+Open data
-Basic information
Entry | Database: PDB / ID: 1ak7 | ||||||
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Title | DESTRIN, NMR, 20 STRUCTURES | ||||||
Components | DESTRIN | ||||||
Keywords | ACTIN DEPOLYMERIZATION FACTOR | ||||||
Function / homology | Function and homology information actin filament fragmentation / positive regulation of actin filament depolymerization / actin filament severing / actin filament depolymerization / actin polymerization or depolymerization / cortical actin cytoskeleton / cell motility / actin filament binding / actin cytoskeleton / extracellular exosome / cytoplasm Similarity search - Function | ||||||
Biological species | Sus scrofa (pig) | ||||||
Method | SOLUTION NMR / DYNAMICAL SIMULATED ANNEALING | ||||||
Authors | Hatanaka, H. / Moriyama, K. / Ogura, K. / Ichikawa, S. / Yahara, I. / Inagaki, F. | ||||||
Citation | Journal: Cell(Cambridge,Mass.) / Year: 1996 Title: Tertiary structure of destrin and structural similarity between two actin-regulating protein families. Authors: Hatanaka, H. / Ogura, K. / Moriyama, K. / Ichikawa, S. / Yahara, I. / Inagaki, F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ak7.cif.gz | 1.1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb1ak7.ent.gz | 1 MB | Display | PDB format |
PDBx/mmJSON format | 1ak7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ak/1ak7 ftp://data.pdbj.org/pub/pdb/validation_reports/ak/1ak7 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 19379.652 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sus scrofa (pig) / Cellular location: CYTOPLASM AND NUCLEUS / Plasmid: PKID-W / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: P60981, UniProt: P60982*PLUS |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Sample conditions | pH: 7.0 / Temperature: 301 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian UNITYPLUS 600 / Manufacturer: Varian / Model: UNITYPLUS 600 / Field strength: 600 MHz |
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-Processing
Software |
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NMR software |
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Refinement | Method: DYNAMICAL SIMULATED ANNEALING / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE. | ||||||||||||
NMR ensemble | Conformer selection criteria: LEAST FNOE+FREPEL / Conformers calculated total number: 100 / Conformers submitted total number: 20 |