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Open data
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Basic information
Entry | Database: PDB / ID: 2x1q | ||||||
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Title | Gelsolin Nanobody | ||||||
![]() | GELSOLIN NANOBODY | ||||||
![]() | CONTRACTILE PROTEIN | ||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Van Den Abbeele, A. / Declercq, S. / De Ganck, A. / De Corte, V. / Van Loo, B. / Srinivasan, V. / Steyaert, J. / Van De Kerckhove, J. / Gettemans, J. | ||||||
![]() | ![]() Title: A Llama-Derived Gelsolin Single-Domain Antibody Blocks Gelsolin-G-Actin Interaction. Authors: Van Den Abbeele, A. / De Clercq, S. / De Ganck, A. / De Corte, V. / Van Loo, B. / Soror, S.H. / Srinivasan, V. / Steyaert, J. / Vandekerckhove, J. / Gettemans, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 68.7 KB | Display | ![]() |
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PDB format | ![]() | 50.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 443.5 KB | Display | ![]() |
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Full document | ![]() | 452 KB | Display | |
Data in XML | ![]() | 16.5 KB | Display | |
Data in CIF | ![]() | 24.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2x1oC ![]() 2x1pC ![]() 1hcvS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 13906.346 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.76 Å3/Da / Density % sol: 29.48 % / Description: NONE |
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Crystal grow | Details: 30% PEG 1000, 100 MM PHOSPHATE/ CITRATE PH 4.4, 100 MM LISO4. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9834 Å / Relative weight: 1 |
Reflection | Resolution: 1.06→20 Å / Num. obs: 92321 / % possible obs: 91.3 % / Observed criterion σ(I): 1 / Redundancy: 3.9 % / Rmerge(I) obs: 0.07 / Net I/σ(I): 15.2 |
Reflection shell | Resolution: 1.06→1.1 Å / Redundancy: 3.2 % / Rmerge(I) obs: 0.34 / Mean I/σ(I) obs: 3.78 / % possible all: 77.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1HCV Resolution: 1.06→50 Å / Cor.coef. Fo:Fc: 0.936 / Cor.coef. Fo:Fc free: 0.927 / SU B: 0.945 / SU ML: 0.025 / Cross valid method: THROUGHOUT / ESU R: 0.041 / ESU R Free: 0.041 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 12.76 Å2
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Refinement step | Cycle: LAST / Resolution: 1.06→50 Å
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Refine LS restraints |
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