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- PDB-1ak7: DESTRIN, NMR, 20 STRUCTURES -

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ID or keywords:

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Basic information

Entry
Database: PDB / ID: 1ak7
TitleDESTRIN, NMR, 20 STRUCTURES
ComponentsDESTRIN
KeywordsACTIN DEPOLYMERIZATION FACTOR
Function / homology
Function and homology information


actin filament fragmentation / positive regulation of actin filament depolymerization / actin filament severing / actin filament depolymerization / actin polymerization or depolymerization / cortical actin cytoskeleton / cell motility / actin filament binding / actin cytoskeleton / extracellular exosome / cytoplasm
Similarity search - Function
Destrin / ADF/Cofilin / Actin-depolymerising factor homology domain / Cofilin/tropomyosin-type actin-binding protein / ADF-H domain profile. / Actin depolymerisation factor/cofilin -like domains / Severin / Severin / ADF-H/Gelsolin-like domain superfamily / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
Biological speciesSus scrofa (pig)
MethodSOLUTION NMR / DYNAMICAL SIMULATED ANNEALING
AuthorsHatanaka, H. / Moriyama, K. / Ogura, K. / Ichikawa, S. / Yahara, I. / Inagaki, F.
CitationJournal: Cell(Cambridge,Mass.) / Year: 1996
Title: Tertiary structure of destrin and structural similarity between two actin-regulating protein families.
Authors: Hatanaka, H. / Ogura, K. / Moriyama, K. / Ichikawa, S. / Yahara, I. / Inagaki, F.
History
DepositionMay 29, 1997Processing site: BNL
Revision 1.0Oct 15, 1997Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 16, 2022Group: Database references / Derived calculations / Other
Category: database_2 / pdbx_database_status ...database_2 / pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: DESTRIN


Theoretical massNumber of molelcules
Total (without water)19,3801
Polymers19,3801
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / 100LEAST FNOE+FREPEL
Representative

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Components

#1: Protein DESTRIN


Mass: 19379.652 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sus scrofa (pig) / Cellular location: CYTOPLASM AND NUCLEUS / Plasmid: PKID-W / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: P60981, UniProt: P60982*PLUS

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
111NOESY
121HN(CO)CA
131HNCA
141CBCA(CO)NH
151CBCANH
161HBHA(CO)NH
171HN(CA)HA
181C(CO)NH
191HC(C)H-TOCSY

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Sample preparation

Sample conditionspH: 7.0 / Temperature: 301 K
Crystal grow
*PLUS
Method: other / Details: NMR

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NMR measurement

NMR spectrometerType: Varian UNITYPLUS 600 / Manufacturer: Varian / Model: UNITYPLUS 600 / Field strength: 600 MHz

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Processing

Software
NameVersionClassification
X-PLOR3.1model building
X-PLOR3.1refinement
X-PLOR3.1phasing
NMR software
NameVersionDeveloperClassification
X-PLOR3.1BRUNGERrefinement
X-PLORstructure solution
RefinementMethod: DYNAMICAL SIMULATED ANNEALING / Software ordinal: 1
Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE.
NMR ensembleConformer selection criteria: LEAST FNOE+FREPEL / Conformers calculated total number: 100 / Conformers submitted total number: 20

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