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Yorodumi- PDB-3cfp: Structure of the replicating complex of a POL Alpha family DNA Po... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3cfp | ||||||
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Title | Structure of the replicating complex of a POL Alpha family DNA Polymerase, ternary complex 1 | ||||||
Components |
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Keywords | Transferase/DNA / DNA POLYMERASE / CATALYTIC COMPLEX / FIDELITY / TWO METAL ION MECHANISM / DNA replication / DNA-binding / DNA-directed DNA polymerase / Exonuclease / Hydrolase / Nuclease / Nucleotidyltransferase / Transferase / Transferase-DNA COMPLEX | ||||||
Function / homology | Function and homology information bidirectional double-stranded viral DNA replication / Hydrolases; Acting on ester bonds; Exodeoxyribonucleases producing 5'-phosphomonoesters / 3'-5' exonuclease activity / DNA replication / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / nucleotide binding / DNA binding / metal ion binding Similarity search - Function | ||||||
Biological species | Bacteriophage RB69 (virus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.5 Å | ||||||
Authors | Wang, J. / Klimenko, D. / Wang, M. / Steitz, T.A. / Konigsberg, W.H. | ||||||
Citation | Journal: To be Published Title: Insights into base selectivity from the structures of an RB69 DNA Polymerase triple mutant Authors: Klimenko, D. / Wang, M. / Steitz, T.A. / Konigsberg, W.H. / Wang, J. #1: Journal: Cell(Cambridge,Mass.) / Year: 1997 Title: Crystal structure of a POL Alpha family replicative DNA Polymerase from bacteriophage RB69 Authors: Wang, J. / Sattar, A.K. / Wang, C.C. / Karam, J.D. / Konigsberg, W.H. / Steitz, T.A. / Franklin, M.C. #2: Journal: Cell(Cambridge,Mass.) / Year: 2001 Title: Structure of the replicating complex of a POL Alpha DNA polymerase Authors: Franklin, M.C. / Wang, J. / Steitz, T.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3cfp.cif.gz | 224.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3cfp.ent.gz | 172.9 KB | Display | PDB format |
PDBx/mmJSON format | 3cfp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cf/3cfp ftp://data.pdbj.org/pub/pdb/validation_reports/cf/3cfp | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-DNA chain , 2 types, 2 molecules TP
#1: DNA chain | Mass: 5550.605 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: SYNTHEsized by W.M. KECK FACILITY AT YALE UNIVERSITY) |
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#2: DNA chain | Mass: 4240.768 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: SYNTHEsized by W.M. KECK FACILITY AT YALE UNIVERSITY) |
-Protein , 1 types, 1 molecules A
#3: Protein | Mass: 105407.852 Da / Num. of mol.: 1 / Mutation: L561A, S565G, Y567A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacteriophage RB69 (virus) / Gene: 43 / Production host: Escherichia coli (E. coli) / References: UniProt: Q38087, DNA-directed DNA polymerase |
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-Non-polymers , 4 types, 305 molecules
#4: Chemical | ChemComp-CA / #5: Chemical | ChemComp-CL / | #6: Chemical | ChemComp-TTP / | #7: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.61 Å3/Da / Density % sol: 52.79 % | ||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 7% PEG350 monomethyl ether, 0.1M NA CACODYLATE, 0.14M CACL2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||||||
Components of the solutions |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 24-ID-C |
Detector | Detector: CCD / Date: Feb 2, 2007 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 2.5→50 Å / Num. obs: 42496 / % possible obs: 99.8 % / Observed criterion σ(I): 1 / Redundancy: 6.6 % / Rmerge(I) obs: 0.088 |
Reflection shell | Resolution: 2.5→2.8 Å / Redundancy: 5 % / Rmerge(I) obs: 0.1045 / Num. unique all: 9961 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.5→50 Å / Cor.coef. Fo:Fc: 0.957 / Cor.coef. Fo:Fc free: 0.921 / SU B: 25.497 / SU ML: 0.276 / TLS residual ADP flag: LIKELY RESIDUAL / Cross valid method: THROUGHOUT / ESU R: 0.56 / ESU R Free: 0.31 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 54.811 Å2
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Refinement step | Cycle: LAST / Resolution: 2.5→50 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.5→2.563 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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