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- PDB-3iu3: Crystal structure of the Fab fragment of therapeutic antibody Bas... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3iu3 | |||||||||
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Title | Crystal structure of the Fab fragment of therapeutic antibody Basiliximab in complex with IL-2Ra (CD25) ectodomain | |||||||||
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![]() | IMMUNE SYSTEM / IL-2Ra / CD25 / Basiliximab / Simulect / therapeutic antibody / Disulfide bond / Glycoprotein / Membrane / Receptor / Sushi / Transmembrane | |||||||||
Function / homology | ![]() regulation of T cell tolerance induction / interleukin-2 receptor complex / interleukin-2 receptor activity / interleukin-2 binding / regulation of CD4-positive, alpha-beta T cell proliferation / regulation of T cell homeostatic proliferation / IgD immunoglobulin complex / RUNX1 and FOXP3 control the development of regulatory T lymphocytes (Tregs) / IgM immunoglobulin complex / IgA immunoglobulin complex ...regulation of T cell tolerance induction / interleukin-2 receptor complex / interleukin-2 receptor activity / interleukin-2 binding / regulation of CD4-positive, alpha-beta T cell proliferation / regulation of T cell homeostatic proliferation / IgD immunoglobulin complex / RUNX1 and FOXP3 control the development of regulatory T lymphocytes (Tregs) / IgM immunoglobulin complex / IgA immunoglobulin complex / IgE immunoglobulin complex / interleukin-2-mediated signaling pathway / activated T cell proliferation / CD22 mediated BCR regulation / inflammatory response to antigenic stimulus / IgG immunoglobulin complex / Fc epsilon receptor (FCERI) signaling / Interleukin-2 signaling / positive regulation of T cell differentiation / Classical antibody-mediated complement activation / activation-induced cell death of T cells / Initial triggering of complement / positive regulation of activated T cell proliferation / FCGR activation / immunoglobulin mediated immune response / Role of phospholipids in phagocytosis / Role of LAT2/NTAL/LAB on calcium mobilization / Interleukin receptor SHC signaling / Scavenging of heme from plasma / negative regulation of T cell proliferation / Notch signaling pathway / FCERI mediated Ca+2 mobilization / FCGR3A-mediated IL10 synthesis / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / Regulation of Complement cascade / Cell surface interactions at the vascular wall / FCERI mediated MAPK activation / FCGR3A-mediated phagocytosis / antigen binding / B cell receptor signaling pathway / Regulation of actin dynamics for phagocytic cup formation / FCERI mediated NF-kB activation / negative regulation of inflammatory response / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / RAF/MAP kinase cascade / adaptive immune response / Potential therapeutics for SARS / cell surface receptor signaling pathway / blood microparticle / inflammatory response / immune response / external side of plasma membrane / apoptotic process / extracellular space / extracellular exosome / extracellular region / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Du, J. / Yang, H. / Wang, J. / Ding, J. | |||||||||
![]() | ![]() Title: Structural basis for the blockage of IL-2 signaling by therapeutic antibody basiliximab Authors: Du, J. / Yang, H. / Zhang, D. / Wang, J. / Guo, H. / Peng, B. / Guo, Y. / Ding, J. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 639.6 KB | Display | ![]() |
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PDB format | ![]() | 534.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.5 MB | Display | ![]() |
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Full document | ![]() | 1.6 MB | Display | |
Data in XML | ![]() | 56 KB | Display | |
Data in CIF | ![]() | 76 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1mimS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 23144.826 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus, Homo sapiens / Production host: ![]() ![]() #2: Antibody | Mass: 22882.338 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus, Homo sapiens / Production host: ![]() ![]() #3: Protein | Mass: 25388.348 Da / Num. of mol.: 3 Fragment: Extracellular domain, ectodomain, UNP residues 22-238 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #4: Polysaccharide | Source method: isolated from a genetically manipulated source |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.9 Å3/Da / Density % sol: 57.65 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 0.2 M KCl, 0.05 M HEPES pH 7.5, and 45% pentaerythritol propoxylate (5/4 PO/OH), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX225HE / Detector: CCD / Date: Jul 15, 2009 |
Radiation | Monochromator: SAGITALLY FOCUSED Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9796 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→50 Å / Num. all: 57938 / Num. obs: 56779 / % possible obs: 98 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 14.8 % / Biso Wilson estimate: 70.77 Å2 / Rmerge(I) obs: 0.165 / Rsym value: 0.165 / Net I/σ(I): 17 |
Reflection shell | Resolution: 2.9→3 Å / Redundancy: 10 % / Rmerge(I) obs: 0.561 / Mean I/σ(I) obs: 2 / Num. unique all: 5347 / Rsym value: 0.561 / % possible all: 94.5 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1MIM Resolution: 2.9→47.381 Å / SU ML: 2.24 / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Phase error: 28.94 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 39.25 Å2 / ksol: 0.285 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 87.64 Å2
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Refinement step | Cycle: LAST / Resolution: 2.9→47.381 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 19
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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