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Yorodumi- PDB-2tct: THE COMPLEX FORMED BETWEEN TET REPRESSOR AND TETRACYCLINE-MG2+ RE... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2tct | |||||||||
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Title | THE COMPLEX FORMED BETWEEN TET REPRESSOR AND TETRACYCLINE-MG2+ REVEALS MECHANISM OF ANTIBIOTIC RESISTANCE | |||||||||
Components | TETRACYCLINE REPRESSOR | |||||||||
Keywords | TRANSCRIPTION REGULATION | |||||||||
Function / homology | Function and homology information transcription cis-regulatory region binding / DNA-binding transcription factor activity / response to antibiotic / negative regulation of DNA-templated transcription / metal ion binding Similarity search - Function | |||||||||
Biological species | Escherichia coli (E. coli) | |||||||||
Method | X-RAY DIFFRACTION / Resolution: 2.1 Å | |||||||||
Authors | Hinrichs, W. / Kisker, C. / Saenger, W. | |||||||||
Citation | Journal: J.Mol.Biol. / Year: 1995 Title: The complex formed between Tet repressor and tetracycline-Mg2+ reveals mechanism of antibiotic resistance. Authors: Kisker, C. / Hinrichs, W. / Tovar, K. / Hillen, W. / Saenger, W. #1: Journal: Science / Year: 1994 Title: Structure of the Tet Repressor-Tetracycline Complex and Regulation of Antibiotic Resistance Authors: Hinrichs, W. / Kisker, C. / Duevel, M. / Mueller, A. / Tovar, K. / Hillen, W. / Saenger, W. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2tct.cif.gz | 55.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2tct.ent.gz | 40.5 KB | Display | PDB format |
PDBx/mmJSON format | 2tct.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2tct_validation.pdf.gz | 461.7 KB | Display | wwPDB validaton report |
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Full document | 2tct_full_validation.pdf.gz | 468.7 KB | Display | |
Data in XML | 2tct_validation.xml.gz | 6.5 KB | Display | |
Data in CIF | 2tct_validation.cif.gz | 9.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/tc/2tct ftp://data.pdbj.org/pub/pdb/validation_reports/tc/2tct | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 23272.334 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) Description: REMAULT E.,STANSSENS P.,FIERS W. PLASMID VECTORS FOR HIGH-EFFICIENCY EXPRESSION CONTROLLED BY THE PL PROMOTER OF COLIPHAGE LAMBDA. GENE 15, 81-93 (1981). Plasmid: PWH904 (K. TOVAR, PH.D. THESIS, UNIV. ERLANGEN-NUERNBERG, 1989) Production host: Escherichia coli (E. coli) / Strain (production host): K12 DELTA H1 DELTA TRP / References: UniProt: P0ACT4 |
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#2: Chemical | ChemComp-MG / |
#3: Chemical | ChemComp-CTC / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 46.63 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal | *PLUS Density % sol: 42 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 ℃ / pH: 7.2 / Method: vapor diffusion | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | Resolution: 2→20 Å / Num. obs: 14105 / % possible obs: 92.6 % / Observed criterion σ(I): 0 |
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-Processing
Software |
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Refinement | Resolution: 2.1→10 Å / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 2.1→10 Å
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Refine LS restraints |
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Refinement | *PLUS Num. reflection all: 12229 / Num. reflection obs: 10754 / Rfactor all: 0.183 / Rfactor obs: 0.173 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 22.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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