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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 3cms | ||||||
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タイトル | ENGINEERING ENZYME SUB-SITE SPECIFICITY: PREPARATION, KINETIC CHARACTERIZATION AND X-RAY ANALYSIS AT 2.0-ANGSTROMS RESOLUTION OF VAL111PHE SITE-MUTATED CALF CHYMOSIN | ||||||
![]() | CHYMOSIN B | ||||||
![]() | HYDROLASE / ACID PROTEINASE | ||||||
機能・相同性 | ![]() | ||||||
生物種 | ![]() ![]() | ||||||
手法 | ![]() | ||||||
![]() | Newman, M. / Frazao, C. / Shearer, A. / Tickle, I.J. / Blundell, T.L. | ||||||
![]() | ![]() タイトル: Engineering enzyme subsite specificity: preparation, kinetic characterization, and X-ray analysis at 2.0-A resolution of Val111Phe site-mutated calf chymosin. 著者: Strop, P. / Sedlacek, J. / Stys, J. / Kaderabkova, Z. / Blaha, I. / Pavlickova, L. / Pohl, J. / Fabry, M. / Kostka, V. / Newman, M. #1: ![]() タイトル: X-Ray Analyses of Aspartic Proteinases Iv: Structure and Refinement at 2.2 Angstroms Resolution of Bovine Chymosin 著者: Newman, M. / Safro, M. / Frazao, C. / Kahn, G. / Zdanov, A. / Tickle, I.J. / Blundell, T.L. / Andreeva, N. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 73.1 KB | 表示 | ![]() |
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PDB形式 | ![]() | 56.8 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 369.8 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 388.7 KB | 表示 | |
XML形式データ | ![]() | 10.3 KB | 表示 | |
CIF形式データ | ![]() | 15.5 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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Atom site foot note | 1: RESIDUE PRO 23 IS A CIS-PROLINE. 2: THESE RESIDUES ARE IN POORLY DEFINED REGIONS IN THE ELECTRON DENSITY MAP. 3: RESIDUES 72 TO 79 HAVE BEEN REFINED IN TWO ALTERNATE CONFORMATIONS. THESE RESIDUES ARE IN POORLY DEFINED REGIONS IN THE ELECTRON DENSITY MAP. CONFORMATION B HAS THE HIGHER RELATIVE OCCUPANCY (O.60) ...3: RESIDUES 72 TO 79 HAVE BEEN REFINED IN TWO ALTERNATE CONFORMATIONS. THESE RESIDUES ARE IN POORLY DEFINED REGIONS IN THE ELECTRON DENSITY MAP. CONFORMATION B HAS THE HIGHER RELATIVE OCCUPANCY (O.60) AND IS ASSOCIATED WITH SUPERIOR ELECTRON DENSITY. THUS IT APPEARS THAT CONFORMATION B IS SIGNIFICANTLY MORE HIGHLY POPULATED THAN CONFORMATION A. CONFORMATION A WITH OCCUPANCY OF 0.40 MUST BE REGARDED AS A TENTATIVE INTERPRETATION. |
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要素
#1: タンパク質 | 分子量: 35720.738 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() |
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#2: 水 | ChemComp-HOH / |
構成要素の詳細 | THE SPECIFIC GENE MUTATION V111F IS SITUATED BETWEEN THE PRIMARY SPECIFICITY BINDING POCKET S1 AND ...THE SPECIFIC GENE MUTATION V111F IS SITUATED BETWEEN THE PRIMARY SPECIFICIT |
-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.33 Å3/Da / 溶媒含有率: 47.16 % | ||||||||||||||||||||||||
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結晶化 | *PLUS pH: 6.5 / 手法: microdialysis | ||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
放射 | 散乱光タイプ: x-ray |
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放射波長 | 相対比: 1 |
反射 | *PLUS 最高解像度: 2 Å / Num. obs: 22098 / % possible obs: 96.4 % / Observed criterion σ(I): 0.072 / Num. measured all: 149939 / Rmerge(I) obs: 3 |
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解析
ソフトウェア | 名称: RESTRAIN / 分類: 精密化 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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精密化 | 解像度: 2→10 Å 詳細: THE QUANTITY PRESENTED IN THE TEMPERATURE FACTOR FIELD IS U. RESIDUES 72 TO 79 HAVE BEEN REFINED IN TWO ALTERNATE CONFORMATIONS. TURN 5 WITH THE A CONFORMATION IS CLASSIFED TYPE II' 2:2 ...詳細: THE QUANTITY PRESENTED IN THE TEMPERATURE FACTOR FIELD IS U. RESIDUES 72 TO 79 HAVE BEEN REFINED IN TWO ALTERNATE CONFORMATIONS. TURN 5 WITH THE A CONFORMATION IS CLASSIFED TYPE II' 2:2 DISTORTED. TURN 5 WITH THE B CONFORMATION IS UNCLASSIFIED 2:2. INVARIANT RESIDUE TYR 14 HAS BEEN BUILT INTO A CONFORMATION THAT DIFFERS FROM THE WILD-TYPE CHYMOSIN STRUCTURE. HOWEVER, THIS RESIDUE BELONGS TO THE POORLY DEFINED SURFACE REGION BETWEEN STRANDS AN AND BN, AND THUS THE ASSIGNMENT OF ITS POSITION MUST BE REGARDED AS TENTATIVE.
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精密化ステップ | サイクル: LAST / 解像度: 2→10 Å
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拘束条件 |
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精密化 | *PLUS Rfactor obs: 0.195 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS Biso mean: 30.3 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 | *PLUS タイプ: p_plane_restr / Dev ideal: 0.019 |