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Yorodumi- PDB-3ejm: Crystal structure of the mimivirus NDK +Kpn mutant complexed with GDP -
+Open data
-Basic information
Entry | Database: PDB / ID: 3ejm | ||||||
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Title | Crystal structure of the mimivirus NDK +Kpn mutant complexed with GDP | ||||||
Components | Nucleoside diphosphate kinase | ||||||
Keywords | TRANSFERASE / NDK Mimivirus phosphotransferase nucleotide binding / ATP-binding / Kinase / Magnesium / Metal-binding / Nucleotide metabolism / Nucleotide-binding / Phosphoprotein | ||||||
Function / homology | Function and homology information nucleoside-diphosphate kinase / UTP biosynthetic process / CTP biosynthetic process / GTP biosynthetic process / nucleoside diphosphate kinase activity / ATP binding / metal ion binding Similarity search - Function | ||||||
Biological species | Acanthamoeba polyphaga mimivirus | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.95 Å | ||||||
Authors | Jeudy, S. / Lartigue, A. / Claverie, J.M. / Abergel, C. | ||||||
Citation | Journal: J.Virol. / Year: 2009 Title: Dissecting the unique nucleotide specificity of mimivirus nucleoside diphosphate kinase. Authors: Jeudy, S. / Lartigue, A. / Claverie, J.M. / Abergel, C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3ejm.cif.gz | 74.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3ejm.ent.gz | 54.4 KB | Display | PDB format |
PDBx/mmJSON format | 3ejm.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 3ejm_validation.pdf.gz | 1.1 MB | Display | wwPDB validaton report |
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Full document | 3ejm_full_validation.pdf.gz | 1.1 MB | Display | |
Data in XML | 3ejm_validation.xml.gz | 15.1 KB | Display | |
Data in CIF | 3ejm_validation.cif.gz | 21.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ej/3ejm ftp://data.pdbj.org/pub/pdb/validation_reports/ej/3ejm | HTTPS FTP |
-Related structure data
Related structure data | 2b8pSC 2b8qC 3b6bC 3ddiC 3dkdC 3ee3C 3eicC 3elhC 3em1C 3emtC 3enaC 3etmC 3evmC 3evoC 3evwC 3fbbC 3fbcC 3fbeC 3fbfC 3fc9C 3fcvC 3fcwC 3g2xC 3gp9C 3gpaC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 16628.791 Da / Num. of mol.: 2 / Mutation: residues 91 to 94 [NILT] replaced with [NTNPLASA] Source method: isolated from a genetically manipulated source Source: (gene. exp.) Acanthamoeba polyphaga mimivirus / Gene: NDK, MIMI_R418 / Plasmid: pDIGS02 / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta(DE3)pLysS / References: UniProt: Q5UQL3, nucleoside-diphosphate kinase #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | Sequence details | RESIDUES 91 TO 94 [NILT] REPLACED WITH [NTNPLASA] | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.24 Å3/Da / Density % sol: 45.12 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.6 Details: 1.1M sodium citrate, 0.1M Tris, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: BM30A / Wavelength: 0.98 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Aug 7, 2007 / Details: Two mirrors |
Radiation | Monochromator: either 111 or 311 Silicon single cristals / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 1.95→30 Å / Num. obs: 23045 / % possible obs: 100 % / Redundancy: 6.5 % / Biso Wilson estimate: 24.505 Å2 / Rsym value: 0.082 / Net I/σ(I): 6.5 |
Reflection shell | Resolution: 1.95→2.06 Å / Redundancy: 4.9 % / Mean I/σ(I) obs: 1.9 / Rsym value: 0.051 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2b8p Resolution: 1.95→22.963 Å / Occupancy max: 1 / Occupancy min: 1 / SU ML: 0.22 / σ(F): 1.35 / Phase error: 20.49 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 43.201 Å2 / ksol: 0.397 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 24.946 Å2
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Refinement step | Cycle: LAST / Resolution: 1.95→22.963 Å
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Refine LS restraints |
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LS refinement shell |
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