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- PDB-5dlq: Crystal structure of RanGTP-Exportin 4-eIF5A complex -

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Basic information

Entry
Database: PDB / ID: 5dlq
TitleCrystal structure of RanGTP-Exportin 4-eIF5A complex
Components
  • Eukaryotic translation initiation factor 5A-1
  • Exportin-4
  • GTP-binding nuclear protein Ran
KeywordsPROTEIN TRANSPORT / Active transport / Nuclear transport / Nuclear export Importin-Beta family / Exportin / HEAT repeat / GTPase / Nucleotide binding / eIF5A / Translation factor / Hypusine / Unusual amino acid
Function / homology
Function and homology information


Hypusine synthesis from eIF5A-lysine / positive regulation of translational termination / annulate lamellae / positive regulation of translational elongation / pre-miRNA export from nucleus / RNA nuclear export complex / snRNA import into nucleus / nuclear export signal receptor activity / Regulation of cholesterol biosynthesis by SREBP (SREBF) / protein localization to nucleolus ...Hypusine synthesis from eIF5A-lysine / positive regulation of translational termination / annulate lamellae / positive regulation of translational elongation / pre-miRNA export from nucleus / RNA nuclear export complex / snRNA import into nucleus / nuclear export signal receptor activity / Regulation of cholesterol biosynthesis by SREBP (SREBF) / protein localization to nucleolus / Rev-mediated nuclear export of HIV RNA / Nuclear import of Rev protein / NEP/NS2 Interacts with the Cellular Export Machinery / GTP metabolic process / tRNA processing in the nucleus / Postmitotic nuclear pore complex (NPC) reformation / MicroRNA (miRNA) biogenesis / DNA metabolic process / mitotic sister chromatid segregation / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator / translational elongation / ribosomal large subunit export from nucleus / viral process / translation elongation factor activity / U6 snRNA binding / nuclear pore / ribosomal subunit export from nucleus / ribosomal small subunit export from nucleus / tumor necrosis factor-mediated signaling pathway / centriole / protein export from nucleus / mitotic spindle organization / Transcriptional regulation by small RNAs / recycling endosome / cellular response to virus / positive regulation of protein import into nucleus / protein import into nucleus / GDP binding / positive regulation of protein binding / nuclear envelope / melanosome / protein transport / mitotic cell cycle / ribosome binding / G protein activity / midbody / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / cadherin binding / protein heterodimerization activity / cell division / GTPase activity / chromatin binding / endoplasmic reticulum membrane / GTP binding / chromatin / nucleolus / magnesium ion binding / positive regulation of transcription by RNA polymerase II / protein-containing complex / RNA binding / extracellular exosome / nucleoplasm / nucleus / membrane / cytosol / cytoplasm
Similarity search - Function
Exportin 4/7-like / Translation elongation factor, IF5A, hypusine site / Eukaryotic initiation factor 5A hypusine signature. / Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold / : / Translation initiation factor 5A-like, N-terminal / Translation elongation factor, IF5A C-terminal / Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold / Translation elongation factor IF5A-like / Exportin-1, C-terminal ...Exportin 4/7-like / Translation elongation factor, IF5A, hypusine site / Eukaryotic initiation factor 5A hypusine signature. / Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold / : / Translation initiation factor 5A-like, N-terminal / Translation elongation factor, IF5A C-terminal / Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold / Translation elongation factor IF5A-like / Exportin-1, C-terminal / CRM1 C terminal / small GTPase Ran family profile. / Ran GTPase / SH3 type barrels. - #30 / Leucine-rich Repeat Variant / Leucine-rich Repeat Variant / Ran (Ras-related nuclear proteins) /TC4 subfamily of small GTPases / Nucleic acid-binding proteins / Rho (Ras homology) subfamily of Ras-like small GTPases / Ras subfamily of RAS small GTPases / Small GTPase / Ras family / Rab subfamily of small GTPases / SH3 type barrels. / Armadillo-like helical / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / Small GTP-binding protein domain / Alpha Horseshoe / Translation protein SH3-like domain superfamily / Armadillo-type fold / Ribosomal protein L2, domain 2 / Roll / P-loop containing nucleotide triphosphate hydrolases / Nucleic acid-binding, OB-fold / Beta Barrel / Rossmann fold / P-loop containing nucleoside triphosphate hydrolase / 3-Layer(aba) Sandwich / Mainly Beta / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
GUANOSINE-5'-TRIPHOSPHATE / GTP-binding nuclear protein Ran / Eukaryotic translation initiation factor 5A-1 / Exportin-4
Similarity search - Component
Biological speciesMus musculus (house mouse)
Homo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 3.2 Å
AuthorsAksu, M. / Trakhanov, S. / Gorlich, D.
Funding support Germany, 1items
OrganizationGrant numberCountry
Germany
CitationJournal: Nat Commun / Year: 2016
Title: Structure of the exportin Xpo4 in complex with RanGTP and the hypusine-containing translation factor eIF5A.
Authors: Aksu, M. / Trakhanov, S. / Gorlich, D.
History
DepositionSep 7, 2015Deposition site: RCSB / Processing site: PDBE
Revision 1.0Jun 22, 2016Provider: repository / Type: Initial release
Revision 1.1Jun 29, 2016Group: Database references
Revision 1.2Oct 16, 2024Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description / Structure summary
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / diffrn_radiation_wavelength / pdbx_entry_details / pdbx_modification_feature / struct_conn / struct_conn_type / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.conn_type_id / _struct_conn.id / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_conn_type.id / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
B: Exportin-4
C: GTP-binding nuclear protein Ran
F: Eukaryotic translation initiation factor 5A-1
A: Exportin-4
D: GTP-binding nuclear protein Ran
E: Eukaryotic translation initiation factor 5A-1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)324,44410
Polymers323,3496
Non-polymers1,0954
Water00
1
B: Exportin-4
C: GTP-binding nuclear protein Ran
F: Eukaryotic translation initiation factor 5A-1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)162,2225
Polymers161,6743
Non-polymers5472
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area7930 Å2
ΔGint-29 kcal/mol
Surface area58630 Å2
MethodPISA
2
A: Exportin-4
D: GTP-binding nuclear protein Ran
E: Eukaryotic translation initiation factor 5A-1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)162,2225
Polymers161,6743
Non-polymers5472
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area7760 Å2
ΔGint-28 kcal/mol
Surface area57920 Å2
MethodPISA
Unit cell
Length a, b, c (Å)98.616, 98.616, 726.864
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number152
Space group name H-MP3121
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain C and (resseq 7:75 or (resid 76 and (name...
21(chain D and (resseq 7:33 or (resid 34 and (name...
12(chain A and (resseq 9 or (resid 10 and (name...
22(chain B and (resseq 9 or (resid 10 and (name...
13(chain E and (resseq 16:67 or (resid 68 and (name...
23(chain F and (resseq 16:17 or (resid 18 and (name...

