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Open data
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Basic information
Entry | Database: PDB / ID: 5fd5 | ||||||
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Title | manganese uptake regulator | ||||||
![]() | Ferric uptake regulation protein | ||||||
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Function / homology | ![]() DNA-binding transcription factor activity / ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Bellini, D. / Lebedev, A. / Keegan, R. / Walsh, M.A. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structure of an apo metal-free manganese uptake regulator, mur Authors: Bellini, D. / Lebedev, A. / Keegan, R. / Todd, J. / Hemmings, A.M. / Johnston, A.W. / Walsh, M.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 156.3 KB | Display | ![]() |
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PDB format | ![]() | 125.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 16580.717 Da / Num. of mol.: 5 / Fragment: UNP residues 2-142 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: fur / Production host: ![]() ![]() ![]() #2: Chemical | ChemComp-SO4 / ![]() #3: Chemical | ![]() #4: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.93 Å3/Da / Density % sol: 68.72 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 0.1 M sodium acetate pH 4.75, 0.8 M ammonium sulfate and 2 M NaCl |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Dec 1, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.91→54 Å / Num. obs: 97820 / % possible obs: 99.7 % / Redundancy: 4.4 % / Rmerge(I) obs: 0.036 / Net I/σ(I): 29.6 |
Reflection shell | Highest resolution: 1.91 Å / Rmerge(I) obs: 0.54 / Mean I/σ(I) obs: 2.2 / % possible all: 99.9 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 38.611 Å2
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Refinement step | Cycle: LAST / Resolution: 1.91→53.92 Å
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Refine LS restraints |
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