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Open data
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Basic information
Entry | Database: PDB / ID: 2x1o | ||||||
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Title | Gelsolin Nanobody | ||||||
![]() | GELSOLIN NANOBODY | ||||||
![]() | CONTRACTILE PROTEIN | ||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Van Den Abbeele, A. / Declercq, S. / De Ganck, A. / De Corte, V. / Van Loo, B. / Srinivasan, V. / Steyaert, J. / Van De Kerckhove, J. / Gettemans, J. | ||||||
![]() | ![]() Title: A Llama-Derived Gelsolin Single-Domain Antibody Blocks Gelsolin-G-Actin Interaction. Authors: Van Den Abbeele, A. / De Clercq, S. / De Ganck, A. / De Corte, V. / Van Loo, B. / Soror, S.H. / Srinivasan, V. / Steyaert, J. / Vandekerckhove, J. / Gettemans, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 60.7 KB | Display | ![]() |
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PDB format | ![]() | 49 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 13440.044 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.67 Å3/Da / Density % sol: 53.59 % / Description: NONE |
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Crystal grow | Details: 30% PEG 1000, 100 MM PHOSPHATE/CITRATE PH 4.4, 100 MM LISO4 |
-Data collection
Diffraction | Mean temperature: 287 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.984 Å / Relative weight: 1 |
Reflection | Resolution: 1.34→20 Å / Num. obs: 59490 / % possible obs: 87.4 % / Observed criterion σ(I): 1 / Redundancy: 2.5 % / Rmerge(I) obs: 0.07 |
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Processing
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Refinement | Method to determine structure: OTHER Starting model: NONE Resolution: 1.34→20 Å / Cor.coef. Fo:Fc: 0.955 / Cor.coef. Fo:Fc free: 0.942 / SU B: 0.863 / SU ML: 0.037 / Cross valid method: THROUGHOUT / ESU R: 0.063 / ESU R Free: 0.066 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 19.512 Å2
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Refinement step | Cycle: LAST / Resolution: 1.34→20 Å
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Refine LS restraints |
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