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- PDB-3hki: Crystal structure of murine thrombin mutant W215A/E217A in comple... -
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Basic information
Entry | Database: PDB / ID: 3hki | ||||||
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Title | Crystal structure of murine thrombin mutant W215A/E217A in complex with the extracellular fragment of human PAR1 | ||||||
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![]() | HYDROLASE / Serine protease / Acute phase / Blood coagulation / Cleavage on pair of basic residues / Disulfide bond / Gamma-carboxyglutamic acid / Glycoprotein / Kringle / Protease / Zymogen / Cell membrane / G-protein coupled receptor / Membrane / Phosphoprotein / Receptor / Transducer / Transmembrane | ||||||
Function / homology | ![]() Common Pathway of Fibrin Clot Formation / Platelet Aggregation (Plug Formation) / negative regulation of renin secretion into blood stream / Gamma-carboxylation of protein precursors / Transport of gamma-carboxylated protein precursors from the endoplasmic reticulum to the Golgi apparatus / Intrinsic Pathway of Fibrin Clot Formation / Removal of aminoterminal propeptides from gamma-carboxylated proteins / dendritic cell homeostasis / negative regulation of glomerular filtration / thrombin-activated receptor signaling pathway ...Common Pathway of Fibrin Clot Formation / Platelet Aggregation (Plug Formation) / negative regulation of renin secretion into blood stream / Gamma-carboxylation of protein precursors / Transport of gamma-carboxylated protein precursors from the endoplasmic reticulum to the Golgi apparatus / Intrinsic Pathway of Fibrin Clot Formation / Removal of aminoterminal propeptides from gamma-carboxylated proteins / dendritic cell homeostasis / negative regulation of glomerular filtration / thrombin-activated receptor signaling pathway / thrombin-activated receptor activity / platelet dense tubular network / cell-cell junction maintenance / Peptide ligand-binding receptors / Thrombin signalling through proteinase activated receptors (PARs) / establishment of synaptic specificity at neuromuscular junction / regulation of interleukin-1 beta production / Regulation of Complement cascade / platelet dense granule organization / connective tissue replacement involved in inflammatory response wound healing / trans-synaptic signaling by endocannabinoid, modulating synaptic transmission / G alpha (q) signalling events / Cell surface interactions at the vascular wall / regulation of sensory perception of pain / positive regulation of smooth muscle contraction / positive regulation of calcium ion transport / : / positive regulation of Rho protein signal transduction / positive regulation of phospholipase C-activating G protein-coupled receptor signaling pathway / cytolysis by host of symbiont cells / thrombospondin receptor activity / thrombin / regulation of blood coagulation / neutrophil-mediated killing of gram-negative bacterium / ligand-gated ion channel signaling pathway / positive regulation of cysteine-type endopeptidase activity involved in apoptotic process / negative regulation of astrocyte differentiation / G-protein alpha-subunit binding / positive regulation of collagen biosynthetic process / negative regulation of cytokine production involved in inflammatory response / anatomical structure morphogenesis / positive regulation of blood coagulation / Common Pathway of Fibrin Clot Formation / release of sequestered calcium ion into cytosol / homeostasis of number of cells within a tissue / positive regulation of vasoconstriction / fibrinolysis / regulation of cytosolic calcium ion concentration / Peptide ligand-binding receptors / positive regulation of release of sequestered calcium ion into cytosol / positive regulation of GTPase activity / caveola / acute-phase response / positive regulation of interleukin-8 production / G protein-coupled receptor activity / negative regulation of proteolysis / lipopolysaccharide binding / positive regulation of receptor signaling pathway via JAK-STAT / regulation of synaptic plasticity / neuromuscular junction / positive regulation of insulin secretion / platelet activation / response to wounding / positive regulation of protein localization to nucleus / antimicrobial humoral immune response mediated by