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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 2wtt | ||||||
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タイトル | Structure of the human p73 tetramerization domain (crystal form II) | ||||||
![]() | TUMOR PROTEIN P73 | ||||||
![]() | TRANSCRIPTION / ALTERNATIVE SPLICING / OLIGOMERIZATION DOMAIN / CELL-CYCLE CONTROL / TRANSCRIPTION FACTOR / COOPERATIVITY / PHOSPHOPROTEIN / UBL CONJUGATION / ACTIVATOR / TUMOR SUPPRESSION / DEVELOPMENT / APOPTOSIS / CELL CYCLE / DNA BINDING / TRANSCRIPTION REGULATION | ||||||
機能・相同性 | ![]() positive regulation of lung ciliated cell differentiation / negative regulation of cardiac muscle cell proliferation / TP53 Regulates Transcription of Death Receptors and Ligands / Activation of PUMA and translocation to mitochondria / Regulation of TP53 Activity through Association with Co-factors / positive regulation of oligodendrocyte differentiation / TP53 regulates transcription of several additional cell death genes whose specific roles in p53-dependent apoptosis remain uncertain / TP53 Regulates Transcription of Caspase Activators and Caspases / TP53 Regulates Transcription of Genes Involved in Cytochrome C Release / negative regulation of neuron differentiation ...positive regulation of lung ciliated cell differentiation / negative regulation of cardiac muscle cell proliferation / TP53 Regulates Transcription of Death Receptors and Ligands / Activation of PUMA and translocation to mitochondria / Regulation of TP53 Activity through Association with Co-factors / positive regulation of oligodendrocyte differentiation / TP53 regulates transcription of several additional cell death genes whose specific roles in p53-dependent apoptosis remain uncertain / TP53 Regulates Transcription of Caspase Activators and Caspases / TP53 Regulates Transcription of Genes Involved in Cytochrome C Release / negative regulation of neuron differentiation / intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / mismatch repair / MDM2/MDM4 family protein binding / : / regulation of mitotic cell cycle / transcription corepressor binding / kidney development / protein tetramerization / intrinsic apoptotic signaling pathway in response to DNA damage / p53 binding / cell junction / RUNX1 regulates transcription of genes involved in differentiation of HSCs / regulation of gene expression / DNA-binding transcription activator activity, RNA polymerase II-specific / DNA-binding transcription factor binding / RNA polymerase II-specific DNA-binding transcription factor binding / positive regulation of MAPK cascade / regulation of cell cycle / DNA-binding transcription factor activity, RNA polymerase II-specific / transcription cis-regulatory region binding / response to xenobiotic stimulus / positive regulation of apoptotic process / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of cell population proliferation / intracellular membrane-bounded organelle / DNA damage response / chromatin / regulation of transcription by RNA polymerase II / protein kinase binding / positive regulation of DNA-templated transcription / Golgi apparatus / positive regulation of transcription by RNA polymerase II / nucleoplasm / identical protein binding / nucleus / metal ion binding / cytosol 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() ![]() | ||||||
![]() | Joerger, A.C. | ||||||
![]() | ![]() タイトル: Structural Evolution of P53, P63, and P73: Implication for Heterotetramer Formation. 著者: Joerger, A.C. / Rajagopalan, S. / Natan, E. / Veprintsev, D.B. / Robinson, C.V. / Fersht, A.R. | ||||||
履歴 |
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Remark 650 | HELIX DETERMINATION METHOD: AUTHOR PROVIDED. |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 150.7 KB | 表示 | ![]() |
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PDB形式 | ![]() | 122.8 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 530 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 541.5 KB | 表示 | |
XML形式データ | ![]() | 25.1 KB | 表示 | |
CIF形式データ | ![]() | 36 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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要素
#1: タンパク質 | 分子量: 6225.732 Da / 分子数: 16 / 断片: TETRAMERIZATION DOMAIN, RESIDUES 351-399 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() #2: 水 | ChemComp-HOH / | 配列の詳細 | TWO ADDITIONAL | |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2 Å3/Da / 溶媒含有率: 39.8 % / 解説: NONE |
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結晶化 | 温度: 290 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 6.2 詳細: SITTING DROP VAPOR DIFFUSION AT 17 DEGREE C. PROTEIN SOLUTION: 15 MG/ML IN 20 MM TRIS (PH 8.5), 50 MM NACL. CRYSTALLIZATION BUFFER: 0.1 M SODIUM CITRATE (PH 6.2), 40% PEG 600. |
-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | タイプ: ADSC CCD / 検出器: CCD |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.9791 Å / 相対比: 1 |
反射 | 解像度: 2.3→59.7 Å / Num. obs: 36567 / % possible obs: 100 % / Observed criterion σ(I): 3 / 冗長度: 11.6 % / Biso Wilson estimate: 34.87 Å2 / Rmerge(I) obs: 0.09 / Net I/σ(I): 15.7 |
反射 シェル | 解像度: 2.3→2.42 Å / 冗長度: 11.9 % / Rmerge(I) obs: 0.354 / Mean I/σ(I) obs: 5.9 / % possible all: 100 |
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解析
ソフトウェア | 名称: PHENIX / バージョン: (PHENIX.REFINE) / 分類: 精密化 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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精密化 | 構造決定の手法: ![]() 開始モデル: PDB ENTRY 2WQI 解像度: 2.3→24.786 Å / SU ML: 0.39 / σ(F): 1.19 / 位相誤差: 28.37 / 立体化学のターゲット値: ML
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溶媒の処理 | 減衰半径: 0.9 Å / VDWプローブ半径: 1.11 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL / Bsol: 62.313 Å2 / ksol: 0.366 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 39.6 Å2
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精密化ステップ | サイクル: LAST / 解像度: 2.3→24.786 Å
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拘束条件 |
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LS精密化 シェル |
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