A: DNA polymerase subunit gamma-1 B: DNA polymerase subunit gamma-2 C: DNA polymerase subunit gamma-2 D: DNA polymerase subunit gamma-1 E: DNA polymerase subunit gamma-2 F: DNA polymerase subunit gamma-2
THE BIOLOGICAL UNIT (HOLOENZYME) CONTAINS ONE MONOMERIC CATALYTIC SUBUNIT AND ONE DIMERIC ACCESSORY SUBUNIT.
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Components
#1: Protein
DNApolymerasesubunitgamma-1 / Mitochondrial DNA polymerase catalytic subunit / PolG-alpha
Mass: 132384.688 Da / Num. of mol.: 2 / Fragment: UNP residues 70-1239 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P54098, DNA-directed DNA polymerase
#2: Protein
DNApolymerasesubunitgamma-2 / Mitochondrial DNA polymerase accessory subunit / PolG-beta / MtPolB / DNA polymerase gamma ...Mitochondrial DNA polymerase accessory subunit / PolG-beta / MtPolB / DNA polymerase gamma accessory 55 kDa subunit / p55
Mass: 48757.902 Da / Num. of mol.: 4 / Fragment: UNP residues 59-485 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9UHN1, DNA-directed DNA polymerase
Sequence details
THE SEQUENCE PROVIDED BY AUTHORS DOES NOT AGREE WITH THE SEQUENCE IN COORDINATES, AND ALSO DOES NOT ...THE SEQUENCE PROVIDED BY AUTHORS DOES NOT AGREE WITH THE SEQUENCE IN COORDINATES, AND ALSO DOES NOT MATCH THE UNIPROT DATABASE. THE FOLLOWING RESIDUES ARE LINKED TOGETHER IN COORDINATES: (A PRO 370) AND (A LYS 371) (B PRO 137) AND (B GLY 179) (C ARG 146) AND (C SER 178) (D ASN 354) AND (D SER 355) (E PRO 137) AND (E GLY 179) (F ARG 146) AND (F SER 178)
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.72 Å3/Da / Density % sol: 54.8 %
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 0.979 Å / Relative weight: 1
Reflection
Resolution: 3.24→47 Å / Num. obs: 76584 / % possible obs: 99 % / Observed criterion σ(I): 2
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Processing
Software
Name
Version
Classification
ADSC
Quantum
datacollection
SOLVE
phasing
CNS
1.2
refinement
HKL-2000
datareduction
HKL-2000
datascaling
Refinement
Method to determine structure: SAD / Resolution: 3.24→46.92 Å / Rfactor Rfree error: 0.007 / Data cutoff high absF: 41884.52 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Details: BULK SOLVENT MODEL USED
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