TIPAN IS THE N-TERMINAL DOMAIN OF TIPAL. RESIDUE M1 WAS CLEAVED DURING EXPRESSION. THE SEQUENCE IS ...TIPAN IS THE N-TERMINAL DOMAIN OF TIPAL. RESIDUE M1 WAS CLEAVED DURING EXPRESSION. THE SEQUENCE IS DESCRIBED IN HOLMES ET AL. (1993) EMBO J, VOL. 12, P. 3183. THE CRYSTAL CONTAINED DOUBLE-STRANDED DNA WITH SEQUENCES TTGCACCTCACGTCACGTGAGGAGGC (PLUS STRAND) AND AGCCTCCTCACGTGACGTGAGGTGCA (MINUS STRAND). BOTH STRANDS WERE MODELED AS CHAIN B WITH SEQUENCE CTCCTCACGTCACGTGAGGTG.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.83 Å3/Da / Density % sol: 60 % / Description: NONE
Resolution: 2.9→30 Å / Cor.coef. Fo:Fc: 0.923 / Cor.coef. Fo:Fc free: 0.872 / SU B: 56.04 / SU ML: 0.423 / TLS residual ADP flag: LIKELY RESIDUAL / Cross valid method: THROUGHOUT / ESU R: 1.777 / ESU R Free: 0.401 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. ATOM RECORD CONTAINS RESIDUAL B FACTORS ONLY.
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.274
230
4.5 %
RANDOM
Rwork
0.239
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obs
0.24
4922
95.9 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK