction / cellular response to calcium ion / positive regulation of neuron differentiation / GTPase activator activity / cellular response to ionizing radiation / protein homooligomerization / cell morphogenesis / mitotic spindle / cellular response to hydrogen peroxide / positive regulation of GTPase activity / positive regulation of protein import into nucleus / cell-cell junction / protein transport / nervous system development / cell cortex / midbody / nuclear body / intracellular signal transduction / positive regulation of apoptotic process / protein homodimerization activity / nucleoplasm / nucleus / cytosol / cytoplasm
Similarity search - Function
Guanine nucleotide exchange factor Ect2 / twin BRCT domain / BRCT domain / Guanine-nucleotide dissociation stimulator, CDC24, conserved site / Dbl homology (DH) domain signature. / Dbl homology (DH) domain superfamily / RhoGEF domain / Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases / Dbl homology (DH) domain / Dbl homology (DH) domain profile. ...Guanine nucleotide exchange factor Ect2 / twin BRCT domain / BRCT domain / Guanine-nucleotide dissociation stimulator, CDC24, conserved site / Dbl homology (DH) domain signature. / Dbl homology (DH) domain superfamily / RhoGEF domain / Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases / Dbl homology (DH) domain / Dbl homology (DH) domain profile. / BRCA1 C Terminus (BRCT) domain / breast cancer carboxy-terminal domain / BRCT domain profile. / BRCT domain / BRCT domain superfamily / PH-like domain superfamily / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
Biological speciesMus musculus (house mouse)
MethodSOLUTION NMR / torsion angle dynamics
AuthorsNagashima, T. / Hayashi, F. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI)
CitationJournal: To be published
Title: Solution structure of the second BRCT domain of epithelial cell transforming 2
Authors: Nagashima, T. / Hayashi, F. / Yokoyama, S.
History
DepositionMay 18, 2005Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Nov 18, 2005Provider: repository / Type: Initial release
Revision 1.1Apr 30, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Mar 9, 2022Group: Data collection / Database references / Derived calculations
Category: database_2 / pdbx_nmr_software ...database_2 / pdbx_nmr_software / pdbx_struct_assembly / pdbx_struct_oper_list / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_nmr_software.name / _struct_ref_seq_dif.details

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: ECT2 protein


Theoretical massNumber of molelcules
Total (without water)12,1471
Polymers12,1471
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / 100target function, structures with the lowest energy
RepresentativeModel #1lowest energy

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Components

#1: Protein ECT2 protein / Epithelial cell transforming sequence 2 oncogene


Mass: 12146.516 Da / Num. of mol.: 1 / Fragment: BRCA1 C-turminus (BRCT) domain
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Description: Cell-free protein synthesis / Plasmid: P041018-12 / References: UniProt: Q07139

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1113D 15N-separated NOESY
1213D 13C-separated NOESY

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Sample preparation

DetailsContents: 1.02mM BRCT domain U-13C,15N; 20mM TrisHCl, 100mM NaCl, 1mM DTT, 0.02% NaN3
Solvent system: 90% H2O/10% D2O
Sample conditionsIonic strength: 120mM / pH: 7.0 / Pressure: ambient / Temperature: 298 K

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NMR measurement

NMR spectrometerType: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 800 MHz

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Processing

NMR software
NameVersionDeveloperClassification
VNMR6.1CVariancollection
NMRPipe20020425Delaglio, F.processing
NMRView5.0.4Johnson, B.A.data analysis
KUJIRA0.901Kobayashi, N.data analysis
CYANA2.0.17Guntert, P.structure solution
CYANA2.0.17Guntert, P.refinement
RefinementMethod: torsion angle dynamics / Software ordinal: 1
NMR representativeSelection criteria: lowest energy
NMR ensembleConformer selection criteria: target function, structures with the lowest energy
Conformers calculated total number: 100 / Conformers submitted total number: 20

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