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- PDB-3fv3: Secreted aspartic protease 1 from Candida parapsilosis in complex... -

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Basic information

Entry
Database: PDB / ID: 3fv3
TitleSecreted aspartic protease 1 from Candida parapsilosis in complex with pepstatin A
Components
  • Sapp1p-secreted aspartic protease 1
  • pepstatin APepstatin
KeywordsHYDROLASE / pepstatin A / secreted aspartic protease / virulence factor
Function / homology
Function and homology information


candidapepsin / aspartic-type endopeptidase activity / proteolysis / extracellular region
Similarity search - Function
Secreted aspartic endopeptidase / Eukaryotic aspartyl protease / Aspartic peptidase A1 family / Peptidase family A1 domain / Peptidase family A1 domain profile. / Cathepsin D, subunit A; domain 1 / Acid Proteases / Aspartic peptidase, active site / Eukaryotic and viral aspartyl proteases active site. / Aspartic peptidase domain superfamily ...Secreted aspartic endopeptidase / Eukaryotic aspartyl protease / Aspartic peptidase A1 family / Peptidase family A1 domain / Peptidase family A1 domain profile. / Cathepsin D, subunit A; domain 1 / Acid Proteases / Aspartic peptidase, active site / Eukaryotic and viral aspartyl proteases active site. / Aspartic peptidase domain superfamily / Beta Barrel / Mainly Beta
Similarity search - Domain/homology
Pepstatin / Candidapepsin-1
Similarity search - Component
Biological speciesCandida parapsilosis (yeast)
Streptomyces argenteolus subsp. toyonakensis (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.85 Å
AuthorsDostal, J. / Brynda, J. / Hruskova-Heidingsfeldova, O. / Sieglova, I. / Pichova, I. / Rezacova, P.
CitationJournal: J.Struct.Biol. / Year: 2009
Title: The crystal structure of the secreted aspartic protease 1 from Candida parapsilosis in complex with pepstatin A
Authors: Dostal, J. / Brynda, J. / Hruskova-Heidingsfeldova, O. / Sieglova, I. / Pichova, I. / Rezacova, P.
History
DepositionJan 15, 2009Deposition site: RCSB / Processing site: PDBJ
Revision 1.0May 19, 2009Provider: repository / Type: Initial release
Revision 1.1Jul 13, 2011Group: Advisory / Version format compliance
Revision 1.2Feb 27, 2013Group: Other
Revision 1.3Nov 1, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_conn / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_label_asym_id / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id
Revision 1.4Nov 22, 2023Group: Data collection / Category: chem_comp_atom / chem_comp_bond / Item: _chem_comp_atom.atom_id / _chem_comp_bond.atom_id_2

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Sapp1p-secreted aspartic protease 1
I: pepstatin A
B: Sapp1p-secreted aspartic protease 1
J: pepstatin A
C: Sapp1p-secreted aspartic protease 1
K: pepstatin A
D: Sapp1p-secreted aspartic protease 1
L: pepstatin A
E: Sapp1p-secreted aspartic protease 1
M: pepstatin A
F: Sapp1p-secreted aspartic protease 1
N: pepstatin A
G: Sapp1p-secreted aspartic protease 1
O: pepstatin A
H: Sapp1p-secreted aspartic protease 1
P: pepstatin A
hetero molecules


Theoretical massNumber of molelcules
Total (without water)296,15256
Polymers292,40816
Non-polymers3,74340
Water38,8942159
1
B: Sapp1p-secreted aspartic protease 1
J: pepstatin A
hetero molecules


Theoretical massNumber of molelcules
Total (without water)37,0157
Polymers36,5512
Non-polymers4645
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2600 Å2
ΔGint-30 kcal/mol
Surface area13960 Å2
MethodPISA
2
C: Sapp1p-secreted aspartic protease 1
K: pepstatin A
hetero molecules


