+Open data
-Basic information
Entry | Database: PDB / ID: 2wxz | ||||||
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Title | Crystal structure of rat angiotensinogen in C2 space group | ||||||
Components | (ANGIOTENSINOGEN) x 2 | ||||||
Keywords | HORMONE / ANGIOTENSINOGEN / RENIN / ANGIOTENSIN / HYPERTENSION / GLYCOPROTEIN / VASOCONSTRICTOR / VASOACTIVE | ||||||
Function / homology | Function and homology information renal response to blood flow involved in circulatory renin-angiotensin regulation of systemic arterial blood pressure / negative regulation of tissue remodeling / uterine smooth muscle contraction / positive regulation of L-lysine import across plasma membrane / establishment of blood-nerve barrier / positive regulation of L-arginine import across plasma membrane / aldosterone secretion / smooth muscle cell proliferation / regulation of systemic arterial blood pressure by circulatory renin-angiotensin / brain renin-angiotensin system ...renal response to blood flow involved in circulatory renin-angiotensin regulation of systemic arterial blood pressure / negative regulation of tissue remodeling / uterine smooth muscle contraction / positive regulation of L-lysine import across plasma membrane / establishment of blood-nerve barrier / positive regulation of L-arginine import across plasma membrane / aldosterone secretion / smooth muscle cell proliferation / regulation of systemic arterial blood pressure by circulatory renin-angiotensin / brain renin-angiotensin system / ovarian follicle rupture / regulation of transmission of nerve impulse / response to muscle activity involved in regulation of muscle adaptation / type 2 angiotensin receptor binding / maintenance of blood vessel diameter homeostasis by renin-angiotensin / Metabolism of Angiotensinogen to Angiotensins / negative regulation of neurotrophin TRK receptor signaling pathway / positive regulation of extracellular matrix constituent secretion / Peptide ligand-binding receptors / vasopressin secretion / cell growth involved in cardiac muscle cell development / regulation of extracellular matrix assembly / operant conditioning / vascular associated smooth muscle cell proliferation / regulation of renal output by angiotensin / regulation of norepinephrine secretion / artery smooth muscle contraction / renin-angiotensin regulation of aldosterone production / renal system process / angiotensin-mediated vasoconstriction involved in regulation of systemic arterial blood pressure / drinking behavior / peristalsis / positive regulation of branching involved in ureteric bud morphogenesis / positive regulation of macrophage derived foam cell differentiation / positive regulation of organ growth / positive regulation of extracellular matrix assembly / smooth muscle cell differentiation / positive regulation of fatty acid biosynthetic process / positive regulation of blood pressure / intracellular sodium ion homeostasis / vasoconstriction / positive regulation of multicellular organism growth / hormone metabolic process / type 1 angiotensin receptor binding / positive regulation of extrinsic apoptotic signaling pathway / cellular response to angiotensin / response to angiotensin / positive regulation of vascular associated smooth muscle cell migration / G alpha (q) signalling events / G alpha (i) signalling events / positive regulation of epidermal growth factor receptor signaling pathway / organ growth / branching involved in ureteric bud morphogenesis / positive regulation of cardiac muscle cell apoptotic process / blood vessel development / positive regulation of cardiac muscle hypertrophy / negative regulation of vascular associated smooth muscle cell proliferation / associative learning / regulation of calcium ion transport / angiotensin-mediated drinking behavior / positive regulation of gap junction assembly / regulation of cardiac conduction / positive regulation of insulin receptor signaling pathway / positive regulation of epithelial to mesenchymal transition / stress-activated MAPK cascade / response to mechanical stimulus / response to salt stress / positive regulation of vascular associated smooth muscle cell proliferation / positive regulation of protein metabolic process / ERK1 and ERK2 cascade / regulation of heart rate / positive regulation of endothelial cell migration / response to cold / extracellular matrix organization / negative regulation of angiogenesis / cell-matrix adhesion / positive regulation of superoxide anion generation / kidney development / positive regulation of cytokine production / angiotensin-activated signaling pathway / female pregnancy / astrocyte activation / negative regulation of smooth muscle cell proliferation / serine-type endopeptidase inhibitor activity / regulation of long-term neuronal synaptic plasticity / hormone activity / negative regulation of cell growth / positive regulation of neuron projection development / regulation of blood pressure / positive regulation of miRNA transcription / cellular response to mechanical stimulus / vasodilation / protein import into nucleus / positive regulation of nitric oxide biosynthetic process / MAPK cascade / positive regulation of fibroblast proliferation / response to estradiol / positive regulation of peptidyl-serine phosphorylation / positive regulation of cytosolic calcium ion concentration / regulation of inflammatory response Similarity search - Function | ||||||
Biological species | RATTUS NORVEGICUS (Norway rat) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.8 Å | ||||||
Authors | Zhou, A. / Wei, Z. / Carrell, R.W. / Read, R.J. | ||||||
Citation | Journal: Nature / Year: 2010 Title: A Redox Switch in Angiotensinogen Modulates Angiotensin Release. Authors: Zhou, A. / Carrell, R.W. / Murphy, M.P. / Wei, Z. / Yan, Y. / Stanley, P.L. / Stein, P.E. / Pipkin, F.B. / Read, R.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2wxz.cif.gz | 326.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2wxz.ent.gz | 278.6 KB | Display | PDB format |
PDBx/mmJSON format | 2wxz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2wxz_validation.pdf.gz | 439 KB | Display | wwPDB validaton report |
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Full document | 2wxz_full_validation.pdf.gz | 458.7 KB | Display | |
Data in XML | 2wxz_validation.xml.gz | 30.3 KB | Display | |
Data in CIF | 2wxz_validation.cif.gz | 40.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wx/2wxz ftp://data.pdbj.org/pub/pdb/validation_reports/wx/2wxz | HTTPS FTP |
-Related structure data
Related structure data | 2wxwC 2wxxC 2wxyC 2wy0C 2wy1C 2x0bC C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
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-Components
#1: Protein | Mass: 49556.211 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) RATTUS NORVEGICUS (Norway rat) / Plasmid: PSUMO3-MANGT / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P01015 |
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#2: Protein | Mass: 49588.277 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) RATTUS NORVEGICUS (Norway rat) / Plasmid: PSUMO3-MANGT / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P01015 |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 49 % / Description: NONE |
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Crystal grow | pH: 7.8 / Details: 20-25% PEG3350, 0.2M MGSO4, 0.1M TRIS -HCL, PH7.8 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I02 / Wavelength: 1.045 |
Detector | Type: ADSC CCD / Detector: CCD / Date: Jul 1, 2008 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.045 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→41.6 Å / Num. obs: 23736 / % possible obs: 98.5 % / Observed criterion σ(I): 2 / Redundancy: 4.7 % / Rmerge(I) obs: 0.22 / Net I/σ(I): 8 |
Reflection shell | Resolution: 2.8→2.95 Å / Redundancy: 4.1 % / Rmerge(I) obs: 1.04 / Mean I/σ(I) obs: 1.2 / % possible all: 96.8 |
-Processing
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Refinement | Method to determine structure: SAD Starting model: NONE Resolution: 2.8→50 Å / Cor.coef. Fo:Fc: 0.883 / Cor.coef. Fo:Fc free: 0.868 / SU B: 60.573 / SU ML: 0.512 / Cross valid method: THROUGHOUT / ESU R Free: 0.478 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 23.2 Å2
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Refinement step | Cycle: LAST / Resolution: 2.8→50 Å
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Refine LS restraints |
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