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Open data
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Basic information
Entry | Database: PDB / ID: 2wxz | ||||||
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Title | Crystal structure of rat angiotensinogen in C2 space group | ||||||
![]() | (ANGIOTENSINOGEN) x 2 | ||||||
![]() | HORMONE / ANGIOTENSINOGEN / RENIN / ANGIOTENSIN / HYPERTENSION / GLYCOPROTEIN / VASOCONSTRICTOR / VASOACTIVE | ||||||
Function / homology | ![]() renal response to blood flow involved in circulatory renin-angiotensin regulation of systemic arterial blood pressure / negative regulation of tissue remodeling / uterine smooth muscle contraction / positive regulation of L-lysine import across plasma membrane / establishment of blood-nerve barrier / positive regulation of L-arginine import across plasma membrane / aldosterone secretion / smooth muscle cell proliferation / regulation of systemic arterial blood pressure by circulatory renin-angiotensin / brain renin-angiotensin system ...renal response to blood flow involved in circulatory renin-angiotensin regulation of systemic arterial blood pressure / negative regulation of tissue remodeling / uterine smooth muscle contraction / positive regulation of L-lysine import across plasma membrane / establishment of blood-nerve barrier / positive regulation of L-arginine import across plasma membrane / aldosterone secretion / smooth muscle cell proliferation / regulation of systemic arterial blood pressure by circulatory renin-angiotensin / brain renin-angiotensin system / ovarian follicle rupture / regulation of transmission of nerve impulse / response to muscle activity involved in regulation of muscle adaptation / : / type 2 angiotensin receptor binding / Metabolism of Angiotensinogen to Angiotensins / maintenance of blood vessel diameter homeostasis by renin-angiotensin / positive regulation of extracellular matrix constituent secretion / negative regulation of neurotrophin TRK receptor signaling pathway / Peptide ligand-binding receptors / vasopressin secretion / cell growth involved in cardiac muscle cell development / operant conditioning / regulation of extracellular matrix assembly / vascular associated smooth muscle cell proliferation / regulation of renal output by angiotensin / regulation of norepinephrine secretion / artery smooth muscle contraction / renal system process / renin-angiotensin regulation of aldosterone production / angiotensin-mediated vasoconstriction involved in regulation of systemic arterial blood pressure / drinking behavior / peristalsis / positive regulation of organ growth / positive regulation of branching involved in ureteric bud morphogenesis / positive regulation of extracellular matrix assembly / smooth muscle cell differentiation / positive regulation of macrophage derived foam cell differentiation / positive regulation of fatty acid biosynthetic process / positive regulation of blood pressure / positive regulation of multicellular organism growth / vasoconstriction / intracellular sodium ion homeostasis / hormone metabolic process / type 1 angiotensin receptor binding / positive regulation of extrinsic apoptotic signaling pathway / G alpha (q) signalling events / cellular response to angiotensin / response to angiotensin / positive regulation of vascular associated smooth muscle cell migration / G alpha (i) signalling events / positive regulation of epidermal growth factor receptor signaling pathway / organ growth / branching involved in ureteric bud morphogenesis / positive regulation of cardiac muscle hypertrophy / blood vessel development / positive regulation of cardiac muscle cell apoptotic process / angiotensin-mediated drinking behavior / negative regulation of vascular associated smooth muscle cell proliferation / associative learning / regulation of calcium ion transport / positive regulation of gap junction assembly / regulation of cardiac conduction / positive regulation of epithelial to mesenchymal transition / response to mechanical stimulus / positive regulation of insulin receptor signaling pathway / stress-activated MAPK cascade / positive regulation of vascular associated smooth muscle cell proliferation / response to salt stress / positive regulation of protein metabolic process / ERK1 and ERK2 cascade / regulation of heart rate / response to cold / positive regulation of endothelial cell migration / negative regulation of angiogenesis / extracellular matrix organization / cell-matrix adhesion / positive regulation of superoxide anion generation / kidney development / positive regulation of cytokine production / astrocyte activation / angiotensin-activated signaling pathway / female pregnancy / negative regulation of smooth muscle cell proliferation / regulation of long-term neuronal synaptic plasticity / serine-type endopeptidase inhibitor activity / hormone activity / negative regulation of cell growth / regulation of blood pressure / positive regulation of neuron projection development / positive regulation of miRNA transcription / cellular response to mechanical stimulus / vasodilation / protein import into nucleus / MAPK cascade / positive regulation of nitric oxide biosynthetic process / positive regulation of fibroblast proliferation / positive regulation of peptidyl-serine phosphorylation / response to estradiol / positive regulation of cytosolic calcium ion concentration Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zhou, A. / Wei, Z. / Carrell, R.W. / Read, R.J. | ||||||
![]() | ![]() Title: A Redox Switch in Angiotensinogen Modulates Angiotensin Release. Authors: Zhou, A. / Carrell, R.W. / Murphy, M.P. / Wei, Z. / Yan, Y. / Stanley, P.L. / Stein, P.E. / Pipkin, F.B. / Read, R.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 326.8 KB | Display | ![]() |
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PDB format | ![]() | 278.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 439 KB | Display | ![]() |
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Full document | ![]() | 458.7 KB | Display | |
Data in XML | ![]() | 30.3 KB | Display | |
Data in CIF | ![]() | 40.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2wxwC ![]() 2wxxC ![]() 2wxyC ![]() 2wy0C ![]() 2wy1C ![]() 2x0bC C: citing same article ( |
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Similar structure data |
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
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Components
#1: Protein | Mass: 49556.211 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Protein | Mass: 49588.277 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 49 % / Description: NONE |
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Crystal grow | pH: 7.8 / Details: 20-25% PEG3350, 0.2M MGSO4, 0.1M TRIS -HCL, PH7.8 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Jul 1, 2008 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.045 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→41.6 Å / Num. obs: 23736 / % possible obs: 98.5 % / Observed criterion σ(I): 2 / Redundancy: 4.7 % / Rmerge(I) obs: 0.22 / Net I/σ(I): 8 |
Reflection shell | Resolution: 2.8→2.95 Å / Redundancy: 4.1 % / Rmerge(I) obs: 1.04 / Mean I/σ(I) obs: 1.2 / % possible all: 96.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: NONE Resolution: 2.8→50 Å / Cor.coef. Fo:Fc: 0.883 / Cor.coef. Fo:Fc free: 0.868 / SU B: 60.573 / SU ML: 0.512 / Cross valid method: THROUGHOUT / ESU R Free: 0.478 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 23.2 Å2
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Refinement step | Cycle: LAST / Resolution: 2.8→50 Å
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Refine LS restraints |
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