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Open data
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Basic information
Entry | Database: PDB / ID: 3enc | ||||||
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Title | Crystal structure of Pyrococcus furiosus PCC1 dimer | ||||||
![]() | protein PCC1 | ||||||
![]() | UNKNOWN FUNCTION / dimerization domain / KEOPS / telomere | ||||||
Function / homology | CTAG/Pcc1 family / Transcription factor Pcc1 / Alpha-D-phosphohexomutase, C-terminal domain / TATA-Binding Protein / 2-Layer Sandwich / Alpha Beta / KEOPS complex subunit Pcc1![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Neculai, D. | ||||||
![]() | ![]() Title: Atomic structure of the KEOPS complex: an ancient protein kinase-containing molecular machine. Authors: Mao, D.Y. / Neculai, D. / Downey, M. / Orlicky, S. / Haffani, Y.Z. / Ceccarelli, D.F. / Ho, J.S. / Szilard, R.K. / Zhang, W. / Ho, C.S. / Wan, L. / Fares, C. / Rumpel, S. / Kurinov, I. / ...Authors: Mao, D.Y. / Neculai, D. / Downey, M. / Orlicky, S. / Haffani, Y.Z. / Ceccarelli, D.F. / Ho, J.S. / Szilard, R.K. / Zhang, W. / Ho, C.S. / Wan, L. / Fares, C. / Rumpel, S. / Kurinov, I. / Arrowsmith, C.H. / Durocher, D. / Sicheri, F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 73.7 KB | Display | ![]() |
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PDB format | ![]() | 60.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 436.7 KB | Display | ![]() |
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Full document | ![]() | 448 KB | Display | |
Data in XML | ![]() | 9.4 KB | Display | |
Data in CIF | ![]() | 11.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 10041.199 Da / Num. of mol.: 2 / Fragment: pcc1 / Mutation: I12M Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.68 Å3/Da / Density % sol: 54.09 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.1 Details: 45% PEG 300 0.1 M Na/K phosphate, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Nov 10, 2007 / Details: MIRRORS |
Radiation | Monochromator: HORIZONTAL BENT SI(111), ASYMMETRICALLY CUT WITH WATER COOLED CU BLOCK Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97935 Å / Relative weight: 1 |
Reflection twin | Type: pseudo-merohedral / Operator: h,-h-k,-l / Fraction: 0.503 |
Reflection | Resolution: 2.6304→68.251 Å / Num. all: 6413 / Num. obs: 6401 / % possible obs: 99.8 % / Observed criterion σ(I): 2.48 / Redundancy: 1.93 % / Rmerge(I) obs: 0.0331 / Rsym value: 0.0305 / Net I/σ(I): 20.16 |
Reflection shell | Resolution: 2.6304→2.72 Å / Redundancy: 1.93 % / Rmerge(I) obs: 0.2677 / Mean I/σ(I) obs: 2.86 / Num. unique all: 612 / Rsym value: 0.398 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 138.453 Å2 / ksol: 0.356 e/Å3 | ||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.6304→68.251 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 14.1503 Å / Origin y: 24.3766 Å / Origin z: 30.0971 Å
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Refinement TLS group | Selection details: all |