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Open data
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Basic information
Entry | Database: PDB / ID: 2zlt | ||||||
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Title | Horse methemoglobin high salt, pH 7.0 | ||||||
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![]() | Oxygen storage / Oxygen transport / protein hydration / allosteric transitions / variability in quaternary structure / solvent content and crystal structure / water-mediated transformation / Heme / Iron / Metal-binding / Polymorphism / Transport | ||||||
Function / homology | ![]() cellular oxidant detoxification / hemoglobin alpha binding / haptoglobin-hemoglobin complex / organic acid binding / hemoglobin complex / hydrogen peroxide catabolic process / oxygen carrier activity / oxygen binding / iron ion binding / heme binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Kaushal, P.S. / Sankaranarayanan, R. / Vijayan, M. | ||||||
![]() | ![]() Title: Water-mediated variability in the structure of relaxed-state haemoglobin Authors: Kaushal, P.S. / Sankaranarayanan, R. / Vijayan, M. #1: ![]() Title: A new relaxed state in horse methemoglobin characterized by crystallographic studies Authors: Sankaranarayanan, R. / Biswal, B.K. / Vijayan, M. #2: ![]() Title: Structures of human oxy- and deoxyhaemoglobin at different levels of humidity: variability in the T state Authors: Biswal, B.K. / Vijayan, M. #3: ![]() Title: Structure of human methaemoglobin: The variation of a theme Authors: Biswal, B.K. / Vijayan, M. #4: ![]() Title: The structure of hemoglobin III: Direct determination of the molecular transform Authors: Perutz, M.F. #5: ![]() Title: Discontinuous lattice changes in hemoglobin crystal Authors: Huxley, H.E. / Kendrew, J.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 75.9 KB | Display | ![]() |
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PDB format | ![]() | 55.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 2zluC ![]() 2zlvC ![]() 2zlwC ![]() 2zlxC ![]() 2mhbS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 15138.280 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() | ||
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#2: Protein | Mass: 16032.274 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.83 Å3/Da / Density % sol: 56.61 % |
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Crystal grow | Temperature: 298 K / Method: liquid diffusion / pH: 7 Details: 2.6M ammonium sulfate, 0.01M ammonium phosphate, pH 7.0, LIQUID DIFFUSION, temperature 298.0K |
-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Apr 12, 2003 / Details: OSMIC MIRROR |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→18.32 Å / Num. obs: 26933 / % possible obs: 97.6 % / Redundancy: 3.5 % / Biso Wilson estimate: 39.6 Å2 / Rmerge(I) obs: 0.136 / Net I/σ(I): 8.7 |
Reflection shell | Resolution: 1.9→1.97 Å / Rmerge(I) obs: 0.528 / Mean I/σ(I) obs: 3.3 / % possible all: 99.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2MHB Resolution: 1.9→18.32 Å / Cor.coef. Fo:Fc: 0.95 / Cor.coef. Fo:Fc free: 0.925 / Cross valid method: THROUGHOUT / ESU R: 0.147 / ESU R Free: 0.137 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 32.588 Å2
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Refinement step | Cycle: LAST / Resolution: 1.9→18.32 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.9→1.949 Å / Total num. of bins used: 20
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