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Open data
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Basic information
Entry | Database: PDB / ID: 3bh6 | ||||||
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Title | Crystal structure of the RP2-Arl3 complex bound to GppNHp | ||||||
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![]() | SIGNALING PROTEIN / PROTEIN-PROTEIN COMPLEX / GTPASE ACTIVATING PROTEIN AND GTPASE / RETINITIS PIGMENTOSA / GTP-BINDING / LIPOPROTEIN / MYRISTATE / NUCLEOTIDE-BINDING / DISEASE MUTATION / MEMBRANE / PALMITATE / PHOSPHOPROTEIN / SENSORY TRANSDUCTION / VISION / METAL BINDING PROTEIN | ||||||
Function / homology | ![]() Trafficking of myristoylated proteins to the cilium / Trafficking of myristoylated proteins to the cilium / photoreceptor cell development / periciliary membrane compartment / protein localization to ciliary membrane / intraciliary transport / post-Golgi vesicle-mediated transport / ciliary transition zone / photoreceptor connecting cilium / Golgi to plasma membrane transport ...Trafficking of myristoylated proteins to the cilium / Trafficking of myristoylated proteins to the cilium / photoreceptor cell development / periciliary membrane compartment / protein localization to ciliary membrane / intraciliary transport / post-Golgi vesicle-mediated transport / ciliary transition zone / photoreceptor connecting cilium / Golgi to plasma membrane transport / smoothened signaling pathway / small GTPase-mediated signal transduction / cytoplasmic microtubule / axoneme / mitotic cytokinesis / cilium assembly / visual perception / centriole / GTPase activator activity / ciliary basal body / kidney development / spindle microtubule / cilium / GDP binding / microtubule cytoskeleton / unfolded protein binding / protein transport / protein folding / midbody / cytoplasmic vesicle / microtubule binding / nuclear body / Golgi membrane / GTPase activity / centrosome / GTP binding / Golgi apparatus / magnesium ion binding / extracellular exosome / nucleoplasm / nucleus / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Veltel, S. / Gasper, R. / Wittinghofer, A. | ||||||
![]() | ![]() Title: The retinitis pigmentosa 2 gene product is a GTPase-activating protein for Arf-like 3 Authors: Veltel, S. / Gasper, R. / Eisenacher, E. / Wittinghofer, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 110 KB | Display | ![]() |
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PDB format | ![]() | 81.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 754.9 KB | Display | ![]() |
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Full document | ![]() | 764.1 KB | Display | |
Data in XML | ![]() | 19.9 KB | Display | |
Data in CIF | ![]() | 27.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3bh7C ![]() 1ksgS ![]() 2bx6S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 18369.828 Da / Num. of mol.: 1 / Fragment: residues 14-177 / Mutation: Q71L Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Protein | Mass: 39827.098 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
#3: Chemical | ChemComp-MG / |
#4: Chemical | ChemComp-GNP / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.45 Å3/Da / Density % sol: 49.8 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 20% PEG 3350, 0.2 M potassium chloride, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Nov 26, 2005 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9792 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→19.81 Å / Num. all: 18118 / Num. obs: 18118 / % possible obs: 99.6 % / Observed criterion σ(I): 3.8 / Redundancy: 5.9 % / Biso Wilson estimate: 36.068 Å2 / Rsym value: 0.082 / Net I/σ(I): 16.12 |
Reflection shell | Resolution: 2.6→2.7 Å / Redundancy: 4.6 % / Mean I/σ(I) obs: 3.75 / Num. unique all: 1919 / Rsym value: 0.435 / % possible all: 99.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1KSG, 2BX6 Resolution: 2.6→19.81 Å / Cor.coef. Fo:Fc: 0.924 / Cor.coef. Fo:Fc free: 0.879 / SU B: 11.431 / SU ML: 0.246 / Cross valid method: THROUGHOUT / ESU R: 1.133 / ESU R Free: 0.353 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 36.051 Å2
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Refinement step | Cycle: LAST / Resolution: 2.6→19.81 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.6→2.666 Å / Total num. of bins used: 20
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