- PDB-2wng: complete extracellular structure of human signal regulatory prote... -
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ID or keywords:
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Basic information
Entry
Database: PDB / ID: 2wng
Title
complete extracellular structure of human signal regulatory protein (SIRP) alpha
Components
TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE SUBSTRATE 1
Keywords
CELL ADHESION / SIGNAL REGULATORY PROTEIN ALPHA / IMMUNOGLOBULIN SUPERFAMILY / PHOSPHOPROTEIN / DISULFIDE BOND / PAIRED RECEPTOR / ALTERNATIVE SPLICING / IMMUNOGLOBULIN DOMAIN / SIRP / CD47 / SIRPA / MEMBRANE / SH3-BINDING / POLYMORPHISM / GLYCOPROTEIN / TRANSMEMBRANE
Function / homology
Function and homology information
negative regulation of I-kappaB phosphorylation / cellular response to interleukin-12 / monocyte extravasation / negative regulation of macrophage inflammatory protein 1 alpha production / negative regulation of chemokine (C-C motif) ligand 5 production / protein binding involved in heterotypic cell-cell adhesion / regulation of interleukin-1 beta production / regulation of type II interferon production / GTPase regulator activity / cell-cell adhesion mediator activity ...negative regulation of I-kappaB phosphorylation / cellular response to interleukin-12 / monocyte extravasation / negative regulation of macrophage inflammatory protein 1 alpha production / negative regulation of chemokine (C-C motif) ligand 5 production / protein binding involved in heterotypic cell-cell adhesion / regulation of interleukin-1 beta production / regulation of type II interferon production / GTPase regulator activity / cell-cell adhesion mediator activity / protein antigen binding / negative regulation of nitric oxide biosynthetic process / negative regulation of interferon-beta production / negative regulation of JNK cascade / regulation of tumor necrosis factor production / regulation of nitric oxide biosynthetic process / negative regulation of phagocytosis / regulation of interleukin-6 production / Signal regulatory protein family interactions / negative regulation of interleukin-6 production / tertiary granule membrane / ficolin-1-rich granule membrane / negative regulation of tumor necrosis factor production / negative regulation of cytokine production involved in inflammatory response / cellular response to interleukin-1 / positive regulation of phagocytosis / protein tyrosine kinase binding / negative regulation of protein phosphorylation / Cell surface interactions at the vascular wall / negative regulation of ERK1 and ERK2 cascade / negative regulation of inflammatory response / cellular response to type II interferon / SH3 domain binding / cellular response to hydrogen peroxide / cell migration / positive regulation of T cell activation / regulation of gene expression / cellular response to lipopolysaccharide / protein phosphatase binding / cell adhesion / Neutrophil degranulation / cell surface / extracellular exosome / membrane / plasma membrane Similarity search - Function
Mass: 18.015 Da / Num. of mol.: 49 / Source method: isolated from a natural source / Formula: H2O
Sequence details
FINAL 8 RESIDUES (TRHHHHHH) ARE DERIVED FROM THE EXPRESSION VECTOR.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.85 Å3/Da / Density % sol: 56.9 % / Description: NONE
Crystal grow
Method: vapor diffusion, sitting drop / pH: 6.5 Details: SITTING DROPS CONTAINING 100 NL SIRP (18.6 MG/ML) PLUS 100 NL PRECIPITANT (1.0 M TRI-SODIUM CITRATE, 0.1 M SODIUM CACODYLATE, PH 6.5) WERE EQUILIBRATED AT 20.5C AGAINST 95 UL RESERVOIRS OF ...Details: SITTING DROPS CONTAINING 100 NL SIRP (18.6 MG/ML) PLUS 100 NL PRECIPITANT (1.0 M TRI-SODIUM CITRATE, 0.1 M SODIUM CACODYLATE, PH 6.5) WERE EQUILIBRATED AT 20.5C AGAINST 95 UL RESERVOIRS OF PRECIPITANT. CRYSTALS WERE CRYO-PROTECTED BY A QUICK SWEEP THROUGH PERFLUOROPOLYETHER PFO-X125/03 (LANCASTER SYNTHESIS).
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