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111PROPROLEULEU(chain C and (resseq 7:75 or (resid 76 and (name...CB7 - 753 - 71
121ARGARGARGARG(chain C and (resseq 7:75 or (resid 76 and (name...CB7672
131PROPROMETMET(chain C and (resseq 7:75 or (resid 76 and (name...CB7 - 1793 - 175
141PROPROMETMET(chain C and (resseq 7:75 or (resid 76 and (name...CB7 - 1793 - 175
151PROPROMETMET(chain C and (resseq 7:75 or (resid 76 and (name...CB7 - 1793 - 175
161PROPROMETMET(chain C and (resseq 7:75 or (resid 76 and (name...CB7 - 1793 - 175
211PROPROGLYGLY(chain D and (resseq 7:33 or (resid 34 and (name...DE7 - 333 - 29
221GLUGLUGLUGLU(chain D and (resseq 7:33 or (resid 34 and (name...DE3430
231PROPROALAALA(chain D and (resseq 7:33 or (resid 34 and (name...DE7 - 1783 - 174
241PROPROALAALA(chain D and (resseq 7:33 or (resid 34 and (name...DE7 - 1783 - 174
251PROPROALAALA(chain D and (resseq 7:33 or (resid 34 and (name...DE7 - 1783 - 174
261PROPROALAALA(chain D and (resseq 7:33 or (resid 34 and (name...DE7 - 1783 - 174
112PROPROPROPRO(chain A and (resseq 9 or (resid 10 and (name...AD99
122GLUGLUGLUGLU(chain A and (resseq 9 or (resid 10 and (name...AD1010
132PROPROGLYGLY(chain A and (resseq 9 or (resid 10 and (name...AD9 - 11459 - 1107
142PROPROGLYGLY(chain A and (resseq 9 or (resid 10 and (name...AD9 - 11459 - 1107
152PROPROGLYGLY(chain A and (resseq 9 or (resid 10 and (name...AD9 - 11459 - 1107
212PROPROPROPRO(chain B and (resseq 9 or (resid 10 and (name...BA99
222GLUGLUGLUGLU(chain B and (resseq 9 or (resid 10 and (name...BA1010
232ALAALALEULEU(chain B and (resseq 9 or (resid 10 and (name...BA4 - 11484 - 1110
242ALAALALEULEU(chain B and (resseq 9 or (resid 10 and (name...BA4 - 11484 - 1110
252ALAALALEULEU(chain B and (resseq 9 or (resid 10 and (name...BA4 - 11484 - 1110
113ALAALALYSLYS(chain E and (resseq 16:67 or (resid 68 and (name...EF16 - 673 - 54
123LYSLYSLYSLYS(chain E and (resseq 16:67 or (resid 68 and (name...EF6855
133ALAALAMETMET(chain E and (resseq 16:67 or (resid 68 and (name...EF16 - 1523 - 139
143ALAALAMETMET(chain E and (resseq 16:67 or (resid 68 and (name...EF16 - 1523 - 139
153ALAALAMETMET(chain E and (resseq 16:67 or (resid 68 and (name...EF16 - 1523 - 139
163ALAALAMETMET(chain E and (resseq 16:67 or (resid 68 and (name...EF16 - 1523 - 139
173ALAALAMETMET(chain E and (resseq 16:67 or (resid 68 and (name...EF16 - 1523 - 139
213ALAALATHRTHR(chain F and (resseq 16:17 or (resid 18 and (name...FC16 - 173 - 4
223PHEPHEPHEPHE(chain F and (resseq 16:17 or (resid 18 and (name...FC185
233SERSERMETMET(chain F and (resseq 16:17 or (resid 18 and (name...FC15 - 1522 - 139
243SERSERMETMET(chain F and (resseq 16:17 or (resid 18 and (name...FC15 - 1522 - 139
253SERSERMETMET(chain F and (resseq 16:17 or (resid 18 and (name...FC15 - 1522 - 139
263SERSERMETMET(chain F and (resseq 16:17 or (resid 18 and (name...FC15 - 1522 - 139

