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Open data
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Basic information
Entry | Database: PDB / ID: 3fay | ||||||
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Title | Crystal structure of the GAP-related domain of IQGAP1 | ||||||
![]() | Ras GTPase-activating-like protein IQGAP1 | ||||||
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Function / homology | ![]() negative regulation of dephosphorylation / podocyte development / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kurella, V.B. / Richard, J.M. / Parke, C.L. / Bellamy, H. / Worthylake, D.K. | ||||||
![]() | ![]() Title: Crystal structure of the GTPase-activating protein-related domain from IQGAP1. Authors: Kurella, V.B. / Richard, J.M. / Parke, C.L. / Lecour, L.F. / Bellamy, H.D. / Worthylake, D.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 87.2 KB | Display | ![]() |
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PDB format | ![]() | 70.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 302.1 KB | Display | ![]() |
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Full document | ![]() | 311.8 KB | Display | |
Data in XML | ![]() | 18.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 44535.023 Da / Num. of mol.: 1 / Fragment: GAP-related domain (GRD) Source method: isolated from a genetically manipulated source Details: ligation independent cloning vector / Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-TRS / ![]() |
#3: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.39 Å3/Da / Density % sol: 48.5 % |
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Crystal grow![]() | Temperature: 277 K / Method: vapor diffusion / pH: 8.5 Details: 20% PEG 2000 methyl ether, 500mM MgCl2, 100mM Tris HCL, pH 8.5, VAPOR DIFFUSION, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||
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Diffraction source | Source: ![]() ![]() | ||||||||||||
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Aug 20, 2006 | ||||||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2.2→25 Å / Num. all: 20692 / Num. obs: 20692 / % possible obs: 86.1 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 5.6 % / Rsym value: 0.062 / Net I/σ(I): 8.9 | ||||||||||||
Reflection shell | Resolution: 2.2→2.28 Å / Mean I/σ(I) obs: 2.1 / Rsym value: 0.242 / % possible all: 38.2 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Displacement parameters | Biso mean: 47.3 Å2 | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→25 Å
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