Theoretical massNumber of molelcules
Total (without water)36,8275
Polymers36,5512
Non-polymers2763
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2050 Å2
ΔGint-12 kcal/mol
Surface area13970 Å2
MethodPISA
3
D: Sapp1p-secreted aspartic protease 1
L: pepstatin A
hetero molecules


Theoretical massNumber of molelcules
Total (without water)37,0197
Polymers36,5512
Non-polymers4685
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2330 Å2
ΔGint-34 kcal/mol
Surface area13970 Å2
MethodPISA
4
E: Sapp1p-secreted aspartic protease 1
M: pepstatin A
hetero molecules


Theoretical massNumber of molelcules
Total (without water)37,1128
Polymers36,5512
Non-polymers5616
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2660 Å2
ΔGint-28 kcal/mol
Surface area13910 Å2
MethodPISA
5
F: Sapp1p-secreted aspartic protease 1
N: pepstatin A
hetero molecules


Theoretical massNumber of molelcules
Total (without water)36,8355
Polymers36,5512
Non-polymers2843
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1790 Å2
ΔGint-27 kcal/mol
Surface area14090 Å2
MethodPISA
6
G: Sapp1p-secreted aspartic protease 1
O: pepstatin A
hetero molecules


Theoretical massNumber of molelcules
Total (without water)37,2169
Polymers36,5512
Non-polymers6657
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2500 Å2
ΔGint-60 kcal/mol
Surface area13990 Å2
MethodPISA
7
H: Sapp1p-secreted aspartic protease 1
P: pepstatin A
hetero molecules


Theoretical massNumber of molelcules
Total (without water)37,2049
Polymers36,5512
Non-polymers6537
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2870 Å2
ΔGint-30 kcal/mol
Surface area13880 Å2
MethodPISA
8
A: Sapp1p-secreted aspartic protease 1
I: pepstatin A
hetero molecules


Theoretical massNumber of molelcules
Total (without water)36,9236
Polymers36,5512
Non-polymers3724
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2200 Å2
ΔGint-20 kcal/mol
Surface area13930 Å2
MethodPISA
Unit cell
Length a, b, c (Å)86.488, 194.247, 97.147
Angle α, β, γ (deg.)90.00, 91.52, 90.00
Int Tables number4
Space group name H-MP1211

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Components

#1: Protein
Sapp1p-secreted aspartic protease 1


Mass: 35865.160 Da / Num. of mol.: 8 / Source method: isolated from a natural source / Details: gene sapp1 / Source: (natural) Candida parapsilosis (yeast) / Strain: P-69 / References: UniProt: P32951*PLUS, candidapepsin
#2: Protein/peptide
pepstatin A / Pepstatin /


Type: Oligopeptide / Class: Enzyme inhibitor / Mass: 685.891 Da / Num. of mol.: 8 / Source method: obtained synthetically / Details: Synthetic peptide
Source: (synth.) Streptomyces argenteolus subsp. toyonakensis (bacteria)
References: Pepstatin
#3: Chemical
ChemComp-SO4 / SULFATE ION / Sulfate


Mass: 96.063 Da / Num. of mol.: 15 / Source method: obtained synthetically / Formula: SO4
#4: Chemical...
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL / Glycerol


Mass: 92.094 Da / Num. of mol.: 25 / Source method: obtained synthetically / Formula: C3H8O3
#5: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 2159 / Source method: isolated from a natural source / Formula: H2O
Sequence detailsTHE SEQUENCE OF ENTITY 1 HAS BEEN DEPOSITED TO THE NCBI DATABASE WITH ACCESSION NUMBER FL560879.

