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Yorodumi- PDB-2wgz: Crystal structure of alpha-1,3 galactosyltransferase (alpha3GT) i... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2wgz | |||||||||
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Title | Crystal structure of alpha-1,3 galactosyltransferase (alpha3GT) in a complex with p-nitrophenyl-beta-galactoside (pNP-beta-Gal) | |||||||||
Components | N-ACETYLLACTOSAMINIDE ALPHA-1,3-GALACTOSYL TRANSFERASE | |||||||||
Keywords | TRANSFERASE / GALACTOSYLTRANSFERASE / GLYCOPROTEIN / METAL-BINDING / SIGNAL-ANCHOR / ALPHA-1 / MEMBRANE / MANGANESE / SUBSTRATE BINDING / GLYCOSYLTRANSFERASE / TRANSMEMBRANE / GOLGI APPARATUS / ENZYME KINETICS | |||||||||
Function / homology | Function and homology information N-acetyllactosaminide 3-alpha-galactosyltransferase / N-acetyllactosaminide 3-alpha-galactosyltransferase activity / Golgi cisterna / lipid glycosylation / Golgi cisterna membrane / glycosyltransferase activity / protein glycosylation / vesicle / carbohydrate metabolic process / Golgi apparatus / metal ion binding Similarity search - Function | |||||||||
Biological species | BOS TAURUS (cattle) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.12 Å | |||||||||
Authors | Jamaluddin, H. / Tumbale, P. / Ferns, T.A. / Thiyagarajan, N. / Brew, K. / Acharya, K.R. | |||||||||
Citation | Journal: Biochem.Biophys.Res.Commun. / Year: 2009 Title: Crystal Structure of Alpha-1,3 Galactosyltransferase (Alpha3Gt) in a Complex with P-Nitrophenyl-Beta-Galactoside (Pnpbetagal). Authors: Jamaluddin, H. / Tumbale, P. / Ferns, T.A. / Thiyagarajan, N. / Brew, K. / Acharya, K.R. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2wgz.cif.gz | 147.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2wgz.ent.gz | 113.8 KB | Display | PDB format |
PDBx/mmJSON format | 2wgz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2wgz_validation.pdf.gz | 1.3 MB | Display | wwPDB validaton report |
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Full document | 2wgz_full_validation.pdf.gz | 1.3 MB | Display | |
Data in XML | 2wgz_validation.xml.gz | 28.7 KB | Display | |
Data in CIF | 2wgz_validation.cif.gz | 42.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wg/2wgz ftp://data.pdbj.org/pub/pdb/validation_reports/wg/2wgz | HTTPS FTP |
-Related structure data
Related structure data | 1k4vS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 34247.359 Da / Num. of mol.: 2 / Fragment: RESIDUES 80-368 Source method: isolated from a genetically manipulated source Source: (gene. exp.) BOS TAURUS (cattle) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) References: UniProt: P14769, EC: 2.4.1.151, N-acetyllactosaminide 3-alpha-galactosyltransferase |
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-Sugars , 2 types, 2 molecules
#2: Sugar | ChemComp-147 / |
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#6: Sugar | ChemComp-GAL / |
-Non-polymers , 5 types, 560 molecules
#3: Chemical | #4: Chemical | #5: Chemical | ChemComp-GOL / #7: Chemical | ChemComp-NPO / | #8: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.9 Å3/Da / Density % sol: 43 % / Description: NONE |
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Crystal grow | pH: 8 Details: 5% PEG 6000, 0.1M TRIS-HCL, PH 8.0 IN THE PRESENCE OF 10MM MNCL2 AND 10MM UDP |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SRS / Beamline: PX14.1 / Wavelength: 1.488 |
Detector | Type: ADSC CCD / Detector: CCD / Date: Oct 5, 2006 / Details: MIRRORS |
Radiation | Monochromator: SI (111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.488 Å / Relative weight: 1 |
Reflection | Resolution: 2.12→50 Å / Num. obs: 44578 / % possible obs: 94.4 % / Observed criterion σ(I): 0 / Redundancy: 3.1 % / Biso Wilson estimate: 22.9 Å2 / Rmerge(I) obs: 0.09 / Net I/σ(I): 12.4 |
Reflection shell | Resolution: 2.12→2.2 Å / Redundancy: 3 % / Rmerge(I) obs: 0.22 / Mean I/σ(I) obs: 5.14 / % possible all: 80.7 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1K4V Resolution: 2.12→25.29 Å / Cor.coef. Fo:Fc: 0.954 / Cor.coef. Fo:Fc free: 0.928 / SU B: 3.665 / SU ML: 0.099 / Cross valid method: THROUGHOUT / ESU R: 0.197 / ESU R Free: 0.164 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 18.96 Å2
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Refinement step | Cycle: LAST / Resolution: 2.12→25.29 Å
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