NCS ensembles :
ID
1
2
3

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Components

#1: Protein Exportin-4 / Exp4


Mass: 125894.023 Da / Num. of mol.: 2 / Mutation: H1085Y
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Xpo4, Kiaa1721 / Production host: Escherichia coli K-12 (bacteria) / Variant (production host): Top10 F' / References: UniProt: Q9ESJ0
#2: Protein GTP-binding nuclear protein Ran / Androgen receptor-associated protein 24 / GTPase Ran / Ras-like protein TC4 / Ras-related nuclear protein


Mass: 20192.484 Da / Num. of mol.: 2 / Mutation: Q69L
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: RAN, ARA24, OK/SW-cl.81 / Production host: Escherichia coli K-12 (bacteria) / Variant (production host): Top10 F' / References: UniProt: P62826
#3: Protein Eukaryotic translation initiation factor 5A-1 / eIF-5A1 / Eukaryotic initiation factor 5A isoform 1 / eIF-5A / Rev-binding factor / eIF-4D


Mass: 15587.991 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: EIF5A / Production host: Escherichia coli BL21 (bacteria) / Variant (production host): NEB express / References: UniProt: P63241
#4: Chemical ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE


Mass: 523.180 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Comment: GTP, energy-carrying molecule*YM
#5: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mg
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 2

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Sample preparation

Crystal
IDDensity Matthews (Å3/Da)Density % sol (%)
13.1661.08
23.1661.1
Crystal grow
Temperature (K)Crystal-IDMethodpHDetails
2931vapor diffusion, hanging drop6.26Peg 400, MES
2932vapor diffusion, hanging drop6.26Peg 400, MES, DTT

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Data collection

Diffraction
IDMean temperature (K)Crystal-ID
11001
21002
Diffraction source
SourceSiteBeamlineIDWavelength (Å)
SYNCHROTRONSLS X10SA11
SYNCHROTRONSLS X10SA20.9786
Detector
TypeIDDetectorDate
DECTRIS PILATUS 6M-F1PIXELJun 13, 2014
DECTRIS PILATUS 6M-F2PIXELJul 10, 2014
Radiation
IDProtocolMonochromatic (M) / Laue (L)Scattering typeWavelength-ID
1SINGLE WAVELENGTHMx-ray1
2SINGLE WAVELENGTHMx-ray2
Radiation wavelength
IDWavelength (Å)Relative weight
111
20.97861
ReflectionResolution: 3→49.407 Å / Num. obs: 157964 / % possible obs: 99.9 % / Observed criterion σ(I): -3 / Redundancy: 9.9 % / Biso Wilson estimate: 106.26 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.087 / Rrim(I) all: 0.097 / Χ2: 1.008 / Net I/σ(I): 14.55 / Num. measured all: 828210
Reflection shell

Diffraction-ID: 1 / Rejects: _

Resolution (Å)Highest resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. measured obsNum. possibleNum. unique obsCC1/2Rrim(I) all% possible all
3-3.0810.21.6120.995847411673115580.4551.899
3.08-3.161.1291.476189211428114260.6591.251100
3.16-3.250.8671.895916311019110180.7730.962100
3.25-3.350.6162.625696210795107940.8340.685100
3.35-3.460.4473.555276110386103770.9020.49999.9
3.46-3.590.3414.735264610113101120.940.379100
3.59-3.720.266.2252827977197710.9650.288100
3.72-3.870.1818.6750739939993990.9830.2100
3.87-4.050.1410.9847266897489740.9880.155100
4.05-4.240.10613.7442961860786000.9920.11999.9
4.24-4.470.08317.2841815824882440.9950.092100
4.47-4.740.06721.3241014773477330.9970.075100
4.74-5.070.0623.738047717871750.9970.067100
5.07-5.480.06223.0235508687868760.9970.069100
5.48-60.05824.9131723612561250.9970.064100
6-6.710.0529.131212566456640.9980.056100
6.71-7.750.03439.9526220493849340.9990.03899.9
7.75-9.490.02157.39209114188418510.02499.9
9.49-13.420.01676.6172853240324010.018100
13.420.01479.0387841790175910.01598.3

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Processing

Software
NameVersionClassification
PHENIX1.10.1_2155refinement
XSCALEdata scaling
PDB_EXTRACT3.15data extraction
XDSdata reduction
PHENIXphasing
RefinementMethod to determine structure: SAD / Resolution: 3.2→49.407 Å / SU ML: 0.4 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 27.55 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2695 3494 5 %
Rwork0.2211 66335 -
obs0.2235 69829 99.91 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 211.92 Å2 / Biso mean: 118.5607 Å2 / Biso min: 55 Å2
Refinement stepCycle: final / Resolution: 3.2→49.407 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms20622 0 66 0 20688
Biso mean--76.87 --
Num. residues----2655
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00221055
X-RAY DIFFRACTIONf_angle_d0.50428592
X-RAY DIFFRACTIONf_chiral_restr0.0373383
X-RAY DIFFRACTIONf_plane_restr0.0033624
X-RAY DIFFRACTIONf_dihedral_angle_d9.54112654
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11C1575X-RAY DIFFRACTION9.818TORSIONAL
12D1575X-RAY DIFFRACTION9.818TORSIONAL
21A8595X-RAY DIFFRACTION9.818TORSIONAL
22B8595X-RAY DIFFRACTION9.818TORSIONAL
31E1217X-RAY DIFFRACTION9.818TORSIONAL
32F1217X-RAY DIFFRACTION9.818TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 25