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.79 Å3/Da / Density % sol: 55.91 %
Crystal growTemperature: 292 K / Method: vapor diffusion, hanging drop / pH: 7
Details: five-fold molar inhibitor excess, Cpr=8mg/ml; drops: 0.002ml protein + 0.001ml reservoir + 0.0002ml 10mM ZnAc; reservoir: 0.1M Tris pH 7.0, 2.0M Ammonium Sulfate, 10% glycerol, VAPOR ...Details: five-fold molar inhibitor excess, Cpr=8mg/ml; drops: 0.002ml protein + 0.001ml reservoir + 0.0002ml 10mM ZnAc; reservoir: 0.1M Tris pH 7.0, 2.0M Ammonium Sulfate, 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 292K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 19-BM / Wavelength: 0.979 Å
DetectorType: SBC-3 / Detector: CCD / Date: Jul 10, 2008
RadiationMonochromator: double crystal monochromator Si 111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.979 Å / Relative weight: 1
ReflectionResolution: 1.85→50 Å / Num. all: 274936 / Num. obs: 273011 / % possible obs: 99.3 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.8 % / Rmerge(I) obs: 0.045 / Net I/σ(I): 27.2
Reflection shellResolution: 1.85→1.9 Å / Redundancy: 3.8 % / Rmerge(I) obs: 0.49 / Mean I/σ(I) obs: 2.4 / % possible all: 98.5

