[English] 日本語
![](img/lk-miru.gif)
- PDB-2tct: THE COMPLEX FORMED BETWEEN TET REPRESSOR AND TETRACYCLINE-MG2+ RE... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 2tct | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | THE COMPLEX FORMED BETWEEN TET REPRESSOR AND TETRACYCLINE-MG2+ REVEALS MECHANISM OF ANTIBIOTIC RESISTANCE | |||||||||
![]() | TETRACYCLINE REPRESSOR | |||||||||
![]() | TRANSCRIPTION REGULATION | |||||||||
Function / homology | ![]() transcription cis-regulatory region binding / DNA-binding transcription factor activity / response to antibiotic / negative regulation of DNA-templated transcription / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() | |||||||||
![]() | Hinrichs, W. / Kisker, C. / Saenger, W. | |||||||||
![]() | ![]() Title: The complex formed between Tet repressor and tetracycline-Mg2+ reveals mechanism of antibiotic resistance. Authors: Kisker, C. / Hinrichs, W. / Tovar, K. / Hillen, W. / Saenger, W. #1: ![]() Title: Structure of the Tet Repressor-Tetracycline Complex and Regulation of Antibiotic Resistance Authors: Hinrichs, W. / Kisker, C. / Duevel, M. / Mueller, A. / Tovar, K. / Hillen, W. / Saenger, W. | |||||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 55.9 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 40.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 461.7 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 468.7 KB | Display | |
Data in XML | ![]() | 6.5 KB | Display | |
Data in CIF | ![]() | 9.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
Unit cell |
| ||||||||
Components on special symmetry positions |
|
-
Components
#1: Protein | Mass: 23272.334 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Description: REMAULT E.,STANSSENS P.,FIERS W. PLASMID VECTORS FOR HIGH-EFFICIENCY EXPRESSION CONTROLLED BY THE PL PROMOTER OF COLIPHAGE LAMBDA. GENE 15, 81-93 (1981). Plasmid: PWH904 (K. TOVAR, PH.D. THESIS, UNIV. ERLANGEN-NUERNBERG, 1989) Production host: ![]() ![]() |
---|---|
#2: Chemical | ChemComp-MG / |
#3: Chemical | ChemComp-CTC / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 46.63 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal | *PLUS Density % sol: 42 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 ℃ / pH: 7.2 / Method: vapor diffusion | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Reflection | Resolution: 2→20 Å / Num. obs: 14105 / % possible obs: 92.6 % / Observed criterion σ(I): 0 |
---|
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 2.1→10 Å / σ(F): 0
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→10 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Num. reflection all: 12229 / Num. reflection obs: 10754 / Rfactor all: 0.183 / Rfactor obs: 0.173 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 22.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
|