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
3.2001-3.2440.33191360.30625742710100
3.244-3.29030.41681420.294826882830100
3.2903-3.33940.3371330.276525182651100
3.3394-3.39160.28581390.264826422781100
3.3916-3.44720.33571350.266425622697100
3.4472-3.50660.33531380.268726322770100
3.5066-3.57030.28511370.266426012738100
3.5703-3.6390.33091390.266426362775100
3.639-3.71320.32991360.256925872723100
3.7132-3.7940.33241400.237326632803100
3.794-3.88220.25641350.232125592694100
3.8822-3.97920.27111380.216326372775100
3.9792-4.08680.2611400.222226462786100
4.0868-4.2070.30961350.223125782713100
4.207-4.34270.2581410.218226782819100
4.3427-4.49780.26171420.193726892831100
4.4978-4.67770.23271360.188125952731100
4.6777-4.89040.25831410.19226652806100
4.8904-5.1480.26421390.20226552794100
5.148-5.47020.26381420.217226882830100
5.4702-5.89190.28271400.239326662806100
5.8919-6.48370.29351430.24827072850100
6.4837-7.41910.26721440.239427502894100
7.4191-9.3370.19671460.178327642910100
9.337-49.41290.25381570.21022955311299
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.06021.0165-0.37791.632-0.5622.9374-0.10430.9269-0.1777-0.3683-0.03090.03550.0969-0.32820.00220.44660.067-0.06510.998-0.08720.653414.086750.4646797.5276
23.7031-0.67360.07162.0930.43350.22360.020.65260.78370.403-0.0219-0.1416-0.90140.0667-0.02250.9354-0.24580.03511.16270.36470.887247.520967.5291796.4257
33.2034-0.5377-0.12481.7268-0.64321.73210.11170.53240.292-0.1847-0.1552-0.0493-0.57860.5559-0.2460.6628-0.35450.02931.10920.21670.761466.283462.3473805.5216
43.4025-1.0049-0.49142.6387-1.72431.551-0.18210.3198-0.00610.1265-0.2254-0.44360.22920.4854-0.00750.5052-0.21430.03361.1640.00670.889573.064447.7387813.0182
54.70271.4444-0.74722.9514-0.54583.25080.103-0.2872-0.30090.4771-0.138-0.36890.15820.2659-0.00150.5319-0.0532-0.10680.77660.04210.767361.399729.7011830.9725
62.49581.97632.1471.53041.67731.83630.5197-0.4349-0.23820.6357-0.4569-0.46790.3967-0.46950.00010.6978-0.2082-0.09520.64740.17870.885637.878419.3439826.7505
71.89570.03650.5341.27390.97182.6333-0.20781.0786-0.1689-0.82570.06670.9796-0.1830.0783-0.56611.0263-0.2964-0.1750.9998-0.13981.390717.495216.0198813.3165
83.98870.34760.40573.0755-0.20892.57370.20130.1316-0.2012-0.0028-0.1452-0.1642-0.1420.171300.4932-0.16550.02780.7414-0.01320.790327.588153.8965819.2743
94.33650.6473-0.85153.09540.17913.71820.0732-0.46880.29680.0016-0.3738-0.05510.1576-0.136400.99220.1364-0.03421.0637-0.02850.850547.385613.0992754.845
101.98180.5006-1.27551.9356-0.28440.7838-0.5122-0.1859-0.6283-0.6609-0.03760.09351.09691.26620.00011.56690.22430.10981.58130.16331.146977.681811.1712752.4902
111.3650.7646-0.59982.04370.38561.89580.02630.12120.1485-0.70180.01950.26960.1252-0.182-0.00011.61470.04140.22011.70560.14091.376183.526926.8083727.4372
