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- PDB-2xtp: Crystal structure of nucleotide-free human GIMAP2, amino acid res... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2xtp | ||||||
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Title | Crystal structure of nucleotide-free human GIMAP2, amino acid residues 1-260 | ||||||
![]() | GTPASE IMAP FAMILY MEMBER 2 | ||||||
![]() | IMMUNE SYSTEM / G PROTEIN | ||||||
Function / homology | ![]() lipid droplet / GTP binding / endoplasmic reticulum / nucleoplasm / identical protein binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Schwefel, D. / Froehlich, C. / Daumke, O. | ||||||
![]() | ![]() Title: Structural Basis of Oligomerization in Septin-Like Gtpase of Immunity-Associated Protein 2 (Gimap2) Authors: Schwefel, D. / Froehlich, C. / Eichhorst, J. / Wiesner, B. / Behlke, J. / Aravind, L. / Daumke, O. #1: Journal: Acta Crystallogr.,Sect.F / Year: 2010 Title: Purification, Crystallization and Preliminary X- Ray Analysis of Human Gimap2. Authors: Schwefel, D. / Frohlich, C. / Daumke, O. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 97.6 KB | Display | ![]() |
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PDB format | ![]() | 80.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 406.9 KB | Display | ![]() |
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Full document | ![]() | 409.3 KB | Display | |
Data in XML | ![]() | 11.7 KB | Display | |
Data in CIF | ![]() | 16.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 29354.859 Da / Num. of mol.: 1 / Fragment: RESIDUES 1-260 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.15 Å3/Da / Density % sol: 42.62 % / Description: NONE |
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Crystal grow | pH: 7.5 / Details: 11-12% 2-PROPANOL, 15% GLYCEROL, pH 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||
Detector | Type: MARRESEARCH / Detector: CCD / Date: Jan 23, 2008 | |||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||
Radiation wavelength |
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Reflection | Resolution: 1.75→50 Å / Num. obs: 183804 / % possible obs: 99.1 % / Observed criterion σ(I): 1 / Redundancy: 3.8 % / Biso Wilson estimate: 26.9 Å2 / Rmerge(I) obs: 0.07 / Net I/σ(I): 14.6 | |||||||||
Reflection shell | Resolution: 1.75→1.8 Å / Redundancy: 3.3 % / Rmerge(I) obs: 0.5 / Mean I/σ(I) obs: 3.2 / % possible all: 95.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: NONE Resolution: 1.5→19.14 Å / Cor.coef. Fo:Fc: 0.959 / Cor.coef. Fo:Fc free: 0.951 / SU B: 2.353 / SU ML: 0.04 / Cross valid method: THROUGHOUT / ESU R: 0.069 / ESU R Free: 0.07 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 12.251 Å2
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Refinement step | Cycle: LAST / Resolution: 1.5→19.14 Å
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Refine LS restraints |
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