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Processing

Software
NameVersionClassification
HKL-2000data collection
MOLREPphasing
REFMAC5.3.0037refinement
HKL-3000data reduction
HKL-3000data scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 1EAG
Resolution: 1.85→46.03 Å / Cor.coef. Fo:Fc: 0.968 / Cor.coef. Fo:Fc free: 0.961 / SU B: 4.955 / SU ML: 0.08 / TLS residual ADP flag: LIKELY RESIDUAL / Cross valid method: THROUGHOUT / ESU R: 0.113 / ESU R Free: 0.104 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
RfactorNum. reflection% reflectionSelection details
Rfree0.19014 1368 0.5 %RANDOM
Rwork0.16634 ---
obs0.16646 269660 99.99 %-
all-252690 --
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK
Displacement parametersBiso mean: 15.135 Å2
Baniso -1Baniso -2Baniso -3
1--2.83 Å20 Å20.37 Å2
2--0.57 Å20 Å2
3---2.27 Å2
Refinement stepCycle: LAST / Resolution: 1.85→46.03 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms20582 0 225 2159 22966
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0120.02221822
X-RAY DIFFRACTIONr_bond_other_d
X-RAY DIFFRACTIONr_angle_refined_deg1.2561.96829913
X-RAY DIFFRACTIONr_angle_other_deg
X-RAY DIFFRACTIONr_dihedral_angle_1_deg6.01552956
X-RAY DIFFRACTIONr_dihedral_angle_2_deg34.70125.773899
X-RAY DIFFRACTIONr_dihedral_angle_3_deg12.185153348
X-RAY DIFFRACTIONr_dihedral_angle_4_deg16.491558
X-RAY DIFFRACTIONr_chiral_restr0.0870.23531
X-RAY DIFFRACTIONr_gen_planes_refined0.0050.0216494
X-RAY DIFFRACTIONr_gen_planes_other
X-RAY DIFFRACTIONr_nbd_refined0.1920.210161
X-RAY DIFFRACTIONr_nbd_other
X-RAY DIFFRACTIONr_nbtor_refined0.3020.215126
X-RAY DIFFRACTIONr_nbtor_other
X-RAY DIFFRACTIONr_xyhbond_nbd_refined0.1270.22145
X-RAY DIFFRACTIONr_xyhbond_nbd_other
X-RAY DIFFRACTIONr_metal_ion_refined
X-RAY DIFFRACTIONr_metal_ion_other
X-RAY DIFFRACTIONr_symmetry_vdw_refined0.1820.2236
X-RAY DIFFRACTIONr_symmetry_vdw_other
X-RAY DIFFRACTIONr_symmetry_hbond_refined0.1380.2109
X-RAY DIFFRACTIONr_symmetry_hbond_other
X-RAY DIFFRACTIONr_symmetry_metal_ion_refined
X-RAY DIFFRACTIONr_symmetry_metal_ion_other
X-RAY DIFFRACTIONr_mcbond_it0.611.514075
X-RAY DIFFRACTIONr_mcbond_other
X-RAY DIFFRACTIONr_mcangle_it1.023222372
X-RAY DIFFRACTIONr_scbond_it1.63538740
X-RAY DIFFRACTIONr_scangle_it2.5444.57415
X-RAY DIFFRACTIONr_rigid_bond_restr
X-RAY DIFFRACTIONr_sphericity_free
X-RAY DIFFRACTIONr_sphericity_bonded
LS refinement shellResolution: 1.846→1.894 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.288 94 -
Rwork0.241 19264 -
obs--99.94 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
12.4151-0.6582-0.20851.58280.25451.42410.12470.36490.2977-0.5216-0.038-0.2203-0.1451-0.0628-0.08670.2275-0.03310.12420.06690.06750.022324.6051.0090.558
22.62410.45210.07812.19090.47281.3305-0.0283-0.07350.1224-0.29270.1233-0.39770.02640.2877-0.0950.0682-0.00860.13050.01630.01120.075835.549-5.6029.422
32.8072-1.4608-1.42351.52440.76021.3947-0.1941-0.70990.07520.20560.2123-0.21550.23260.2962-0.01810.07390.00430.0030.2086-0.028-0.024723.824-2.75529.843
47.4608-2.1132-1.26661.97760.93342.6222-0.0412-0.65580.73850.07250.1815-0.3888-0.11920.2149-0.14030.0189-0.03060.01960.1358-0.09330.006625.1525.31629.502
52.5672-0.0587-0.22251.30380.35771.42520.0279-0.36610.2806-0.06940.1473-0.60430.02530.4414-0.17530.02-0.01990.07520.1372-0.05850.202739.805-0.7917.88