antimicrobial peptide / activation of cysteine-type endopeptidase activity involved in apoptotic process / G-protein beta-subunit binding / positive regulation of interleukin-6 production / positive regulation of reactive oxygen species metabolic process / Thrombin signalling through proteinase activated receptors (PARs) / late endosome / phospholipase C-activating G protein-coupled receptor signaling pathway / peptidase activity / heparin binding / regulation of cell shape / positive regulation of cytosolic calcium ion concentration / positive regulation of cell growth / regulation of gene expression / G alpha (q) signalling events / postsynaptic membrane / positive regulation of canonical NF-kappaB signal transduction / collagen-containing extracellular matrix / endopeptidase activity / negative regulation of neuron apoptotic process / response to lipopolysaccharide / positive regulation of MAPK cascade / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / cell surface receptor signaling pathway / positive regulation of ERK1 and ERK2 cascade / early endosome / positive regulation of cell migration / inflammatory response / positive regulation of protein phosphorylation / G protein-coupled receptor signaling pathway / negative regulation of cell population proliferation / external side of plasma membrane / serine-type endopeptidase activity / signaling receptor binding / calcium ion binding / positive regulation of cell population proliferation Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Gandhi, P.S. / Page, M.J. / Chen, Z. / Bush-Pelc, L. / Di Cera, E. | ||||||
![]() | ![]() Title: Mechanism of the Anticoagulant Activity of Thrombin Mutant W215A/E217A. Authors: Gandhi, P.S. / Page, M.J. / Chen, Z. / Bush-Pelc, L. / Di Cera, E. #1: ![]() Title: The anticoagulant thrombin mutant W215A/E217A has a collapsed primary specificity pocket. Authors: Pineda, A.O. / Chen, Z.W. / Caccia, S. / Cantwell, A.M. / Savvides, S.N. / Waksman, G. / Mathews, F.S. / Di Cera, E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 143.4 KB | Display | ![]() |
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PDB format | ![]() | 111.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 496.7 KB | Display | ![]() |
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Full document | ![]() | 523.3 KB | Display | |
Data in XML | ![]() | 30 KB | Display | |
Data in CIF | ![]() | 41 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3hk3C ![]() 3hk6C ![]() 3hkjC ![]() 1tq0S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Details | THE BIOLOGICAL ASSEMBLY CONSISTS OF A, B AND C CHAINS. |
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Components
#1: Protein/peptide | Mass: 5105.731 Da / Num. of mol.: 2 / Fragment: Light chain: UNP residues 317-360 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein | Mass: 29795.461 Da / Num. of mol.: 2 / Fragment: Heavy chain: UNP residues 361-618 / Mutation: W215A, E217A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #3: Protein/peptide | Mass: 2673.862 Da / Num. of mol.: 2 / Fragment: Extracellular fragment: UNP residues 42-62 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #4: Sugar | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.16 Å3/Da / Density % sol: 61.1 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 100mM Tris-HCl pH 8.5, 20% PEG 10000, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Dec 22, 2008 |
Radiation | Monochromator: Bent Ge(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→40 Å / Num. all: 49562 / Num. obs: 45052 / % possible obs: 90.9 % / Observed criterion σ(I): -3 / Redundancy: 6.9 % / Biso Wilson estimate: 20.2 Å2 / Rmerge(I) obs: 0.051 / Net I/σ(I): 28.6 |
Reflection shell | Resolution: 2.2→2.28 Å / Redundancy: 6.3 % / Rmerge(I) obs: 0.365 / Mean I/σ(I) obs: 4.9 / Num. unique all: 3936 / % possible all: 80.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 1TQ0 Resolution: 2.2→26.23 Å / Rfactor Rfree error: 0.004 / Data cutoff high absF: 150926.62 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 41.1 Å2 | ||||||||||||||||||||||||||||||||||||
Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.2→26.23 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.2→2.34 Å / Rfactor Rfree error: 0.015 / Total num. of bins used: 6
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