+Open data
-Basic information
Entry | Database: PDB / ID: 2w29 | ||||||
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Title | Gly102Thr mutant of Rv3291c | ||||||
Components | PROBABLE TRANSCRIPTIONAL REGULATORY PROTEIN | ||||||
Keywords | TRANSCRIPTION / DNA-BINDING / MUTANT / TRANSCRITION REGULATOR / TRANSCRIPTION REGULATION | ||||||
Function / homology | Function and homology information amino acid binding / response to amino acid / protein homooligomerization / sequence-specific DNA binding / DNA binding / cytosol Similarity search - Function | ||||||
Biological species | MYCOBACTERIUM TUBERCULOSIS (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 4.1 Å | ||||||
Authors | Shrivastava, T. / Dey, S. / Ravishankar, R. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2009 Title: Ligand-Induced Structural Transitions, Mutational Analysis, and 'Open' Quaternary Structure of the M. Tuberculosis Feast/Famine Regulatory Protein (Rv3291C). Authors: Shrivastava, T. / Dey, A. / Ramachandran, R. | ||||||
History |
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Remark 700 | SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AB" IN EACH CHAIN ON SHEET RECORDS BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AB" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "CB" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2w29.cif.gz | 119 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2w29.ent.gz | 96.1 KB | Display | PDB format |
PDBx/mmJSON format | 2w29.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2w29_validation.pdf.gz | 426.5 KB | Display | wwPDB validaton report |
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Full document | 2w29_full_validation.pdf.gz | 451.8 KB | Display | |
Data in XML | 2w29_validation.xml.gz | 17.3 KB | Display | |
Data in CIF | 2w29_validation.cif.gz | 23.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/w2/2w29 ftp://data.pdbj.org/pub/pdb/validation_reports/w2/2w29 | HTTPS FTP |
-Related structure data
Related structure data | 2w24C 2w25C 2ivmS 2w28 S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 16574.648 Da / Num. of mol.: 4 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) MYCOBACTERIUM TUBERCULOSIS (bacteria) / Strain: H37RV / Plasmid: PET21D / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21 / References: UniProt: P96896 Compound details | ENGINEERED RESIDUE IN CHAIN A, GLY 102 TO THR ENGINEERED RESIDUE IN CHAIN B, GLY 102 TO THR ...ENGINEERED | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.67 Å3/Da / Density % sol: 66.23 % / Description: NONE |
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Crystal grow | pH: 6 / Details: pH 6.0 |
-Data collection
Diffraction | Mean temperature: 287 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU300 / Wavelength: 1.5418 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Details: MIRROR |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 4.1→70 Å / Num. obs: 23928 / % possible obs: 94.4 % / Observed criterion σ(I): 2 / Redundancy: 3.2 % / Rmerge(I) obs: 0.14 / Net I/σ(I): 8.6 |
Reflection shell | Resolution: 4.1→4.32 Å / Redundancy: 2.6 % / Rmerge(I) obs: 0.41 / Mean I/σ(I) obs: 2.2 / % possible all: 83.7 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2IVM Resolution: 4.1→20 Å / Cross valid method: 2 / σ(F): 2 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Refinement step | Cycle: LAST / Resolution: 4.1→20 Å
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