61.82180.3584-0.09531.4806-0.00430.67410.07170.0353-0.30280.06-0.092-0.06110.1649-0.01130.02030.0780.0253-0.015-0.0056-0.0377-0.042322.9558.99159.46
72.11370.5091-0.85231.6985-0.68332.23840.1172-0.102-0.13060.1078-0.0828-0.2170.1102-0.0294-0.03440.02940.0283-0.03870.00320.001-0.06933.86916.40764.333
82.08240.25760.62031.33130.14991.24030.07120.02240.37020.0007-0.11750.0705-0.159-0.04240.04640.030.02970.0239-0.0133-0.0232-0.016625.74537.31861.074
94.572-0.49840.3841.6062-0.10452.40580.09870.55370.182-0.2641-0.16390.057-0.2047-0.14480.06520.0680.07720.01460.00040.0033-0.061525.38737.2251.809
101.69520.2391-0.73330.98790.31740.53150.1281-0.0187-0.00970.0435-0.137-0.26380.03910.1420.0090.01380.0320.00080.00070.0123-0.019839.43325.25759.468
1111.4865-9.4576-3.75712.11952.87213.7850.35120.48850.432-0.3016-0.2513-0.57030.0009-0.0415-0.09980.0390.05420.06680.04850.01810.132913.218103.384.264
125.2794-0.61310.19941.53350.49221.31460.2037-0.0094-0.55850.105-0.0998-0.09870.4429-0.0419-0.10390.18580.0396-0.01160.05330.02350.02072.40291.47686.893
135.6735-0.82-0.18052.41380.13942.30450.1768-0.61450.36210.22870.0646-0.47410.2860.1735-0.24130.10640.0697-0.05730.143-0.02360.085115.52998.99994.983
145.0634-2.23720.41393.8020.78541.54420.1385-0.76731.24950.2509-0.0162-0.2345-0.16980.0487-0.12220.121-0.00610.09820.2133-0.23430.39491.328119.013100.13
156.4141-1.5819-0.16512.32220.54991.45470.2148-0.48741.46860.0663-0.0545-0.6725-0.03360.0963-0.16020.05760.02340.06720.0875-0.14810.451812.596114.14194.609
163.8747-0.04060.83371.2375-0.50471.3777-0.24890.16790.63680.11910.0820.0417-0.3656-0.08140.16690.09970.039-0.07160.02040.0313-0.014981.19974.00738.117
172.8807-0.12691.1011.9881-0.05481.8192-0.1762-0.46470.20610.21850.18480.1822-0.3068-0.3668-0.00850.06680.1390.01560.1626-0.0317-0.048770.90469.3548.954
182.02530.5843-0.89210.55990.44811.8453-0.0086-0.2754-0.24490.3184-0.0434-0.04140.1997-0.02080.0520.11860.03810.00260.10790.0617-0.055680.17349.29255.65
195.45460.1479-0.69941.65610.11523.5741-0.01980.2329-0.41890.008-0.0735-0.06450.25190.08520.09340.08610.0264-0.0482-0.00550.0204-0.059481.68946.36147.995
202.712-0.80150.3861.5472-0.77091.0239-0.0219-0.3297-0.27270.12370.13450.5277-0.1047-0.3544-0.11250.0240.05560.01720.16360.05580.043665.65359.19547.471
211.7082-0.2365-0.1560.6811-0.25620.91520.0019-0.2452-0.25750.02430.02150.06960.0290.0403-0.02350.01770.0171-0.0250.03140.0409-0.08664.686146.55113.876
222.309-0.3311-0.83260.91510.42232.65470.0404-0.2625-0.02270.053-0.0746-0.0466-0.09230.09240.0342-0.00550.0014-0.0392-0.0045-0.0048-0.13615.452155.32311.325
231.90390.36890.52830.30690.06720.7454-0.03320.16380.2607-0.0317-0.0088-0.0119-0.18630.01220.0420.05820.0159-0.0059-0.00720.019-0.09676.301166.254-6.304
244.5450.560.9820.80270.23391.9370.010.3938-0.0888-0.08220.0182-0.00430.02870.0009-0.02810.02730.0173-0.0051-0.0210.0013-0.17586.064158.884-12.574
252.1336-0.2521-0.16720.8807-0.02790.48120.0397-0.04160.05840.0684-0.0296-0.11-0.0570.2492-0.01020.0057-0.0059-0.01920.0269-0.0059-0.126620.317157.7731.105
269.0511-3.3429-2.6816.7591.60993.73820.16770.37010.2642-0.2870.0318-0.0596-0.1339-0.0215-0.1995-0.02130.0305-0.02630.0009-0.0058-0.141651.288149.0751.438
271.6504-0.0264-0.02661.0608-0.12290.90170.02550.07460.22580.0881-0.0559-0.0117-0.16620.01970.03050.04420.0179-0.0151-0.02650.0175-0.089562.66157.4399.343
