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Yorodumi- PDB-3fs3: Crystal structure of malaria parasite Nucleosome Assembly Protein... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3fs3 | ||||||
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Title | Crystal structure of malaria parasite Nucleosome Assembly Protein (NAP) | ||||||
Components | Nucleosome assembly protein 1, putative | ||||||
Keywords | CHAPERONE / plasmodium falciparum / nucleosome assembly protein / protein localization / histone recognition / structural analysis | ||||||
Function / homology | Function and homology information nucleosome assembly / histone binding / chromatin binding / chromatin / protein kinase binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | Plasmodium falciparum (malaria parasite P. falciparum) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.3 Å | ||||||
Authors | Gill, J. / Yogavel, M. / Kumar, A. / Belrhali, H. / Sharma, A. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2009 Title: Crystal structure of malaria parasite nucleosome assembly protein: distinct modes of protein localization and histone recognition. Authors: Gill, J. / Yogavel, M. / Kumar, A. / Belrhali, H. / Jain, S.K. / Rug, M. / Brown, M. / Maier, A.G. / Sharma, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3fs3.cif.gz | 57.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3fs3.ent.gz | 40 KB | Display | PDB format |
PDBx/mmJSON format | 3fs3.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fs/3fs3 ftp://data.pdbj.org/pub/pdb/validation_reports/fs/3fs3 | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 42048.484 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Plasmodium falciparum (malaria parasite P. falciparum) Strain: 3D7 / Gene: PFL0185c / Plasmid: pET28a / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: Q8I608 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 42.5 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.6 Details: 20% PEG 3350, 0.2M magnesium chloride, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: BM14 / Wavelength: 1 Å |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Jun 15, 2006 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→50 Å / Num. obs: 12065 / Observed criterion σ(I): 2 / Redundancy: 5.1 % / Rmerge(I) obs: 0.052 / Net I/σ(I): 26.1 |
Reflection shell | Resolution: 2.3→2.38 Å / Redundancy: 5.1 % / Rmerge(I) obs: 0.238 / Mean I/σ(I) obs: 4.98 / Num. unique all: 1217 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 2.3→50 Å
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Refinement step | Cycle: LAST / Resolution: 2.3→50 Å
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