121.48-1.74780.50292.3934-0.64592.77010.3525-0.40750.07640.2981-0.31550.6024-0.02720.25560.00011.1554-0.2455-0.04341.0630.08121.054445.194432.0034858.21
131.04870.2612-0.30961.7746-1.02982.3283-0.0341-0.06270.33420.1517-0.1173-0.3496-0.83140.54130.00020.8536-0.16340.01030.91620.0560.948351.471853.4246837.4572
141.4074-0.66630.82241.5094-0.4264.42590.0684-0.2724-0.0289-0.0131-0.29790.4249-0.0686-1.0815-0.00460.6176-0.0640.04671.4607-0.32520.897624.911718.9392766.5987
152.25021.3587-0.03660.95680.14390.1678-0.1546-0.4999-0.4177-0.171-0.17210.27850.57410.0958-0.00041.64270.2670.18711.5750.25741.032452.19466.1904788.4944
162.83930.2451-1.05970.84890.66492.8651-0.1214-1.211-0.33090.1229-0.08150.01850.55541.175-0.09021.23480.28740.08122.09040.36131.040972.813511.1771788.9303
170.63070.9039-0.08691.2636-0.28690.6963-0.0174-0.3110.40150.0212-0.0801-0.3821-0.70332.05-0.00031.23990.0503-0.12322.3633-0.05350.993383.508823.8152783.1382
182.20311.27960.82252.3795-1.15041.96980.31140.04890.3212-0.1852-0.2747-0.0589-0.25470.7648-00.8788-0.17350.13991.7196-0.12520.999182.568836.4163756.6644
191.1279-0.6676-0.41380.8289-0.40531.09440.21760.45010.1612-0.1173-0.5997-0.38960.2405-0.56620.00021.23710.19720.07710.9463-0.0191.201661.300144.9222747.9846
202.12170.96120.92151.34660.19230.4326-0.1257-0.952-0.0929-0.1641-0.00720.5076-0.7876-0.82720.00171.35390.6131-0.07591.736-0.12351.222936.308749.2687749.4132
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'B' and (resid 4 through 364 )B4 - 364
2X-RAY DIFFRACTION2chain 'B' and (resid 365 through 460 )B365 - 460
3X-RAY DIFFRACTION3chain 'B' and (resid 461 through 646 )B461 - 646
4X-RAY DIFFRACTION4chain 'B' and (resid 647 through 744 )B647 - 744
5X-RAY DIFFRACTION5chain 'B' and (resid 745 through 924 )B745 - 924
6X-RAY DIFFRACTION6chain 'B' and (resid 949 through 1015 )B949 - 1015
7X-RAY DIFFRACTION7chain 'B' and (resid 1016 through 1148 )B0
8X-RAY DIFFRACTION8chain 'C'C7 - 179
9X-RAY DIFFRACTION9chain 'D'D7 - 178
10X-RAY DIFFRACTION10chain 'E' and (resid 16 through 83 )E16 - 83
11X-RAY DIFFRACTION11chain 'E' and (resid 84 through 152 )E84 - 152
12X-RAY DIFFRACTION12chain 'F' and (resid 84 through 152 )F84 - 152
13X-RAY DIFFRACTION13chain 'F' and (resid 15 through 83 )F15 - 83
14X-RAY DIFFRACTION14chain 'A' and (resid 9 through 364 )A9 - 364
15X-RAY DIFFRACTION15chain 'A' and (resid 365 through 451 )A365 - 451
16X-RAY DIFFRACTION16chain 'A' and (resid 460 through 646 )A460 - 646
17X-RAY DIFFRACTION17chain 'A' and (resid 647 through 744 )A647 - 744
18X-RAY DIFFRACTION18chain 'A' and (resid 745 through 922 )A745 - 922
19X-RAY DIFFRACTION19chain 'A' and (resid 952 through 1015 )A952 - 1015
20X-RAY DIFFRACTION20chain 'A' and (resid 1016 through 1145 )A0

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