281.7679-0.00950.35441.44080.24821.56850.0318-0.0156-0.02140.07340.00170.1348-0.0671-0.0565-0.0334-0.00650.02870.0131-0.0243-0.0049-0.142350.133146.78113.1
291.99810.1929-1.12833.43090.04752.0138-0.05420.077-0.5114-0.2843-0.0657-0.42490.37060.1710.11990.07740.0791-0.00580.0111-0.03180.060162.774126.9095.417
301.5224-0.157-0.091.4431-0.24791.55190.08140.1801-0.277-0.1132-0.06170.04180.1664-0.0468-0.01970.00890.0233-0.03-0.0137-0.0502-0.069251.687134.3594.025
312.26020.13970.40530.71430.2930.78430.0365-0.29080.51540.1069-0.04840.04-0.0213-0.00940.01190.053-0.00070.0372-0.0131-0.09970.053179.78820.59863.258
322.10290.00890.34060.7366-0.11941.22580.0836-0.23220.17720.1092-0.0780.05220.0362-0.047-0.00550.0259-0.0150.0346-0.0438-0.0284-0.073969.7258.98959.5
332.94971.0525-0.81670.3858-0.20021.0359-0.07560.3905-0.2881-0.00220.0561-0.09720.16030.03910.01960.04280.03340.00750.0138-0.0457-0.055780.7221.0140.403
345.97691.3356-1.25560.588-0.07411.8641-0.03250.63720.2706-0.06190.14980.0107-0.11490.0688-0.11730.01340.02020.01430.02190.0128-0.100979.7127.99536.38
352.1450.11360.11610.76670.27510.84290.08210.0750.18690.0539-0.040.10470.0004-0.169-0.04220.00120.00570.0263-0.04880.0041-0.056164.6329.21949.041
361.80670.03850.15290.9050.00261.54770.02940.1108-0.1481-0.1749-0.04680.08520.0289-0.01780.01730.05480.0004-0.0659-0.0163-0.0566-0.076659.488165.17347.129
371.55810.21330.05241.2885-0.12881.0873-0.0169-0.15910.0265-0.0768-0.03440.1888-0.0765-0.29380.0514-0.00660.0236-0.06560.0297-0.0702-0.04449.95173.54656.188
381.4867-0.74420.81550.7376-0.15611.4284-0.0911-0.39890.00750.16370.02630.1373-0.1255-0.2690.06470.04690.0007-0.00480.1428-0.0378-0.096559.672169.50476.947
395.1118-1.38811.08921.5207-0.50922.6118-0.0253-0.4723-0.3470.14720.03770.19610.2149-0.3194-0.01230.0186-0.04020.00520.11210.0089-0.11658.16161.4176.535
401.65130.06120.29640.75240.13481.3675-0.0105-0.36230.00040.0334-0.01390.2925-0.0348-0.55460.0245-0.0144-0.0052-0.02430.1776-0.0551-0.000743.862168.21564.653
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1A1 - 128
2X-RAY DIFFRACTION2A129 - 194
3X-RAY DIFFRACTION3A195 - 249
4X-RAY DIFFRACTION4A250 - 301
5X-RAY DIFFRACTION5A302 - 339
6X-RAY DIFFRACTION6B1 - 128
7X-RAY DIFFRACTION7B129 - 185
8X-RAY DIFFRACTION8B186 - 249
9X-RAY DIFFRACTION9B250 - 302
10X-RAY DIFFRACTION10B303 - 339
11X-RAY DIFFRACTION11C1 - 14
12X-RAY DIFFRACTION12C15 - 129
13X-RAY DIFFRACTION13C130 - 194
14X-RAY DIFFRACTION14C195 - 262
15X-RAY DIFFRACTION15C263 - 339
16X-RAY DIFFRACTION16D1 - 120
17X-RAY DIFFRACTION17D121 - 194
18X-RAY DIFFRACTION18D195 - 246
19X-RAY DIFFRACTION19D247 - 301
20X-RAY DIFFRACTION20D302 - 339
21X-RAY DIFFRACTION21E1 - 128
22X-RAY DIFFRACTION22E129 - 185
23X-RAY DIFFRACTION23E186 - 249
24X-RAY DIFFRACTION24E250 - 301
25X-RAY DIFFRACTION25E302 - 339
26X-RAY DIFFRACTION26F1 - 14
27X-RAY DIFFRACTION27F15 - 128
28X-RAY DIFFRACTION28F129 - 194
29X-RAY DIFFRACTION29F195 - 262
30X-RAY DIFFRACTION30F263 - 339
31X-RAY DIFFRACTION31G1 - 118
32X-RAY DIFFRACTION32G119 - 194
33X-RAY DIFFRACTION33G195 - 249
34X-RAY DIFFRACTION34G250 - 301
35X-RAY DIFFRACTION35G302 - 339
36X-RAY DIFFRACTION36H1 - 118
37X-RAY DIFFRACTION37H119 - 194
38X-RAY DIFFRACTION38H195 - 249
39X-RAY DIFFRACTION39H250 - 301
40X-RAY DIFFRACTION40H302 - 339

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