[English] 日本語
![](img/lk-miru.gif)
- PDB-3fcy: Crystal Structure of Acetyl Xylan Esterase 1 from Thermoanaerobac... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 3fcy | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal Structure of Acetyl Xylan Esterase 1 from Thermoanaerobacterium sp. JW/SL YS485 | ||||||
![]() | Xylan esterase 1 | ||||||
![]() | HYDROLASE / alpha/beta hydrolase / carbohydrate esterase / CE7 / Thermoanaerobacterium sp. | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
![]() | Krastanova, I. / Cassetta, A. / Lamba, D. | ||||||
![]() | ![]() Title: Crystal Structure Analysis of Acetyl Xylan Esterase 1 from Thermoanaerobacterium sp. JW/SL YS485 Authors: Krastanova, I. / Cassetta, A. / Wiegel, J. / Lamba, D. #1: ![]() Title: Purification and Characterization of Two Thermostable Acetyl Xylan Esterases from Thermoanaerobacterium sp. Strain JW/SL-YS485 Authors: Shao, W. / Wiegel, J. #2: ![]() Title: Isolation, Analysis, and Expression of Two Genes from Thermoanaerobacterium sp. Strain JW/SL YS485: a beta-Xylosidase and a Novel Acetyl Xylan Esterase with Cephalosporin C Deacetylase Activity Authors: Lorenz, W.W. / Wiegel, J. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 205.3 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 162.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 451.2 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 458.5 KB | Display | |
Data in XML | ![]() | 39 KB | Display | |
Data in CIF | ![]() | 55.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3fvrS S: Starting model for refinement |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
Unit cell |
| ||||||||
Components on special symmetry positions |
|
-
Components
#1: Protein | Mass: 39371.500 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: JW/SL YS485 / Gene: axe1 / Plasmid: modified pET / Production host: ![]() ![]() References: UniProt: O30361, acetylxylan esterase, cephalosporin-C deacetylase #2: Chemical | #3: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.41 Å3/Da / Density % sol: 49.04 % |
---|---|
Crystal grow | Temperature: 293 K / Method: microbatch / pH: 7.5 Details: 14% PEG 400, 0.1M calcium chloride, 0.05M sodium HEPES, pH 7.5, microbatch, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Sep 16, 2005 |
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9786 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→25 Å / Num. all: 66742 / Num. obs: 66742 / % possible obs: 100 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 11.1 % / Biso Wilson estimate: 27.56 Å2 / Rmerge(I) obs: 0.067 / Χ2: 1.01 |
Reflection shell | Resolution: 2.1→2.14 Å / Redundancy: 10.6 % / Rmerge(I) obs: 0.329 / Mean I/σ(I) obs: 2.3 / Num. unique all: 3296 / Χ2: 0.972 / % possible all: 100 |
-Phasing
Phasing | Method: ![]() | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Phasing MR |
|
-
Processing
Software |
| ||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: PDB entry 3FVR Resolution: 2.1→25 Å / Occupancy max: 1 / Occupancy min: 1 / FOM work R set: 0.864 / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
| ||||||||||||||||||||||||||||
Solvent computation | Bsol: 53.972 Å2 | ||||||||||||||||||||||||||||
Displacement parameters | Biso max: 59.24 Å2 / Biso mean: 28 Å2 / Biso min: 11.85 Å2
| ||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→25 Å
| ||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||
Xplor file |
|