transcription cis-regulatory region binding / DNA-binding transcription factor activity / response to antibiotic / negative regulation of DNA-templated transcription / metal ion binding Similarity search - Function
A: Tetracycline repressor protein class B from transposon Tn10, Tetracycline repressor protein class D B: Tetracycline repressor protein class B from transposon Tn10, Tetracycline repressor protein class D
Mass: 23448.801 Da / Num. of mol.: 2 Fragment: DNA-binding domain (residues 1-50) and the effector-binding domain (residues 51-208) Mutation: H64K, S135L, S138I Source method: isolated from a genetically manipulated source Details: THE FUSION PROTEIN OF DNA-BINDING DOMAIN (RESIDUES 1-50) FROM TETR VARIANT B AND THE EFFECTOR-BINDING DOMAIN (RESIDUES 51-208) FROM TETR VARIANT D Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K12 / Gene: tetR / Plasmid: pWH610 / Production host: Escherichia coli (E. coli) / Strain (production host): RB791 / References: UniProt: P04483, UniProt: P0ACT4
Mass: 18.015 Da / Num. of mol.: 116 / Source method: isolated from a natural source / Formula: H2O
Sequence details
THE FUSION PROTEIN OF DNA-BINDING DOMAIN (RESIDUES 1-50) FROM TETR VARIANT B AND THE EFFECTOR- ...THE FUSION PROTEIN OF DNA-BINDING DOMAIN (RESIDUES 1-50) FROM TETR VARIANT B AND THE EFFECTOR-BINDING DOMAIN (RESIDUES 51-208) FROM TETR VARIANT D
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.43 Å3/Da / Density % sol: 49.44 %
Crystal grow
Temperature: 292 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 1M dipotassium hydrogen phosphate, 200mM sodium chloride, 50mM Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K
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